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Tomoko SUZUKI Takeshi SHINOGI Kazutoshi UNNO Yoshihiro NARUSAKA Pyoyun PARK 《Journal of General Plant Pathology》2002,68(4):267-276
This fluorescence and immunoelectron microscopic study showed that β-1,3-D-glucan accumulated only in leaves of a susceptible cultivar of Japanese pear after treatment with a host-specific toxin,
AK-toxin I, from Alternate, alternata Japanese pear pathotype. The positive fluorescent reaction of callose was detected only in aniline blue fluorochrome-stained
sections from toxin-treated leaves of the susceptible cultivar: positive sites were observed on cell walls of leaf cells.
The sites of callose deposition were probably consistent spatially with modified sites on the plasma membrane that were observed
only in the toxin-treated leaves of the susceptible cultivar. The toxin-induced modifications, identified as damage to the
plasma membrane, were characterized by invagination of the plasmalemma specifically at plasmodesmata and as the concomitant
accumulation of extracellular polysaccharides at the invaginated sites. A positive reaction to anti-β-1,3-D-glucan antibody was detected at the polysaccharides, Golgi vesicles, and trans-Golgi network (TGN) of toxin-treated leaves of the susceptible cultivar, but not at Golgi vesicles and TGN of water-treated
ones. The cis-, medial and trans-Golgi stacks of toxin-treated leaves of the susceptible cultivar were negative for the antibody. The results showed that
the polysaccharides, Golgi vesicles and TGN contained abundant β-1,3-D-glucan and that the glucan was transported from the Golgi apparatus via Golgi vesicles to the modified sites in cells of toxin-treated leaves of the susceptible cultivar.
Received 7 March 2002/ Accepted in revised form 10 June 2002 相似文献
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R. G. Milne Eliana Ramasso R. Lenzi Vera Masenga Nonglak Sarindu M. F. Clark 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(1):57-67
Methods are described for pre- and post-embedding immunogold labeling of mycoplasmalike organisms (MLOs) in thin sections of infected plants. Antisera against primula yellows (PY), tomato big bud (TBB) and bermudagrass white leaf (BGWL) MLOs, and a monoclonal antibody (mab) against PY were tested with the three serologically unrelated MLOs. Labeling was specific for each MLO and was localized to the outer surface of the MLOs. The antisera performed well in both pre- and post-embedding experiments; the mab reacted well in pre-embedding conditions but gave no labeling with post-embedding. Glutaraldehyde fixation reduced levels of labeling in post-embedding conditions. The results show that these techniques can be used to differentiate MLOs reliably, and extend the usefulness of electron microscopy in this area. 相似文献
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