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Kaoru Hanada Fumiyoshi Fukumoto Manabu Kusunoki Mitsuro Kameya-Iwaki Yuko Tanaka Toru Iwanami 《Journal of General Plant Pathology》2006,72(6):383-386
An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two
large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity
with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV.
The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656. 相似文献
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根据南芥菜花叶病毒(Arabis mosaic Nepovirus)CP基因序列,设计并合成了特异性RT-PCR检测引物,对南芥菜花叶病毒和非南芥菜花叶病毒的感病植物组织进行了PCR扩增反应。结果,南芥菜花叶病毒的感病植物组织的PCR产物均出现364bp的特异性扩增条带,而非南芥菜花叶病毒均未出现扩增条带,证明这对引物具有南芥菜花叶病毒鉴定特异性。将提取的南芥菜花叶病毒总RNA做梯度稀释,测定该检测体系的敏感度,结果表明,此体系最低可检出南芥菜花叶病毒提取的RNA模板浓度为150pg/μL。 相似文献
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Artemis Rumbou Susanne von Bargen Carmen Büttner 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(3):527-532
The wide natural incidence of Cherry leaf roll virus (CLRV) in deciduous forest trees and nurseries in northern Europe is believed to have occurred, apart from occasional mechanical
spread and transmission through grafting, mainly by seed transmission. The mode of the vertical transmission and its role
in the epidemiology of the virus has not been investigated, basically due to the inconvenient host-pathogen combinations studied
to date. With the aim of obtaining an appropriate system for identification of viral genes and products participating in infection
processes and seed transmission of CLRV, we performed infection and seed transmissibility tests with CLRV in Arabidopsis thaliana plants. Two phylogenetically and serologically different CLRV isolates were tested. Both of them were found able to infect
A. thaliana plants, exhibited clear symptoms of the infection and spread systemically in the plants. Infection of the seeds and of a
remarkable number of seedlings generated from infected seeds was possible for two consecutive generations. These results,
for first time, report seed transmission of CLRV in the model plant A. thaliana and allow the assumption to be made of embryo invasion during seed transmission. Furthermore, first indications are given
that genetically diverse CLRV isolates exhibit different abilities for vertical transmission in A. thaliana. The CLRV-A. thaliana model system is suitable for investigating viral invasion of developing plant organs and meristematic tissue, a prerequisite
for successful virus dissemination via vertical transmission through seed. 相似文献
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线虫传多面体病毒属病毒在全世界温带地区均有分布,具有广泛的寄主范围,其中许多种病毒能够在农业生产中引起严重危害。从传播途径、病毒种类、分类依据、基因组结构与功能等方面对线虫传多面体病毒属进行概述。 相似文献
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