全文获取类型
收费全文 | 1168篇 |
免费 | 25篇 |
国内免费 | 44篇 |
专业分类
林业 | 193篇 |
农学 | 154篇 |
基础科学 | 5篇 |
61篇 | |
综合类 | 498篇 |
农作物 | 15篇 |
畜牧兽医 | 7篇 |
园艺 | 245篇 |
植物保护 | 59篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 11篇 |
2021年 | 15篇 |
2020年 | 20篇 |
2019年 | 31篇 |
2018年 | 20篇 |
2017年 | 31篇 |
2016年 | 48篇 |
2015年 | 32篇 |
2014年 | 39篇 |
2013年 | 57篇 |
2012年 | 104篇 |
2011年 | 86篇 |
2010年 | 95篇 |
2009年 | 75篇 |
2008年 | 73篇 |
2007年 | 97篇 |
2006年 | 55篇 |
2005年 | 52篇 |
2004年 | 63篇 |
2003年 | 31篇 |
2002年 | 26篇 |
2001年 | 24篇 |
2000年 | 17篇 |
1999年 | 31篇 |
1998年 | 20篇 |
1997年 | 8篇 |
1996年 | 12篇 |
1995年 | 3篇 |
1994年 | 13篇 |
1993年 | 8篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有1237条查询结果,搜索用时 8 毫秒
1.
Roger Voisin Maria-Jose Rubio-Cabetas Jean-Claude Minot Daniel Esmenjaud 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(1):103-108
Penetration, development and emigration of M. arenaria in the roots of three Myrobalan plum (Prunus cerasifera) clones genetically characterized for their resistance to root-knot nematodes (RKN) were studied during the 10 (penetration) and 15 (emigration) days following the date of inoculation (D) of 2500 juveniles (J2s) per plant into the soil. Miniaturized tests were conducted on the two resistant clones P.2175 (Ma1 gene) and P.1079 (Ma2 gene) and the susceptible clone P.2032 (recessive for both genes), obtained from micropropagated plantlets and grown in mini-containers under controlled conditions at 25°C in a growth chamber. For penetration and development studies, nematodes in the roots were recovered by the acid fuchsin-lactophenol staining technique. Equivalent numbers of J2s were recovered in all the clones at D+1 and D+2. Subsequently, the numbers increased rapidly in P.2032 and were significantly different from those in P.1079 and P.2175 that remained at a low level. No swollen larvae were observed in the resistant clones. In P.2032, the first swollen larvae were observed at D+4, the first females were observed at D+12, whereas the first females with attached egg sacs and the first new-generation J2s were obtained between D+21 and D+28. Our data suggest that the resistance phenomenon does not act on the very early nematode penetration but acts later by preventing feeding-site induction and development into the third-stage. For emigration studies, plants in which J2s had been allowed to penetrate for two days (from D to D+2) were washed free of soil, repotted and then, after various periods of growth, soil-free roots were placed under a mistifier to evaluate the numbers of emigrating individuals. Emigration of J2s from the roots occured mainly from D+2 to D+4 in all the genotypes and was very limited from D+4 to D+10. There was no significant differences in the number of emigrated juveniles between the resistant and susceptible clones, indicating that emigration cannot explain the difference in the numbers of nematodes recovered in the roots. 相似文献
2.
Diversity of the coat protein-coding region among Ilarvirus isolates infecting hop in Australia 总被引:1,自引:0,他引:1
D. R. Crowle S. J. Pethybridge G. W. Leggett L. J. Sherriff C. R. Wilson † 《Plant pathology》2003,52(5):655-662
Coat protein (CP) sequences of 17 Ilarvirus isolates were obtained from hops at three farms in Tasmania, Australia. Phylogenetic analysis of these sequences and additional database sequences indicated several Apple mosaic virus (ApMV) isolate clusters distinct from Prunus necrotic ringspot virus (PNRSV): one containing isolates from apple; one containing a single isolate from almond; a third containing Australian hop isolates of the 'apple' serotype and a German isolate of unknown origin; and a fourth containing Australian hop isolates of the 'intermediate' serotype. Isolates from hop, pear and prune from the Czech Republic either formed a fifth grouping, or were divergent members of the 'intermediate' serotype group. Deduced amino acid (aa) residue differences between the coat proteins of the two hop isolate serotype groups were highlighted as possible regions of serological differentiation. No evidence for coinfection of plants with both serotypes was found. Tests of ApMV-infected hop buds using the Shirofugen flowering cherry assay revealed a possible differentiation of the two strains based on hypersensitivity. Because of serological similarities to PNRSV, these viruses have commonly been reported as strains of PNRSV. However, this study shows ilarviruses from Australian hops are strains of ApMV, but distinct from those infecting Malus spp. 相似文献
3.
