基于生物催化法的食品熏蒸剂环氧乙烷制备

为了探索一种反应条件温和、方法简单的食品熏蒸剂环氧乙烷制备方法,采用甲基弯菌IMV 3011(Methylosinus trichosporium IMV 3011)细胞作为生物催化剂,催化乙烯的环氧化反应生成环氧乙烷,确定了反应条件。结果表明:反应器中气相的组成为氧气50%、乙烯20%、氮气30%(体积分数),30℃、150 r/min振荡反应8 h,采用固定化形式催化剂,环氧乙烷生成量为34μmol/mg,利用甲烷培养对其循环再生,再生8次催化剂中甲烷单加氧酶(MMO)活力仍保留89%,环氧乙烷物质的量为3.4 nmol。     

Effect of temperature,organic amendment rate and moisture content on the degradation of 1,3‐dichloropropene in soil

1,3‐Dichloropropene (1,3‐D), which consists of two isomers, (Z)‐ and (E)‐1,3‐D, is considered to be a viable alternative to methyl bromide, but atmospheric emission of 1,3‐D is often associated with deterioration of air quality. To minimize environmental impacts of 1,3‐D, emission control strategies are in need of investigation. One approach to reduce 1,3‐D emissions is to accelerate its degradation by incorporating organic amendments into the soil surface. In this study, we investigated the ability of four organic amendments to enhance the rate of degradation of (Z)‐ and (E)‐1,3‐D in a sandy loam soil. Degradation of (Z)‐ and (E)‐1,3‐D was well described by first‐order kinetics, and rates of degradation for the two isomers were similar. Composted steer manure (SM) was the most reactive of the organic amendments tested. The half‐life of both the (Z)‐ and (E)‐isomers in unamended soil at 20 °C was 6.3 days; those in 5% SM‐amended soil were 1.8 and 1.9 days, respectively. At 40 °C, the half‐life of both isomers in 5% SM‐amended soil was 0.5 day. Activation energy values for amended soil at 2, 5 and 10% SM were 56.5, 53.4 and 64.5 kJ mol^?1, respectively. At 20 °C, the contribution of degradation from biological mechanisms was largest in soil amended with SM, but chemical mechanisms still accounted for more than 58% of the (Z)‐ and (E)‐1,3‐D degradation. The effect of temperature and amendment rate upon degradation should be considered when describing the fate and transport of 1,3‐D isomers in soil. Use of organic soil amendments appears to be a promising method to enhance fumigant degradation and reduce volatile emissions. Published in 2001 for SCI by John Wiley & Sons, Ltd     

Contact and fumigant activities of constituents of Foeniculum vulgare fruit against three coleopteran stored‐product insects

The insecticidal activities of materials derived from the fruit of fennel, Foeniculum vulgare, against adults of Sitophilus oryzae, Callosobruchus chinensis and Lasioderma serricorne were examined using direct contact application and fumigation methods. The biologically active constituents of the Foeniculum fruits were characterized as the phenylpropenes (E)‐anethole and estragole, and the monoterpene (+)‐fenchone, by spectroscopic analysis. Responses varied with insect species, compound, dose and exposure time. In a filter paper diffusion test, estragole at 0.168 mg cm^?2 caused 91% mortality to S oryzae adults within 1 day after treatment (DAT), whereas (+)‐fenchone and (E)‐anethole gave over 90% mortality at 2 and 4 DAT, respectively. Against C chinensis adults, all test compounds revealed potent insecticidal activities at 0.021 mg cm^?2 at 2 DAT. Against L serricorne adults at 0.105 mg cm^?2, (E)‐anethole gave 100% mortality at 1 DAT, whereas 90 and 60% mortality at 4 DAT was achieved with estragole and (+)‐fenchone, respectively. In a fumigation test, the test compounds were much more effective against adults of S oryzae, C chinensis and L serricorne in closed cups than in open ones, indicating that the insecticidal activity of test compounds was largely attributable to fumigant action. As naturally occurring insect‐control agents, the F vulgare fruit‐derived materials described could be useful for managing field populations of S oryzae, C chinensis and L serricorne. © 2001 Society of Chemical Industry     

Toxicity of cyanogen to insects of stored grain

Range-finding studies on the toxicity of cyanogen to all stages of five species of stored product Coleoptera are reported. The species were Rhyzopertha dominica (F), Sitophilus granarius (L), Sitophilus Oryzae (L), Tribolium castaneum (Herbst), Tribolium confusum Jacquelin du Val and Ephestia cautella (Walker). Exposures for 24 h to cyanogen at 1.3 mg litre(-1) controlled all external stages. Control of internal stages of Sitophilus species was achieved by a 5-day exposure to initial concentrations between 13.7 and 27.4 mg litre(-1), whereas R. dominica was controlled at 13.7 mg litre(-1). Cyanogen showed similar toxicity to all tested external stages and, in this respect, was more similar to methyl bromide than to phosphine. Its toxicity to insects increased with both relative humidity and concentration of carbon dioxide. Cyanogen was toxic to insects whether applied as a gas or in an aqueous solution.     

