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甘薯膜脂过氧化作用和膜保护系统的变化与品种抗旱性的关系 总被引:16,自引:1,他引:16
对水分胁迫下甘薯叶片膜脂过氧化作用和膜保护系统变化的研究结果表明 ,水分胁迫下不同甘薯品种叶片中O2-·产生速率、MDA含量、SOD和POD活性、VC 含量比对照均有明显增加 ;除少数抗旱性较强的品种外 ,其余品种CAT活性比对照均有不同程度下降 ,但品种抗旱性愈强 ,其CAT活性下降幅度愈小。O2-· 和MDA的相对值(占对照 % )与品种抗旱性均呈极显著负相关 (r分别为 - 0 .772 8和 - 0 .836 2 ,P <0 .0 1) ,而SOD、POD、CAT及VC 的相对值与品种抗旱性均呈极显著正相关 (r分别为 0 .95 19、0 .82 2 6、0 .96 13和 0 .92 30 ,P <0 .0 1)。因此 ,这些指标可用于甘薯不同品种抗旱性的评定 相似文献
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Hansen RA Savage CJ Reidlinger K Traub-Dargatz JL Ogilvie GK Mitchell D Fettman MJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(4):457-463
An 18-week feeding trial was performed to investigate the effects of an omega-3 (n-3) fatty acid-enriched ration on plasma fatty acid concentrations and platelet aggregation in healthy horses. Flaxseed oil served as the source of the n-3 fatty acid alpha-linolenic acid (ALA). Twelve horses were fed dietary maintenance requirements using a complete pelleted ration (80%) and timothy grass hay (20%) for a 2-week acclimation period before being randomly assigned either to a treatment (group 1) or control (group 2) group. Group 2 horses (n = 6) were fed the diet described in the acclimation period, whereas group I horses (n = 6) were fed a 10% flaxseed oil-enriched complete pellet (80%) and grass hay (20%). Biological samples and physical measurements were collected at one point during the acclimation period (week 0) and every 4 weeks thereafter (weeks 4, 8, 12, and 16). Body weight, CBC (including platelet count), plasma fibrinogen. electrolyte (Na, K, and Cl) concentrations, and biochemical profile enzyme activities (aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltransferase, and creatine kinase) did not change markedly with diet. Platelet aggregation was not altered by the supplementation of flaxseed oil in these healthy horses, although increases in plasma cis-polyunsaturated 18-carbon fatty acids C18:3; n-3 (ALA) and C18:2; n-6 (linoleic acid), biologically active C20:5; n-3 (eicosapentaenoic acid [EPA]), and malondialdehyde (MDA) were evident. There were no marked decreases in C20:4; n-6 (arachidonic acid [AA]) or increases in C22:6; n-3 (docosahexaenoic acid [DHA]), signifying that flaxseed oil may have had a high percentage of omega-6 (n-6) fatty acids as well as n-3 fatty acids, and this relatively high n-6: n-3 fatty acid ratio may have affected the biochemical effect of n-3 fatty acids. In healthy horses supplemented with flaxseed oil, platelet aggregation was not altered, which may have been due to the limited biologic effect in healthy subjects or the inability of flaxseed oil to induce the necessary biochemical effect of replacing n-6 fatty acids with n-3 types. 相似文献
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Phytotoxic activity of middle-chain fatty acids II: peroxidation and membrane effects 总被引:1,自引:0,他引:1
Barbara Lederer Takane Fujimori Yasuko Tsujino Ko Wakabayashi Peter Bger 《Pesticide biochemistry and physiology》2004,80(3):151-156
