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排序方式: 共有6166条查询结果,搜索用时 31 毫秒
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Ting Shi Yan-Yan Yu Jia-Jia Dai Yi-Ting Zhang Wen-Peng Hu Li Zheng Da-Yong Shi 《Marine drugs》2021,19(3)
The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi. 相似文献
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Fa-chao SUN Min TAN Yuan-chao ZHANG Yu-chao WANG Sheng-liang CAO Guo-fei DING Fang-yuan CONG Li-hong GUO Si-dang LIU Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442
To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV. 相似文献
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分子间的作用及其结构适应性 总被引:3,自引:0,他引:3
郭世豪 《湖南农业大学学报(自然科学版)》2001,27(2):148-150
某些分子间的作用无法的用范德华力、氢键、介电常数、偶极矩等化学基本理论来加以解释,引入控制论的方法对这些分析间的作用进行研究后的结果表明,选择性分析间引力很可能是分子是最强的物理作用,而分子间的化学作用亦与分子间的结构适应性密切相关。 相似文献
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分子标记用于赤眼蜂分子监测的研究 总被引:5,自引:0,他引:5
通过对松毛虫赤眼蜂(Trichogramma dedrolimi Matsumura)以及玉米螟赤眼蜂(Trichogramma ostriniae Pang et Chen)rDNA-ITS2基因的克隆测序,并联机检索GenBank核酸序列库中其它赤眼蜂的相关序列,利用核酸分析软件找到松毛虫赤眼蜂和玉米螟赤眼蜂ITS2序列中一段有鉴别意义的标志,并据此设计出蜂种的特异PCR引物,实现了松毛虫赤眼蜂和玉米螟赤眼蜂rDNA特异区带的PCR扩增。研究表明该特异区带具有种的特异性。我们认为该分子标记技术可用于目前我国生防中常用的松毛虫赤眼蜂和玉米螟蒌眼蜂的分子监测,如田间种群动态监控和寄生效果评估。 相似文献
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用RAPD技术筛选中国荷斯坦牛产奶量性状遗传标记 总被引:10,自引:3,他引:7
选用320条10碱基随机引物在由36头高产(305d产奶量〉8500kg)和32头低产(305d产奶量〈5600kg)中国荷斯坦牛所组成的2个DNA池间进行RAPD-PCR扩增,从中筛选出稳定性好、带型清晰且在2个DNA池间有明显差异的RAPD引物24条。用筛选到的24条引物对所有个体进行PCR检测,得到了5个与奶牛产奶量性状相关的RAPD标记,并对其中4个进行了克隆测序和SCAR标记转化以及生物信息学分析。结果表明:SCAR标记yield—s139与产奶量密切相关,应用电子克隆方法已将该标记由921bp延伸到2141bp,经同源性比较发现,此标记对应于奶牛基因组中LINEs家族中的一个重复元件,推测该元件附近可能存在与产奶量性状相关的QTLs或主效基因。 相似文献
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Studies of Various Diagnostic Methods for Canine Hypothyroidism 总被引:1,自引:0,他引:1
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Detection of the Defoliating Pathotype of Verticillium dahliae in Infected Olive Plants by Nested PCR 总被引:2,自引:0,他引:2
Jesús Mercado-Blanco Dolores Rodríguez-Jurado Encarnación Pérez-Artés Rafael M. Jiménez-Díaz 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(1):1-13
Spread of Verticillium wilt into newly established olive orchards in Andalucía, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to new areas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPD marker of the D pathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants. 相似文献