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普通小麦辉县红-荆州黑麦异染色体系的选育及其梭条花叶病抗性鉴定 总被引:3,自引:1,他引:3
为发掘和利用荆州黑麦所携抗梭条花叶病基因,综合利用分子细胞遗传学与分子标记技术结合多年抗性鉴定,从高感梭条花叶病小麦地方品种辉县红与荆州黑麦杂交后代(F7~F9)中选育出二体异附加系5个(分别添加1R、2R、R3、5R和R7)、5RS端二体异附加系1个和多重异附加代换系2个(染色体组成分别为20’’+2R(2D)’’+4R’’和19’’+1R(1B)’’+2R(2B)’’+4R’’)。鉴定表明,双二倍体荆辉1号高抗梭条花叶病,表明黑麦抗性基因可在小麦背景中稳定表达,2R、R7二体异附加系及2个含2R的多重异附加代换系均表现高抗,推测2R和R7上可能携带抗病基因。这些材料是研究荆州黑麦抗性基因遗传及小麦抗病育种的新种质。 相似文献
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Molecular comparisons amongst wheat bymovirus isolates from Asia, North America and Europe 总被引:2,自引:0,他引:2
Jiong Chen Sohn Chen Lei Cheng Schulze Steinbiss Antoniw & Adams 《Plant pathology》1999,48(5):642-647
To study the variation between wheat bymovirus isolates and to resolve uncertainties about the identity of the virus in some countries, leaves of infected plants were obtained from nine sites in China and from one each in Italy, Germany, USA and Canada. The German isolate was obtained from rye and the Canadian isolate was the type strain of wheat spindle streak mosaic virus (WSSMV). In RT-PCR, using primers designed from a partial sequence of a French isolate (tentatively described as WSSMV), genome fragments were obtained from the Italian and the French isolates but not from the Chinese ones. Conversely, products were consistently obtained from the Chinese isolates, but not from the Italian or French ones, when primers were designed from the sequence of a Japanese isolate of wheat yellow mosaic virus (WYMV). Nucleotide sequences were obtained from regions at or near the 3'-terminus of RNA1 of six Chinese isolates and the four from Europe and North America, usually including the coat protein. Nucleotide and amino acid sequence comparisons demonstrated that the European and North American isolates were extremely similar and were therefore WSSMV, while the Chinese isolates were close to the Japanese isolate and were thus WYMV. 相似文献
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我国真菌传大小麦病毒病的地理分布 总被引:3,自引:0,他引:3
作者于1990~1995年调查我国大、小麦各生态区,共计20个省、市、区,116个县、市的大麦黄花叶病、小麦黄花叶病(小麦梭条斑花叶病)和小麦土传花叶病的分布区域。结果表明,3种真菌传病毒病其分布仅限于冬麦区。大麦黄花叶病主要集中分布在长江三角洲、钱塘江流域和东部沿海。小麦黄花叶病主要分布于长江中、下游及其支流大渡河、青衣江,黄河中、下游及其支流渭河流域、淮河流域。小麦土传花叶病仅局部冬麦区发生,与小麦黄花叶病混合并发。3种病害的分布范围为北纬28~37°50′东经102~122°40′之间。 相似文献
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G. E. Budge C. Ratti C. Rubies-Autonell D. Lockley M. Bonnefoy V. Vallega S. Pietravalle C. M. Henry 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(3):259-272
Twenty-one UK winter wheat cultivars were grown over three seasons at sites with natural inoculum sources of Soil-borne cereal mosaic virus (SBCMV) and Wheat spindle streak mosaic virus (WSSMV) located in France, Italy and the UK. Plants were assessed visually for virus symptoms and leaf extracts were tested
for the presence of each virus using enzyme-linked immunosorbent assays (ELISA). Cultivars showing little or no foliar symptoms
and low levels of virus in leaf tissue were classified as resistant to each virus. All the trials were taken to harvest and
agronomic data collected. At the most heavily infected sites, severe symptoms of SBCMV were observed in all UK cultivars except
Aardvark, Charger, Claire, Cockpit, Hereward and Xi 19. The latter cultivars exhibited either light or no symptoms and little
or no SBCMV infection in leaves. In fields with WSSMV, the virus failed to develop in Italy, but was detected in the leaves
of all the susceptible control cultivars at a site in France. However, no UK cultivar tested positive for WSSMV. Multi-site
analysis indicated that the presence of WSSMV did not increase the impact of SBCMV on the height, thousand-grain weight or
yield of UK cultivars. The wheat cultivars on test gave a similar response to SBCMV across three European countries. Possible
sources of SBCMV resistance are discussed. 相似文献
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