应用基因芯片检测烟草脆裂病毒

 Total RNA in tulips was extracted by Trizol method. Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant. The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP. The probes of plant virus, 18S rRNA gene and comparisons were designed and immobilized on chips. Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software. Tobacco rattle virus was detected from tulips which were imported from Holand. The accuracy and sensitivity of the plant virus gene chip were proved.     

检疫性蛾类鉴定方法和标准的研究进展

由于检疫性蛾类多数种类个体小，形态难以观察，且与其他蛾类害虫在形态上具有一定的相似性，区别和鉴别难度大，造成该类检疫害虫传播扩散的风险加剧。一方面，该类害虫的幼虫啃食水果、木材等，使其品质下降，严重影响水果、木材等进出口贸易；另一方面，检疫害虫的扩散也对农林业生产构成了巨大威胁。因此，蛾类害虫的精准鉴定已成为国内外普遍关注的问题。该文对我国检疫性蛾类的种类进行系统性汇总，并对鉴定方法及鉴定标准等方面的研究进展进行综述，分析目前存在的问题和不足，并结合技术创新以及口岸实际检疫需求，提出今后的发展方向，以期为检疫性蛾类新型精准鉴定技术研发及其入侵防控策略制订提供参考。