多酚类化合物协同抗氧化性研究进展

多酚类化合物是一种大量存在于植物体内的物质，因其具有优良的抗氧化、抗癌、抗衰老等 性能而受到研究者们的广泛关注。多项研究表明具有抗氧化性能的物质之间大多具有协同作用，两 种或两种以上的物质化合，其抗氧化能力往往强于单一物质的抗氧化能力。近年来，许多研究者将目 光聚焦到多酚类物质与小分子物质化合所具有的协同抗氧化能力的研究上并做出多项试验。本文论 述了多酚类化合物的抗氧化机理及协同抗氧化机理，并以茶多酚与维生素C、维生素E 化合为例对协 同抗氧化进行论述。     

维生素E与母牛繁殖

维生素E是一种有效的抗氧化剂，具有广乏的生理功能，对提高机体免疫力，提高母牛繁殖力和预防产科疾病有重要的意义。本文从它的生理功能、来源及与母牛繁殖关系的最新研究成果进行了综述。     

串励直流电动机使用无触点可控硅斩波调速的研究

本文提出了串励直流电动机使用新型无触点斩波调速的新方法。通过两组二段式斩波电路,主电路完全无触点化成为可能,而且扩大了调速使用范围,也证明了可应用于双机车牵引同步自动化控制。     

遗传背景对水稻无融合生殖材料C1001双胚苗频率影响的研究

同核异质雄性不育材料与无融合生殖材料Ｃ１００１Ｂ杂交和回交表明，Ｃ１００１Ｂ的双胚苗，是受核基因控制的性状，双胚苗频率的表达，具明显的胞质效应和胞核效应，在胞质效应中，不育胞质与可质胞质的差异尤其显著，不育胞质对双胚苗的表现有明显的抑制作用，不同世代间胞质效应和胞核效应的表现也不一致。此外，环境条件对Ｃ１００１的双胚苗表现有一定的影响。     

C肉毒杀鼠素在高寒草甸地区灭鼠技术中的应用

综述C肉毒杀鼠素草原灭鼠应用技术,充分肯定了C肉毒杀鼠素毒力强、适口性好、对非靶动物毒性低、作用缓慢、无二次中毒、易降解和适合规模灭鼠的特点,为鼠害防治提供了新的思路。     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

台湾樟芝对碳素营养源利用的研究

本文报道了台湾樟芝 (Antradiacomphora)液体深层培养过程中 ,六种不同碳素营养源组成的合成液体培养基 ,对樟芝菌球生长的影响 ,结果表明 ,樟芝对六种碳源均可利用 ,其中以麦芽糖利用效果最佳 ,菌球数量及菌丝体干重均居首位 ,菌球数量是对照的 3 5 5倍 ,菌丝体干重比对照高出1 73倍 ,樟芝对乳糖的利用较差 ,菌球数量虽比CK仅少 2 5个 / 10 0mL ,但菌体干重相差较大 ,每10 0mL相差 2 5 6 9mg。对樟芝发酵液pH测定情况看来 ,不同碳源组成的培养基终止pH表现不同 ,以麦芽糖、葡萄糖、果糖、蔗糖为碳源的培养基终止pH为 4 5～ 5 0左右。甘露醇为碳源的培养基终止发酵pH为 3 5～ 4 0 ,而乳糖则为 5 5以上。试验表明 ,樟芝菌丝生长极其缓慢 ,在液体深层培养过程中 ,若培养基配方不合理 ,菌球就不生长 ,本文筛选了适合樟芝深层培养的碳素营养源 ,为樟芝的开发和利用提供了科学依据     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

维生素E对绵羊鲜精及冻精精液品质的影响

日粮中添加维生素E可以提高绵羊鲜精的活率 ,对照组和试验组的活率分别为 0 72± 0 0 7和 0 78± 0 0 6(P <0 0 5 ) ,显著改善鲜精精液品质。采用两步稀释法在绵羊冷冻精液稀释液中添加维生素E ,可以极显著降低冷冻对精子顶体的冷刺激损害程度 ,试验组的活率 ( 0 43 5± 0 0 2 0 )极显著高于对照组 ( 0 3 65± 0 0 2 6) (P <0 0 1) ,精子顶体的总异常率试验组 ( 3 3 72 % )极显著低于对照组 ( 4 4 3 5 % ) (P <0 0 1) ,其中试验组顶体膨胀率和脱落率与对照组分别为 1 2 8%±0 5 8%、2 3 0 8%± 1 45 %与 2 68%± 0 5 9%、2 8 96%± 3 14 %。冷冻精液品质显著改善。