| NPY对犊牛脂肪细胞HSL和ACC的影响 |
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| 引用本文： | 张才,杨自军,王哲,刘国文.NPY对犊牛脂肪细胞HSL和ACC的影响[J].饲料工业,2011,32(15). |
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| 作者姓名： | 张才 杨自军 王哲 刘国文 |
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| 作者单位： | 1. 河南科技大学动物科技学院,471003
2. 吉林大学畜牧兽医学院 |
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| 基金项目： | 教育部高等学校博士点基金项目资助[20070183156] |
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| 摘 要： | 试验探讨NPY在脂肪动员中的作用。体外培养犊牛脂肪细胞,通过添加不同浓度的外源性NPY(0、50、100、200、500、1 000 pg/ml)观察对脂肪细胞脂肪动员关键酶的影响。结果表明:①NPY降低脂肪细胞HSL mRNA的水平和活性,具有一定的剂量依赖性;②NPY可直接上调ACC的表达,并具一定的剂量依赖性;③随着NPY添加时间的延长,脂肪细胞HSL mRNA的水平和活性逐渐降低;④随着NPY添加时间的延长,脂肪细胞ACC的表达也逐渐升高。因此,NPY可在一定程度上促进脂肪合成,抑制脂肪动员的发生。
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| 关 键 词： | NPY 脂肪细胞 HSL ACC |
Effect of NPY on HSL and ACC of bovine adipocyte |
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| Zhang Cai,Yang Zijun,Wang Zhe,Liu Guowen.Effect of NPY on HSL and ACC of bovine adipocyte[J].Feed Industry,2011,32(15). |
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| Authors: | Zhang Cai Yang Zijun Wang Zhe Liu Guowen |
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| Institution: | Zhang Cai,Yang Zijun,Wang Zhe,Liu Guowen |
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| Abstract: | The focal point of this study is to probe the regulation mechanism of NPY on the above mentioned metabolism.Bovine preadipocyte was cultured by the method of adipocyte culture reported by previous literature.The effect of NPY(0,50,100,200,500,1 000 pg/ml)on mRNA expression of HSL,ACC in adipocyte were detected by fluorescent quantitation PCR.The result showed that: the expression levels of HSL mRNA in fat cell were decreased with increasing concentration of NPY in culture media.Enzyme activity of HSL was al... |
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| Keywords: | NPY adipocyte HSL ACC |
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