| 粉红聚端孢菌胞外几丁质酶纯化、特性及抗菌活性 |
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| 引用本文： | 张世宏,李多川,魏毅,刘开启. 粉红聚端孢菌胞外几丁质酶纯化、特性及抗菌活性[J]. 植物病理学报, 2002, 32(3): 262-266 |
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| 作者姓名： | 张世宏 李多川 魏毅 刘开启 |
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| 作者单位： | 1 山东农业大学植保系, 泰安 271018;2 山东棉花研究中心, 济南 250100 |
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| 基金项目： | 国家自然科学基金资助项目 (3970 0 0 97) |
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| 摘 要： | 粉红聚端孢菌(Trichothecium roseum)在SMCS中恒温摇床(200 r/min,28℃)上培养12 d,诱导产生了大量的胞外几丁质酶。培养滤液经(NH4)2SO4分级沉淀、DEAE-Sepharose FF阴离子交换柱层析和CM-Sepharose FF阳离子交换柱层析获得了SDS-PAGE纯的胞外几丁质酶,分子量约为39k Da。几丁质酶最适作用温度为40℃,最适pH范围为4.0~7.0。抗菌活性显示,纯酶对供试病原菌都有不同程度的抑菌作用,其中对棉花黄萎菌的抑制作用最强。
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| 关 键 词： | 粉红聚端孢菌 几丁质酶 纯化 特性 抗菌 |
| 文章编号： | 0412-0914(2002)03-0262-05 |
| 修稿时间： | 2000-10-08 |
PURIFICATION,PROPERTIES AND ANTIFUNGAL ACTIVITY OF AN EXTRACELLULAR CHITINASE PRODUCED BY Trichothecium roseum |
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| ZHANG Shi hong ,LI Duo chuan ,WEI Yi ,LIU Kai qi. PURIFICATION,PROPERTIES AND ANTIFUNGAL ACTIVITY OF AN EXTRACELLULAR CHITINASE PRODUCED BY Trichothecium roseum[J]. Acta Phytopathologica Sinica, 2002, 32(3): 262-266 |
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| Authors: | ZHANG Shi hong LI Duo chuan WEI Yi LIU Kai qi |
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| Affiliation: | 1 Shandong Agricultural University, Taian 271018;2 Shandong Cotton Research Center, Jinan 250100, China |
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| Abstract: | From the culture filtrate of the Trichothecium roseum, an extra cellular chitinase was purified to SDS-PAGE homogenous by ammonium sulfate fraction, DEAE-Sepharose FF chromatography and CM-Sepharose FF chromatography. The molecular weight of the chitinase was about 39 kDa. The optimum temperature was 40℃. The optimum activity was at pH 4.0-7.0. The chitinase appeared to be antifungal against some fungi and more effective against Verticillium dahliae. |
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| Keywords: | Trichothecium roseum chitinase purification properties antifungal |
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