不同密度对露草地被的影响

露草作为一种温室藤蔓花卉通常作室内吊挂及盆栽摆放应用,通过对地被栽培的露草生态习性、生长状况、生长量调查与分析,初步探索出其地被应用的可行性及适宜定植密度,使其四季常青的枝叶、美丽的花朵、良好的匍匐性及快速生长铺地能力等特性得以发挥。     

Influence of feeding Tinospora cordifolia peripartum on lactation parameters in crossbred cows

For studying the effect of dietary supplementation of guduchi (Tinospora cordifolia) peripartum on lactation an investigation was conducted on 15 pregnant Karan Fries crossbred cows which were divided into two groups: treatment group of eight cows which were supplemented with guduchi at 60 g/day for 45 days prepartum and 120 g/day for 45 days postpartum; control group of seven pregnant cows which were not supplemented with guduchi. Jugular blood samples were collected from all cows during the periparturient period for analysis of various blood cell and plasma parameters. A significantly higher total leukocyte count, lymphocyte count, neutrophil count and neutrophil/lymphocyte ratio was recorded in the guduchi supplemented treatment group in comparison to untreated control cows throughout the experimental period. The increase of milk production over 305 days of lactation due to guduchi supplementation was significant (p < 0.05). A significant (p < 0.05) reduction in somatic cell count was also observed during the experimental period. Milk composition (fat, protein, lactose and SNF) was similar (p > 0.05) for both the groups. Plasma non esterified fatty acid (NEFA) concentrations were significantly higher (p < 0.01) in cows supplemented with guduchi throughout the course of study. Plasma concentration of growth hormone in the treated cows was also significantly higher beginning on the day of parturition up to 3 weeks postpartum (p < 0.05) in comparison to unsupplemented group.     

茜草不同极性提取物的体外抑菌活性研究

本文采用k-b纸片法研究了茜草不同极性溶剂提取物的抑菌作用,结果表明,石油醚提取物浓度为50mg／ml时,抑菌率为25．03％;乙酸乙酯层提取物浓度为50mg／mL时抑制率最高,为48．64％;丙酮层提取物浓度为50mg／mL时抑制率最高,为61．59％;甲醇层提取物浓度为50mg／mL时抑制率最高,为46．09％。     

肾蕨组培快速繁殖的研究

该文通过肾蕨走茎离体培养实验,研究走茎不同部位及不同激素浓度对ＧＧＢ（绿色球状体）诱导及器官发生的影响．结果表明,采用走茎茎尖作为外植体的ＧＧＢ诱导率高,分化增殖系数大,小植株生长快．产生ＧＧＢ和芽的分化分别以ＭＳ＋ＩＡＡ０．１ｍｇ·Ｌ－１＋ＮＡＡ０．５ｍｇ·Ｌ－１和ＭＳ＋ＩＡＡ０．５ｍｇ·Ｌ－１＋ＮＡＡ０．０５ｍｇ·Ｌ－１为好,根的诱导以ＭＳ＋ＮＡＡ０．１ｍｇ·Ｌ－１为佳．同时,对外植体的消毒提出了新方法,而较过去有所突破．最后,对有关肾蕨组培中的一些问题进行了讨论．     

Anthraquinone production by callus cultures of Rubia cordifolia

Munjistin and purpurin were identified as the major components of anthraquinone pigments produced by callus cultures of R. cordifolia. Anthraquinone content in calluses was 0.62–1.22% (by dry wt.) depending on the source of explants. Selection of coloured aggregates yielded a cell line with twofold increase in anthraquinone production.     

露草卡那霉素(Kanamycin)抗性筛选研究

露草种子卡那霉素(Km)抑制发芽试验结果显示,在含有Km的MS固体培养基上露草种子萌发苗芽长、主根长、侧根数明显减小,子叶颜色由绿转黄;培养皿浸种发芽,当Km浓度达到100 mg/L时,萌发苗子叶颜色为黄色.由此确立了卡那霉素筛选转基因露草种子的适宜方法.     

露草营养器官解剖结构

[目的]观察露草(Aptenia cordifolia)营养器官解剖结构,探讨其耐旱机理。[方法]采用石蜡切片法,在光学显微镜下观察露草根、茎、叶的解剖结构。[结果]根有一定的次生生长,产生周皮和次生维管组织,次生维管组织呈多轮分布。茎表皮单层细胞且外壁具角质层,皮层较厚且其中分布有散生维管束。叶为肥厚等面叶,上、下表皮分布有较密集的无规则型气孔器。[结论]露草根具周皮和次生维管组织,茎具角质层和厚的皮层、叶为等面叶等特征,与其抗旱性有关。露草的根、茎和叶中都分布有许多含针晶簇的细胞,茎、叶的表皮外均分布有许多盐囊泡,可能既是抗旱又是耐盐特征。     

茜草提取物对奶牛乳房炎主要病原菌的抑菌试验

[目的]明确茜草（Rubia cordifolia L．）乙醇提取物不同萃取部分的抑菌活性。[方法]选择金黄色葡萄球菌、停乳链球菌、无乳链球菌、大肠杆菌、变形杆菌和绿脓杆菌为代表菌种,用不同极性的溶剂对茜草根的乙醇提取物进行分步萃取,筛选出对奶牛乳房炎主要病原菌有较强抑制作用的萃取部分。[结果]茜草提取物的石油醚、氯仿、乙酸乙酯萃取部分具有抗菌活性。其中乙酸乙酯萃取部分对奶牛乳房炎5种病原菌的抑菌效果最强．氯仿与石油醚萃取部分作用相当,正丁醇萃取部分对6种病原菌不敏感。丙酮萃取部分无抑菌作用。     

Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina

The n-hexane-dichloromethane (1:1) extract of the roots of Onosma argentatum and the methanol extract (partitioned between water and chloroform, ethyl acetate and n-butanol, respectively), of the underground parts (roots and rhizomes) of Rubia peregrina were tested in vitro for their antioxidant and antimicrobial activities. The highest antioxidant activity (98%) was observed at 0.1% concentration for the roots of O. argentatum. It was 96% at 0.25% concentration on the ethyl acetate fraction of R. peregrina. O. argentatum extract was effective on Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The ethyl acetate and chloroform fractions of R. peregrina were effective on S. aureus and E. coli, respectively. These two species did not have any antifungal activity.     

茜草果实色素的提取方案初探

采用以水、乙醇、丙酮、乙酸乙酯、石油醚作为溶剂在相同的条件下对茜草果实色素进行提取研究 ,选最佳溶剂。然后在最佳溶剂不同条件下进行对比实验 ,结果表明 :以水作提取溶剂最好。并且在料比为 1∶3、提取温度为 60℃ ,提取时间为 75mins,提取次数为 2次时最佳。