影响番茄离体培养再生的主要因素探讨

以5个番茄品种的子叶和下胚轴为外植体,对无菌苗的生理状态、激素组合、激素浓度和基因型等影响组培效果的主要因素进行探讨,建立了番茄的高效离体培养再生体系。结果表明,下胚轴和子叶均得到了高达100%的出愈率和95%以上的分化率;最佳的品种是T431;最佳激素组合为ZT1.0mg·L-1和IAA1.0mg·L-1。     

运用模糊数学和灰色系统理论分析评价夏播花生区试品种

运用模糊数学和灰色系统理论，对2007年河南省夏播花生区试品种进行了多因素综合评价和分析，结果表明：周花4号评价指标是0．7089，综合表现最优；其次是W9847—1—3—4—1，其综合评价指标是0．6352；豫花6号评价指标是0．3630，综合表现最差。此结果与试验地实际表现基本相一致。     

气象因子对棉花产量和品级影响的灰色关联分析

采用灰色关联分析法，研究了气候因子对棉花产量和纤维品级的影响。结果在明，气象因素的变化曲线与皮棉单产和纤维品级的变化曲线相似。气象因子与皮棉产量之间的灰色关联度平均为０．６４２２．与纤维品级之间的灰色关联度为０．５５５８．对湖南棉花皮棉单产和纤维品红影响最大的气候因子是降雨日和日照时数，影响最小的是日平均温度。     

试论灰色系统关联度分析法在生物科学研究中应用的合适性

灰色系统关联度分析法的主要研究对象是社会经济等非技术的抽象系统。近年来，在生物科学领域如畜牧、兽医、草原等技术性强和具体系统的科学研究中，发现有应用该法分析其试验数据的论文。经研究核实，应用该法分析生物科学研究的数据，似欠合适。这可能是由于该方法本身有不足处，或由于论文作者误用该法。特提出供读者与学者讨论与研究。     

哈达门国家森林公园游客量动态预测

根据哈达门国家森林公园的票房数据，应用灰色系统理论建立旅游量灰色动态模型。基此，对哈园游客量进行了中短期预测，其预测检验精度为９３．７２。旨在对该园建设、管理等项决策提供科学依据。     

罐藏番茄新品种红杂18的选育

红杂１８是以红２１３为母本，黄苗试材９３７５为父本，配制成适宜罐藏加工和长途运销的番茄一代杂种。具有高抗ＴＭＶ、中抗ＣＭＶ、果实硬度高、抗裂、耐压、耐贮运、果实加工性状优良等特点。已在新疆、甘肃、宁夏、广西、云南等省区的加工番茄生产基地和远运外销生产基地推广６４９．８ｈｍ２。     

Sensitivity of populations ofBotrytis cinerea to triazoles,benomyl and vinclozolin

Sensitivity of field isolates (121) ofBotrytis cinerea from France (1992), Germany (1979–1992), Israel (1990) and the Netherlands (1970–1989) to the triazoles tebuconazole and triadimenol, the benzimidazole benomyl and the dicarboximide vinclozolin were tested in radial growth experiments. Resistance to benomyl (in 21 to 100% of isolates tested) and vinclozolin (in 25 to 71% of isolates tested) was common in most countries. EC₅₀s (concentrations of fungicides inhibiting radial mycelial growth ofB. cinerea on B5-agar by 50%) for tebuconazole and triadimenol ranged between 0.01–1.64 and 0.4–32.6g ml^–1, respectively, and were log-normally distributed. The variation factor (ratio between EC₅₀s of the least and most sensitive isolate tested) amounts 164 and 82 for tebuconazole and triadimenol, respectively. These values are comparable to those for azole fungicides applied in control of other pathogens. Hence, variation in sensitivity to triazoles can probably not explain limited field performance of triazoles towardsB. cinerea. Isolates from south west Germany (1992) were significantly less sensitive to tebuconazole than isolates collected earlier in Germany, Israel and the Netherlands. Such less sensitive populations may contribute to the limited field performance of DMI fungicides towardsB. cinerea. The sensitivity of isolates from south west Germany to tebuconazole was similar to that of DMI-resistant mutants generated in the laboratory. These mutants displayed stable resistance with Q-values (ratio between EC₅₀ of resistant mutant and wild type isolate) between 5 and 20. Sensitivity of field isolates and laboratory mutants to tebuconazole and triadimenol was correlated.     

Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.     

温室番茄无土多层栽培技术开发研究

本研究的目的在于验证由山东农业大学园艺系研制的“鲁SC-1型”无土栽培装置及其配套栽培技术在大面积温室番茄无土多层栽培的有效性与可行性。番茄亩产量达12616.2公斤,亩产值超出土壤栽培184.98%,虽然生产成本比土壤栽培明显增大达138.34%。但由于增产基数超出土壤栽培较大,所以,亩净产值和盈利值分别比土壤栽培提高156.82%和227.73%。成本占用盈利率以及劳动盈利率等也均有明显超出。     

Age-related resistance to Pseudomonas syringae pv. tomato is associated with the transition to flowering in Arabidopsis and is effective against Peronospora parasitica

As plants mature it has been observed that some become more resistant to normally virulent pathogens. The ability to manifest the Age-Related Resistance (ARR) response in Arabidopsis to Pseudomonas syringae pathovars tomato (Pst) coincided with the transition to flowering in plants both delayed and accelerated in the transition to flowering. ARR was also associated with a change in PR-1 gene expression, such that young plants expressed PR-1 abundantly at 3 days post inoculation (dpi) while mature plants expressed much less. The Arabidopsis ARR response requires SA accumulation via isochorismate synthase (ICS1) [24]. ICS1 was expressed one dpi with virulent and avirulent Pst in both young and mature plants. The ARR response was also effective versus avirulent Pst providing an additional 4-fold limitation in bacterial growth. Arabidopsis ARR was found to be ineffective against two necrotrophs, Erwinia carotovora subspecies carotovora (bacterium) and Botrytis cinerea (fungus) and one obligate biotroph, Erysiphe cichoracearum (fungus). However, mature wild type, SA-deficient sid2 and NahG plants supported little growth of the obligate biotrophic oomycete, Peronospora parasitica. Therefore ARR to P. parasitica appears to be SA-independent, however the level of ARR resistance was somewhat reduced in these mutants in some experiments. Thus, there may be numerous defence pathways that contribute to adult plant resistance in Arabidopsis.