全文获取类型
收费全文 | 291篇 |
免费 | 14篇 |
国内免费 | 35篇 |
专业分类
林业 | 14篇 |
农学 | 51篇 |
基础科学 | 1篇 |
16篇 | |
综合类 | 170篇 |
农作物 | 32篇 |
水产渔业 | 1篇 |
畜牧兽医 | 6篇 |
园艺 | 32篇 |
植物保护 | 17篇 |
出版年
2023年 | 2篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2017年 | 11篇 |
2016年 | 5篇 |
2015年 | 11篇 |
2014年 | 17篇 |
2013年 | 15篇 |
2012年 | 24篇 |
2011年 | 26篇 |
2010年 | 19篇 |
2009年 | 20篇 |
2008年 | 30篇 |
2007年 | 29篇 |
2006年 | 28篇 |
2005年 | 23篇 |
2004年 | 17篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1989年 | 1篇 |
排序方式: 共有340条查询结果,搜索用时 15 毫秒
1.
苜蓿褐斑病抗性与几种同工酶的关系 总被引:6,自引:1,他引:5
以苜蓿5个品种在褐斑病抗性上存在差异的抗、感单株为材料,研究了接种病菌后苜蓿褐斑病抗性与多酚氧化酶(PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)及其酶活性的关系。研究结果表明,褐斑病侵染后,苜蓿抗病株系和感病株系叶片中PPO、POD和SOD同工酶谱带多少和强弱存在明显的差异,即大部分抗病株系PPO、POD和SOD酶活性高于感病株系,并有新的同工酶带出现。PPO、POD和SOD在苜蓿抗褐斑病上可能起到重要作用,其同工酶谱带和酶活性的差异有可能做为鉴定苜蓿抗褐斑病的一种同工酶标记。 相似文献
2.
3.
4.
为了缓解水蜜桃果实采后冷藏保鲜对果实品质的负面影响,达到更好的保鲜效果,以江苏省张家港市的凤凰水蜜桃(Prunus persica)为实验材料,研究了不同剂量(0.5kJ/m2、1kJ/m2、1.5kJ/m2)UV-C对低温(3℃?℃)条件下水蜜桃果实冷害和品质的影响。实验结果表明:三种剂量的UV-C处理对水蜜桃均能有效减少果实的褐变,保存果实的可溶性固形物(TSS),并明显延缓了丙二醛(MDA)、多酚氧化酶(PPO酶)的上升趋势;处理之间没有明显的效果差异,但1.5 kJ/m2处理的硬度值在5-20d之间均显著大于CK组(P<0.05),表现出更好的抗软化能力;另外,UV-C处理易使果实相对电导率加速升高,该趋势随照射剂量的增大而增强。 相似文献
5.
Clayton T Larue Joel E Ream Xuefeng Zhou Farhard Moshiri Arlene Howe Michael Goley Oscar C Sparks Steven T Voss Erin Hall Christine Ellis Janice Weihe Qungang Qi Daniela Ribeiro Xiaoping Wei Shirley Guo Artem G Evdokimov Marguerite J Varagona James K Roberts 《Pest management science》2020,76(3):1031-1038
6.
