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排序方式: 共有815条查询结果,搜索用时 31 毫秒
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Single nucleotide polymorphisms (SNPs) of cytokine genes have been found to be involved in the clinical outcome of Tuberculosis. The present study was aimed to identify the high risk genotypes in Tuberculosis patients and their household contacts. A total of 490 subjects were studied which includes 150 active pulmonary tuberculosis patients (APTB), 190 household contacts (HHC) and 150 healthy controls (HC). The SNPs of TNF-α (-308A/G), IL-10(-1082G/A) and IL-6(-174G/C) were performed by ARMs PCR. The IL-10 GA genotype showed significant association in APTB and HHC and was 2.3 times higher risk in APTB and 3.7 times in HHC compared to HCs. The A allele was found to be significantly associated with the risk of disease. The CC genotype of IL-6 was found to be significantly associated in APTB and an insignificant positive association in HHCs. The multifactor dimensionality reduction (MDR) analysis indicated that the genotypes of IL-6 were showing high risk with GA genotype of IL-10. In conclusion the gene interaction may be useful for identification of genotypes as biomarkers to distinguish high risk individuals. 相似文献
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Inheritance and QTL Mapping for α-Tocopherol in Soybean 总被引:1,自引:1,他引:0
LIANG HuiZhen YU YongLiang XU LanJie YANG HongQi DONG Wei TAN ZhengWei LI Lei PEI XinYong LIU XinMei 《中国农业科学》2019,52(1):11-20
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In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α. 相似文献
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运用矩阵分析方法,讨论了非奇异H-矩阵的判定问题,得到两个非奇异H-矩阵新的判定准则,并以数值例子说明判定方法的有效性. 相似文献
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本研究旨在探讨牦牛不同部位皮肤内血管和神经的分布情况,及检测低氧诱导因子-1α(HIF-1α)在不同部位皮肤上的定位及相对表达量,探究牦牛皮肤对高原低氧环境的适应机制。采用HE、Masson’s三色和Verhoeff VG染色法,对成年牦牛皮肤内血管和神经结构进行观察与分析;采用免疫组织化学、实时荧光定量PCR和蛋白免疫印迹法,对HIF-1α的mRNA和蛋白在成年牦牛皮肤组织中表达与分布进行研究。结果表明,颈部血管与神经密度最高,前臂部和小腿部次之,跖部最低,部位间差异显著(P<0.05)。HIF-1α主要表达在表皮层、毛囊的上皮根鞘、皮脂腺、汗腺、血管、神经;颈部、前臂部和小腿部强阳性表达,跖部阳性表达。HIF-1α mRNA的相对表达量跖部明显低于其他部位(P<0.05),其他三个部位两两比较差异不显著(P>0.05)。HIF-1α蛋白相对表达量颈部最高,跖部最低,差异显著(P<0.05)。研究结果提示,成年牦牛不同部位皮肤内不同血管和神经形态结构相似,密度从颈部到前肢再到后肢差异显著。HIF-1α的差异性表达进一步说明皮肤在牦牛适应低氧环境中发挥作用。 相似文献
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Monika Jamioł Jacek Wawrzykowski Marta Kankofer 《Reproduction in domestic animals》2021,56(7):1040-1049
One of the most important processes determining the proper course of gestation and its physiological termination in cows is the adhesion of epithelial cells allowing for direct contact of maternal and foetal parts of the placenta. Throughout pregnancy, placental cells are under strict hormonal control, which among others regulates the concentration and activity of specific proteins participating in the extracellular matrix remodelling of foetal membranes. The aim of the study was to evaluate the influence of progesterone and prostaglandin F2α on the adhesion of epithelial cells at early-mid pregnancy in cows. Additionally, the impact of selected hormones on anti-adhesive properties of decorin was evaluated. Caruncular epithelial cells were isolated from healthy cows during pregnancy, immediately after slaughter. Primary cell cultures derived from the 2nd and 4th month of gestation were used in the experiments. The viability of cells was assessed by MTT assay. The adhesion of cells to fibronectin was measured spectrophotometrically. The activity of metalloproteinases was confirmed by the metalloproteinase assay. Progesterone (10–5 and 10–7 mol/L) and prostaglandin F2α (10–4, 10–5 and 10–7 mol/L) increased the viability of bovine caruncular epithelial cells in the 2nd month of pregnancy. The treatment with prostaglandin F2α significantly reduced the number of adherent cells from the 2nd month of gestation at the doses of 10–4 and 10–5 mol/L. Both progesterone and prostaglandin F2α were shown to have an effect of decorin resulting in both a decrease in metalloproteinase activity and an increase in adhesion of cells to fibronectin. 相似文献
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ZHANG Qiong WAN Chang-wu YU Yan-ni XIA-Bing LIU Jiang-jin ZHANG Qiao-jun LI Zhu WANG Cheng-fei DAI Jia-lin WANG Jie 《园艺学报》2021,36(12):2133-2138
AIM To explore the possible mechanism of cathepsin C (CTSC) and tumor necrosis factor-α (TNF-α) in coronary heart disease (CHD) by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees (P <0.05). The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group (P <0.05). Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group (P <0.05). The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD (r2 =0.743, P <0.05). CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD. 相似文献