Lúcia PEREIRA Ana VASQUES Paula MAIA Maria Joo RAMOS PEREIRA Carlos FONSECA Milena MATOS 《Integrative zoology》2019,14(3):280-292
The stone marten (Martes foina) is a carnivorous mammal that often consumes fleshy fruits, thus potentially promoting seed dispersion. The present study was developed in Bussaco National Forest, central Portugal, and aimed to assess the potential role of the stone marten in dispersing native and exotic plants in different forest landscape types. Seeds from stone marten scats and fleshy fruits were collected monthly and were thereafter identified and sowed in a nursery, following a randomized experimental setup. Plant emergence was monitored fortnightly. Generalized linear models were used to test for differences in time and success of emergence between seeds from scats and fruits of 3 native species (Rubus ulmifolius, Arbutus unedo and Celtis australis) and 1 exotic plant species with invasive behavior (Prunus laurocerasus). Fruit consumption by the stone martens significantly increased and accelerated the germination of the native R. ulmifolius but had no effect on the other 2 native species or on the invasive species. This suggested that stone martens contribute to gene flow and forest regeneration by dispersing native plant seeds. However, although the germination was not enhanced in the invasive species, the preference of stone martens for these fruits may potentially contribute to the proliferation of P. laurocerasus. Our study represents a contribution to better understanding the fauna and flora interactions, enabling for a more conscious and effective decision‐making in forest management. 相似文献
4.
5.
为了解榆叶梅(Prumus triloba Lindl.)在短期碱胁迫下的分子响应机制,本研究采用Illumina HiSeqTM2500高通量测序技术对其在0、1、3、6、12 h碱胁迫下的叶片进行了de novo转录组测序,以此鉴定与碱胁迫相关的差异表达基因(DEGs)。结果表明,从5 960万条raw reads中获得了约5 300万条高质量clean reads,经从头组装后获得了124 786条榆叶梅unigenes。基于8 948个unigenes差异表达(DEGs), GO富集分析发现,其中有28个基因可能在早期碱胁迫响应机制中起着重要作用; KEGG富集分析发现,不同碱处理时间下的KEGG途径(pathways)具有显著差异。对7个碱性胁迫反应相关基因进行RT-qPCR验证,所得基因的表达模式与RNA-Seq数据结果一致,证实了RNA-Seq结果的可靠性。本研究首次对碱胁迫下的榆叶梅叶片进行了转录组分析,获得的序列数据极大地丰富了榆叶梅可利用的基因资源,深入了解了榆叶梅碱胁迫的分子响应机制,同时为研究以榆叶梅为砧木的中国李(Prunus salic... 相似文献
6.
7.
8.
水杨酸对采后桃果实脂氧合酶及相关指标的影响 总被引:5,自引:0,他引:5
水杨酸 (salicylicacid ,SA)是一种植物体内产生的简单酚类化合物 ,广泛存在于高等植物中。已发现 ,外源施用SA可诱导某些植物开花和产热 ,诱导烟草和黄瓜等植物对病毒、真菌及细菌等病害的抗性 ,Raskin( 1 992 )提出SA可以被看作是一种新的植物内源激素 ,其作用和机制受到人们的关注。外源SA处理对园艺产品采后的作用研究还较少 ,仍有许多问题尚待探讨。本试验以 2个不同品种的桃果实为试材 ,研究了SA处理后果肉组织脂氧合酶活性以及其他相关指标的变化 ,并测定了果肉的硬度 ,为进一步探讨桃果实采后成熟衰老机制 ,改进现有贮藏方式提… 相似文献
9.
不同药剂浸泡处理对山桃稠李种子发芽的影响 总被引:1,自引:0,他引:1
为了促进山桃稠李种子萌发,提高山桃稠李种子繁殖的发芽率,以清水作为对照,采用不同浓度的高锰酸钾、过氧化氢、赤霉素对山桃稠李种子进行处理,比较不同药剂、不同浓度及不同处理时间对山桃稠李种子发芽的影响。结果表明:不同药剂处理的种子发芽率有显著差异,不同处理时间之间没有显著差异,各药剂处理按照发芽率由高到低的顺序排列依次为高锰酸钾、赤霉素、过氧化氢、水处理。对发芽促进效果最佳的组合是0.7%的高锰酸钾浸泡处理48h,发芽率可达88%。 相似文献
10.
Michelle T. Hulin Robert W. Jackson Richard J. Harrison John W. Mansfield 《Plant pathology》2020,69(6):962-978
Bacterial canker disease is a major limiting factor in the growing of cherry and other Prunus species worldwide. At least five distinct clades within the bacterial species complex Pseudomonas syringae are known to be causal agents of the disease. The different pathogens commonly coexist in the field. Reducing canker is a challenging prospect as the efficacy of chemical controls and host resistance may vary against each of the diverse clades involved. Genomic analysis has revealed that the pathogens use a variable repertoire of virulence factors to cause the disease. Significantly, strains of P. syringae pv. syringae possess more genes for toxin biosynthesis and fewer encoding type III effector proteins. There is also a shared pool of key effector genes present on mobile elements such as plasmids and prophages that may have roles in virulence. By contrast, there is evidence that absence or truncation of certain effector genes, such as hopAB, is characteristic of cherry pathogens. Here we highlight how recent research, underpinned by the earlier epidemiological studies, is allowing significant progress in our understanding of the canker pathogens. This fundamental knowledge, combined with emerging insights into host genetics, provides the groundwork for development of precise control measures and informed approaches to breed for disease resistance. 相似文献