Pelargonic acid (PA), an aliphatic 9-carbon monocarboxylic acid, is a phytotoxic burn-down compound. In the light peroxidizing activity can be measured as ethane and propane formation with cress or tobacco seedlings. This effect is strong at low pH (4–5), and saturated acids with 9–10 carbon atoms represent the optimum chain length. Methyl or ethyl esters are inactive, and safeners have no influence. In contrast to the peroxidative herbicides like acifluorfen methyl neither photosynthesis nor protoporphyrin IX is involved, although peroxidation requires light. Chlorophyll is necessary since etiolated seedlings show little peroxidation. Singlet oxygen quenchers like eugenol markedly reduce peroxidation. Membrane leakage of a similar rate is observed in light as well as in darkness. PA was described as a penetration enhancer intercalating with membranes. Our data corroborate that conclusion. Accordingly, the herbicidal mode of action of pelargonic acid is due first to membrane leakage in dark or light and second to peroxidation driven by radicals originating in the light by sensitized chlorophyll displaced from the thylakoids. 相似文献
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Mehmet Be?ir Akpinar Semsettin Sahin Atilla Ilhan 《Pesticide biochemistry and physiology》2005,82(3):233-239
Rotenone, an insecticide, causes toxicity through inhibition of mitochondrial electron transport chain at complex I and oxidative injury to the tissues. The aim of the present study was to determine in vivo effects of rotenone on myocardium and cardio-protective effects of caffeic acid phenethyl ester (CAPE), an antioxidant agent, against rotenone toxicity in rats. The rats were divided into three groups: untreated control, rotenone (2.5 mg/kg/day for 60 days, i.p.) and rotenone + CAPE groups. CAPE was administrated i.p. 10 μmol/kg/day for 62 days started two days before first dose rotenone injection. The malondialdehyde, nitric oxide levels and xanthine oxidase activity of rotenone group was significantly higher than control and rotenone + CAPE groups (p < 0.05). However, catalase activity in the rotenone group was decreased in comparison with the other groups (p < 0.05). The superoxide dismutase activity of rotenone group was insignificantly decreased compared to the others. In conclusion, rotenone caused lipid peroxidation in myocardial tissue and CAPE treatment prevented this rotenone-induced lipid peroxidation in rats. CAPE might be a cardio-protective agent against myocardial toxicities. 相似文献
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低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响 总被引:22,自引:1,他引:22
为了给小麦抗寒性鉴定提供依据,采用室内模拟低温处理小麦133个系的DH群体幼苗,研究了低温胁迫对小麦幼苗叶片脂质过氧化作用的产物及其相关酶活性的影响。结果表明,低温处理后脂质过氧比作用产物丙二醛(MDA)的含量增加;超氧物歧化酶(SOD)和过氧化物酶(POD)的活性在低温胁迫条件下不同株系间存在差异,但并不随MDA的增加而稳定提高,两种酶之间的活性变化无明显的同步性。因此,SOD活性和MDA含量可以作为鉴定小麦抗寒性的生理生化指标。 相似文献
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紫外光B辐射增强对小白菜膜脂质过氧化作用的影响 总被引:5,自引:2,他引:5
小白菜品种矮脚黄生长在光照培养室内,在以0.0、13.0kJm^-2d^-1(模拟氧浓度下降20%时的UV-B强度)的紫外光B(UV-B280-320nm)进行4d与7d的照射处理,研究UV-B对小白菜叶片内的类黄酮和膜脂质过氧化作用的影响。研究结果表明,UV-B辐射诱导了小白菜叶内吸收UV的类黄酮化合物的积累。同时UV-B辐射降低小白菜叶片内抗坏血酸的含量,13.0kJm^-2d^-1的UV-B 相似文献
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PEG渗调对苦瓜种子活力和膜脂过氧化的影响 总被引:20,自引:4,他引:20
15% ̄35%PEG能提高苦瓜种子活力,但以25%PEG渗调效果最好,经25%PEG渗调4小时,发芽率、生长势和活力指数分别由对照的62.5、3.05和1.95提高到90.6、7.88和7.15。实验表明,PEG可明显提高苦弧种子SOD和CAT的活性,并能抑制LOX的活性。经25%PEG处理后SOD和CAT的活性分别由对照的62.0、7.2提高到111.8和11.4,LOX活性由1.27降低到0. 相似文献
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小麦开花后根系膜脂过氧化作用及其对粒重的影响 总被引:1,自引:0,他引:1
研究了3个品种小麦开花后根系膜脂过氧化作用,以及对旗叶衰老、籽粒增重的影响,结果表明,小麦开花后,根系MDA含量和POX酶活性增加,SOD酶活性下降,膜脂过氧化作用加强。根系迅速衰老,引起旗叶叶绿素含量下降,旗叶早衰,籽粒增重缓慢千粒重低,蒲麦8441开花后植株早衰明显,千粒重低;阜阳861、扬麦158开花后植株衰老缓慢千粒重高。 相似文献
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