Effects of fruit bagging on coloring and related physiology,and qualities of red Chinese sand pears during fruit maturation 总被引:1,自引:0,他引:1
Chunhui Huang Bo Yu Yuanwen Teng Jun Su Qun Shu Zaiquan Cheng Liqiong Zeng 《Scientia Horticulturae》2009
Red Chinese sand pears (Pyrus pyrifolia Nakai) are particular to China. In order to determine the effects of fruit bagging treatments (including bag types, bag removal patterns and dates) on fruit qualities and to understand the mechanism of coloring of red Chinese sand pears, two experiments were carried out. In the first experiment, fruit of ‘Meirensu’ were firstly covered by light-impermeable paper bags with different levels of light permeable liners during their early development stage, then, the whole bag were/not removed or only the outer layer of bags were removed 3, 2 or 1 weeks before harvest. Thus, the fruit were/not totally re-exposed or were under different levels of sunlight transmission (80.31% or 34.71%). Non-bagged fruit were used as the control. Bagging treatments significantly affected the concentration of anthocyanin and the visual qualities of pear fruit. Compared to control, fruit re-exposed totally for 2 or 3 weeks accumulated the largest amount of anthocyanin and fruit receiving 80.31% and 34.71% of sunlight for 1–3 weeks could synthesize a little anthocyanin, indicating that high light intensity is imperative for coloring in red Chinese sand pears. Bagging treatments did not affect contents of total soluble sugars, but decreased organic acids contents in fruit. In the second experiment, fruit of ‘Meirensu’ and ‘Yunhongli No. 1’ were covered with only one type of light-impermeable bag during the early development stage and totally re-exposed after the bag removal 15 days before harvest. Fruit were then collected at different intervals to trace the time-course of coloring, and related physiology and inner qualities. With increasing time after the bag removal, the concentration of chlorophyll, carotenoid, flavonoid and total phenols changed little, but the concentration of anthocyanin accumulated extremely fast within 10 days after the bag removal in both cultivars and thereafter kept constant. ‘Yunhongli No. 1’ had higher anthocyanin contents and lower hue angle than ‘Meirensu’, indicating a higher potential of anthocyanin synthesis. After the bag removal, the sucrose contents and PAL activities increased gradually and correlation analysis revealed that they were highly correlated with anthocyanin accumulation in two cultivars. This study suggests that anthocyanin biosynthesis in red Chinese sand pears is a highly light dependent process and modified by genotypes. Based on the current results, in order to obtain red Chinese sand pear fruit with attractive appearance and good inner qualities, fruit must be covered with light-impermeable bags at the early stage of fruit development and the bag should be removed totally at least 10 days before harvest. 相似文献
7.
为探讨面粉白度与色泽相关因子的遗传特性及相互关系,选择11个在面粉白度等性状方面具有不同特点的小麦品种作亲本,按5×6不完全双列杂交设计组配杂交组合,对30个F1杂种和11个亲本的面粉白度与色泽相关因子进行了分析。结果表明,面粉白度、黄色素含量、多酚氧化酶活性在亲本表型值与其一般配合力效应值间存在显著的正相关。在亲本表型值间和一般配合力效应值间,面粉白度与黄色素含量均呈显著负相关,与多酚氧化酶活性间无显著相关性。面粉白度、黄色素含量和多酚氧化酶活性的广义遗传力和狭义遗传力都较高,主要受基因加性效应影响,适合在早代进行选择。德麦3号、济麦22号和济麦20号都能较好地降低后代的黄色素含量和多酚氧化酶活性,是小麦白度育种中较适宜选择的亲本。在优质中强筋小麦育种中,不应过分追求面粉的白度和亮度,但可通过对低多酚氧化酶活性和低黄色素含量的选择,培育面粉色泽优良的小麦新品种。 相似文献
8.
9.
鲜切莲藕中褐变因素分析的研究进展 总被引:1,自引:0,他引:1
鲜切莲藕的褐变问题很大程度上限制了产品的生产和销售,分析其褐变因素对解决贮运保鲜问题具有重要意义。本文针对文献资料介绍的导致鲜切莲藕褐变的各因素(酚类物质、多酚氧化酶及环境条件)进行总结和分析,阐明各因素在保鲜贮藏中的变化规律,以期为莲藕加工提供理论借鉴和技术指导。 相似文献
10.
南果梨多酚氧化酶的研究 总被引:20,自引:0,他引:20
南果梨果肉中多酚氧化酶(PPO)氧化邻苯二酚的活性明显高于氧化愈创木酚和间苯二酚的活性。以邻苯二酚为底物时,其多酚氧化酶的最适pH值为6.0。以儿茶酚为底物的多酚氧化酶氧化产物在420nm处有吸收高峰。聚乙烯吡咯烷酮(PVP)加入量与南果梨果肉酚类含量比为40:3时,南果梨果肉多酚氧化酶呈最大活性。30℃下多酚氧化酶的活性大于40℃、20℃、10℃、0℃下以儿茶酚为底物的南果梨果肉的多酚氧化酶活性。0.1MVc和001MEDTA对南果梨果肉的多酚氧化酶活性有明显抑制作用。根据凝胶电泳分析,南果梨果肉多酚氧化酶同功酶有8条谱带。 相似文献