优化Fe-CA仿酶合成工艺研究

为了减少梗丝中木质素含量,改善梗丝的品质,提高其在卷烟中的应用,对Fe-CA仿酶合成的4个影响因素(摩尔配比、反应温度、反应时间、pH)进行单因素试验和正交试验优化,并将合成的Fe-CA仿酶对梗丝进行木质素降解,对处理前后的梗丝进行电镜扫描,并将其制成单料烟进行感官评价。结果表明,最佳合成方案条件是n(Fe~(2+))∶n(柠檬酸)为1∶1,反应温度是60℃,反应时间是40 min,pH值为6.0,此时合成率为82.1%;FeCA仿酶处理后的梗丝,木质素含量由3.49%降至1.60%,去除率达54.15%;Fe-CA仿酶处理后的梗丝表面变得更加齐整光滑,毛突减少;与对照相比,试验卷烟的香气质和香气量得到了改善,木质杂气减少,刺激性降低,品质得到提升。

Optimization of the synthesis process of Fe-CA biomimetic enzyme

In order to reduce the lignin content in cut stems,to improve the quality of cut stems and to increase their proportion in cigarette blends,single factor experiments were conducted to study the effects of four factors involved in synthesis of Fe-CA biomimetic (molar ratio,reaction temperature,reaction time,and pH).The synthesis process of Fe-CA biomimetic was optimized by orthogonal test,the cut stems were analyzed by scanning electron microscope before and after Fe-CA biomimetic treatment,and the sensory quality of cigarettes made of the treated and untreated cut stems was panel tested,separately.The results showed that:under the optimum synthesis conditions,n (Fe2 +):n (citric acid) 11,reaction temperature 60 ℃,reaction time 40 min,and pH 6.0,the synthesis rate is 82.1％;after the Fe-CA biomimetic treatment of the cut stems,the content of lignin in cut stems fell from 3.49％ to 1.60％,the removal rate reached 54.15％,and the surface of the cut stems was smoother with less burrs;compared with the control,the aroma quality,aroma quantity of the test cigarette smoke were improved,the levels of offensive odor and irritation were reduced,and the quality of the cut stems was enhanced.