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1.
鸡传染性法氏囊病间接ELISA诊断试剂盒的研制及初步应用 总被引:3,自引:0,他引:3
采用vero细胞增殖的IBDV抗原建立了间接ELISA,用于定量检测IBDV抗体。对该方法进行了标准化研究,确立了该方法的最佳工作条件。在此基础上研制了相应的诊断试剂盒,并对盒内各组分的性状、保存、质量控制以及诊断试剂盒的特异性、敏感性、可重复性、符合率、与国外同类产品的比较、保存期等进行了全面、详细的研究。结果表明,研制的试剂盒在-20℃保存至6个月时各项性能都很好。该试剂盒与进口试刺盒对同样血清样品检测,符合率为86.7%。间接ELISA试剂盒与琼扩试验的符合率为92,5%。该试刺盒可对大量血清样品进行检测,操作简单方便、结果特异性强、敏感性高、快速,更适合于大型鸡场IBD免疫抗体水平的监测及现地疫病诊断等需要。 相似文献
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The influence of rain and drought before, and air temperature during, weed control with hot water was studied in laboratory experiments on the test weed Sinapis alba (white mustard). The plants were grown in a greenhouse and treated outdoors. There was no difference in weed control effect when S. alba plants at the four‐leaf stage were treated at the air temperatures 7°C and 18°C. The effective energy dose for a 90% fresh weight reduction was 465 kJ m?2 for both air temperatures. Weed control of S. alba at the four‐ to six‐leaf stage in rainfall above the rainwater run‐off level increased the required effective energy dose by 20% (i.e. 120 kJ m?2) compared with dry plants. A short period of drought just before treatment on S. alba at the two‐ to four‐leaf stage increased the plant fresh weight reduction, which was 22% at low energy dose (190 kJ m?2) and 44% at high energy dose (360 kJ m?2). Hot water weed control should thus be carried out when the plants are drought stressed and avoided when the plants are wet. The air temperature seems to be of little importance in the range 7–18°C. 相似文献
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N. D. Paveley † J. M. Thomas T. B. Vaughan N. D. Havis D. R. Jones 《Plant pathology》2003,52(5):638-647
A function was derived to predict fungicide efficacy when more than one application of a single active ingredient is made to a crop, given parameters describing the dose–response curves of the component single-spray applications. In the function, a second application is considered to act on that proportion of the total pathogen population which was uncontrollable at the time of the first application (represented by the lower asymptote of the dose–response curve for the first treatment), plus any additional part of the population which survived the first application as a result of a finite dose being applied. Data to estimate the single-spray dose–response curve parameters and validate predictions of two-spray programme efficacy were obtained from separate subsets of treatments in four field experiments. A systemic fungicide spray was applied to wheat at a range of doses, at one or both of two times (t1 and t2), in all dose combinations. Observed values of the area under the disease progress curve (AUDPC) for septoria leaf blotch (Mycosphaerella graminicola) were used to construct response surfaces of dose at t1 by dose at t2 for each culm leaf layer. Parameters were estimated from single-spray and zero-dose treatment data only. The model predicted a high proportion (R2 = 71–95%) of the variation in efficacy of the two-spray programmes. AUDPC isobols showed that the dose required at t2 was inversely related to the dose at t1, but the slope of the relationship varied with the relative timings of t1 and t2 in relation to culm leaf emergence. Isobols were curved, so the effective dose – the total dose required to achieve a given level of disease suppression – was lower when administered as two applications. 相似文献
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东北地区春玉米临界氮浓度稀释曲线的建立和验证 总被引:3,自引:0,他引:3
过量施氮是目前玉米栽培中存在的普遍现象,基于临界氮浓度稀释曲线计算得出的氮营养指数是诊断氮营养丰缺的重要手段。基于东北地区4个生态点的试验数据,构建了东北地区春玉米临界氮稀释曲线,并在此基础上建立了氮营养指数模型和需氮量模型,结果表明,东北地区春玉米地上部临界氮浓度与生物量符合幂函数关系。利用独立试验资料对建立的临界氮浓度稀释曲线进行检验,发现基于临界氮浓度稀释模型计算的氮营养指数可以准确诊断玉米植株的氮营养状况,并计算出实时的氮素需求量。该研究建立的东北地区春玉米临界氮稀释模型可以为该地区春玉米的氮营养诊断和动态调控提供较好的理论和技术指导。 相似文献
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The Danish decision support system Crop Protection Online (CPO) optimises herbicide weed control. CPO recommends specific herbicide solutions to achieve a required level of control. The aim is to apply herbicides as little as possible but as much as necessary. CPOWeeds is a version of CPO adjusted to conditions in North-eastern Spain. The predicted efficacies and the yield obtained with CPOWeeds were validated in winter cereal field trials from 2010 to 2013. All CPOWeeds treatments were related to the efficacies obtained with standard herbicide treatments decided upon by local advisors. The predictions from CPOWeeds were compared to the actually achieved efficacies in the field trials for the nine weed species at different developmental stages and for 84.2% of the comparisons the obtained efficacies were equal to or higher than predicted. The average difference between predicted and observed efficacies was 2.35 percentage points. Yield was measured in three trials and the recommendations from CPOWeeds were maintaining yield. There were two situations where CPOWeeds were performing suboptimal. One is in the early weed growth stages, as the model is not yet prepared to account for water stress on root action herbicides applied at 10-11 BBCH. The second situation was in fields with a prior unidentified population of resistant Alopecurus myosuroides. For key species in winter cereals in Spain, such as Avena sterilis, Lolium rigidum and Papaver rhoeas, CPOWeeds achieved a satisfactory control level. It was concluded that the use of CPOWeeds allowed optimisation of the herbicide application with a very high robustness. The recommendations were satisfactorily for the conditions of the Northeast of Spain and have the potential to decrease the amount of applied herbicides by at least 30%. Therefore, it can be an important tool in Integrated Weed Management. 相似文献
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Foliar pathogens such as Zymoseptoria tritici and Puccinia striiformis causing septoria leaf blotch and yellow rust respectively can cause serious yield reduction in winter wheat production, and control of the diseases often requires several fungicide applications during the growing season. Control is typically carried out using a constant fungicide dose in the entire field although there may be large differences in crop development and biomass across the field. The objective of the study reported in this paper was to test whether the fungicide dose response curve controlling septoria leaf blotch and other foliar diseases in winter wheat was dependent on crop development and biomass level. If such a biomass dependent dose response was found it was further the purpose to evaluate the potential to optimize fungicide inputs in winter wheat crops applying a site-specific crop density dependent fungicide dose. The study was carried out investigating fungicide dose response controlling foliar diseases in winter wheat at three biomass densities obtained growing the crop at three nitrogen levels and using variable seed rates. Further the field experiments included three fungicide dose rates at each biomass level, an untreated control, and 75%, 50% and 33% of the recommended fungicide dose rate and the experiments were replicated for three years. Crop biomass had a significant influence on occurrence of septoria and yellow rust with greater disease severity at increasing crop biomass. In two of three years, the interaction of crop biomass and fungicide dose rate had a significant influence on disease severity indicating a biomass-dependent dose response. The interaction occurred in the two years with high yield potential in combination with severe disease attack. If the variation in crop density and biomass level obtained in the study is representative of the variation found cultivating winter wheat in heterogeneous fields, then there seems to be scope for optimizing fungicide input against foliar diseases site-specific adapting the dose according to crop density/biomass. 相似文献