Phosphine resistance, respiration rate and fitness consequences in stored-product insects

Resistance to fumigants has been frequently reported in insect pests of stored products and is one of the obstacles in controlling these pests. The authors studied phosphine resistance and its physiological basis in adult insects of 12 populations of Tribolium castaneum (Herbst) (Tenebrionidae), ten populations of Rhyzopertha dominica (F.) (Bostrichidae) and eight populations of Oryzaephilus surinamensis L. (Silvanidae) from Brazil, and the possible existence of fitness costs associated with phosphine resistance in the absence of this fumigant. The bioassays for the detection of phosphine resistance followed the FAO standard method. The production of carbon dioxide and the instantaneous rate of population increase (r(i)) of each population of each species were correlated with their resistance ratios at the LC(50). The resistance ratio at LC(50) in T. castaneum ranged from 1.0- to 186.2-fold, in R. dominica from 2.0- to 71.0-fold and in O. surinamensis from 1.9- to 32.2-fold. Ten populations of T. castaneum, nine populations of R. dominica and seven populations of O. surinamensis were resistant to phosphine. In all three species there was significant association (P < 0.05) between respiration rate and phosphine resistance. The populations with lower carbon dioxide production showed a higher resistance ratio, suggesting that the lower respiration rate is the physiological basis of phosphine resistance by reducing the fumigant uptake in the resistant insects. Conversely, populations with higher r(i) showed lower resistance ratios, which could indicate a lower rate of reproduction of the resistant populations compared with susceptible populations. Thus, management strategies based on the interruption of phosphine fumigation may result in reestablishment of susceptibility, and shows good potential for more effective management of phosphine-resistant populations.     

储藏物螨类熏蒸防治方法研究状况及进展

文章综述了熏蒸杀螨剂的研究历史，２０种熏蒸杀螨剂对各龄期螨的毒力，在熏蒸剂对螨的毒力测定方面各研究材料之不一致性，熏蒸杀螨剂近年来研究进展，并据此对螨类防治的研究进行了预测。     

不同土壤熏蒸剂对烤烟苗床杂草的药效试验

以烟草品种龙江911为供试材料,对适每地、维博亩和斯美地这3种不同土壤熏蒸剂防除烟草苗床杂草效果进行了试验。结果表明,适每地和斯美地这两种土壤熏蒸剂对苗床杂草均有较好的防除效果。综合防除效果及安全性等因素．以斯美地效果最理想,其次是适每地,均可在黑龙江省烟区推广使用。     

甲酸乙酯的施药高度对仓内空间杀虫效果的影响

在储粮容量7000t的高大平房仓空仓内,设置一个高7.50 m、直径300mm的熏蒸室,分别在7.50m、5.61m、3.74m、1.87m和0.1m的高度放置试虫,先后从0.10m、3.74m和7.50m的高度施药,测定了甲酸乙酯对处于不同高度的赤拟谷盗(Tribolium castaneum(Herbst))和谷蠹(Rhyzopertha dominica(Fabricius))成虫的杀虫效果。结果为:在0.10m高度施药,甲酸乙酯对于靠近施药点的试虫可完全致死,而在1.87m及其以上高度处的害虫死亡率均显著低于施药点的死亡率。在高度3.74m处施药,施药点及其以下两种害虫的死亡率均显著大于施药点以上的害虫死亡率,3.74m及其以下的谷蠹均完全死亡,赤拟谷盗的死亡率则出现了0.1m处100%,1.87m和3.74m处则分别只有96.7%和81%。经7.50m高度施药,0.10m高度处的害虫死亡率达100%,且害虫死亡率出现自下而上递减的结果。本实验表明,作为熏蒸剂的甲酸乙酯在空间被施用后,静态条件下向上扩散且有效杀虫的高度小于2m;在高空施药后主要扩散趋势为向下运动(下沉),对施药点以下的害虫效果较好,建...