猪子宫内膜基质细胞和腺上皮细胞的分离培养

为了获得高纯度的猪子宫内膜基质细胞和腺上皮细胞,对猪子宫内膜进行胶原酶消化,分离基质细胞和腺上皮细胞进行体外培养,研究其生长特性.结果表明:猪子宫内膜组织在37℃、通过1 g/L胶原酶Ⅰ消化90 min可以获得游离的腺体,对腺体进行培养时上皮细胞比例可达70％以上.培养过程中采用差速消化和差速贴壁可以成功分离腺上皮细胞和基质细胞.基质细胞最适贴壁时间为25 min,腺上皮为3h.纯化的基质细胞生长迅速,可传5代以上,上皮细胞生长缓慢,并且成片脱落.     

台湾过山香组培快繁技术研究

以当年生台湾过山香带腋芽半木质化茎段作为外植体,开展组织培养试验研究,建立组培快繁体系。结果表明:最佳外植体诱导培养基为1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,诱导率可达89.7%;最佳增殖培养基为DCR+6-BA 0.8 mg/L+NAA 0.1 mg/L,增殖率达5.24倍,继代周期为35 d,不定芽生长状况好;最佳生根培养基为1/2MS+IBA 0.5 mg/L+ABT 1生根粉0.4 mg/L,生根率达95.26%;以泥炭土:黄心土:珍珠岩:钙镁磷肥(3.0:2.0:1.0:0.2),移栽成活率达93.7%,且7 d缓苗,茎秆木质化迅速,茎《浓绿,长势好。     

迷迭香扦插育苗试验初报

迷迭香(Rosmarinus officinalis L.)是唇形花科迷迭香属植物,是一种多年生常绿亚灌木,为观赏及芳香油植物,该植物已成为全球普遍重视的资源植物之一。迷迭香种子发育不良,种子萌发力极低,故通常采用无性繁殖法育苗,在生产中为了降低成本,节约资金,现阶段主要采用扦插法繁殖进行苗木生产。近年来,国内外对于迷迭香精油及抗氧化剂的研究报道及论文很多,但对迷迭香育苗技术报道却较少。开展该课题研究,对迷迭香的育苗技术进行研究,通过试验记载、观察、田间管理,为迷迭香种植提高育苗成效,降低成本,增加农民收入,为中国天然抗氧化剂的生产保障优质充足原料供应,具有重要意义。试验研究在玉溪对迷迭香进行扦插试验。迷迭香分别扦插在基质为菜园土的苗床上、用纸杯装菜园土扦插、在泥炭土:珍珠岩为2:1的基质上扦插,试验结果表明,试验结果表明,春季扦插成活率高,成活时间短,扦插苗生长健壮,在菜园土上扦插成本低,用纸杯装土扦插,成活率95%￣99%,且苗生长健壮。在泥炭土:珍珠岩为2:1的基质上扦插结果,嫩梢作插穗在插后8d开始生根,25d全部生根,生根率99.5%。以上各种扦插方法培养的扦插苗移栽成活率均在95%以上,没有明显区别,可见3种方法扦插育苗都具有成活率高的特点,但采取什么方法,以成本和已有的生产材料及技术而定。     

不同基质对铁皮石斛组培苗成活和生长的影响

试验设3种基质处理,即刨花∶细锯末=1∶6(处理1)、桤木树皮∶细锯末=5∶1(处理2)、杉木树皮∶细锯末=5∶1(处理3),比较铁皮石斛的成活率、茎粗、株高、根长、根数、叶片数等,筛选适合铁皮石斛生长的最佳基质。该试验条件下,移栽30 d后铁皮石斛组培苗成活率在处理1中为91.3%,在处理2中为97.5%,在处理3中为95.5%;移栽60 d后,其株高、根长在处理1中为6.40、3.26 cm,在处理2中为7.40、5.25 cm,在处理3中为6.45、5.10 cm;其根数在处理1、2、3分别为5.0、6.5、5.6条,茎粗则分别为0.255、0.582、0.542 cm,对应的叶片数分别为9.0、14.5、13.0片。研究初步显示,青木树皮∶细锯末=5∶1是用于铁皮石斛试管苗移栽的最佳基质。     

S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce Tenascin C synthesis under DMSO exposure in vitro

In breast cancer research S100A4‐positive tumour‐associated stromal cells are assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4/TNC positive stromal canine mammary tumour (CMT) cells. Cells grown as scaffold‐free spheroids were investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well‐known side effects on cells including TNC expression. DMSO did not affect proliferation, but TNC was significantly reduced under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was only observed after 14 days. Without DMSO, cells stably expressed TNC and S100A4 which makes them suitable to be used in experimental approaches requiring S100A4/TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not be used as solvent for experiments concerning TNC/S100A4 expression.     

有机生态栽培基质配比对辣椒产量及品质的影响

以菇渣、锯木屑和珍珠岩3种基质为材料,探讨其不同配比对辣椒产量及品质的影响。结果表明:当菇渣、锯木屑和珍珠岩的体积配比分别为30%,50%和20%,能显著提高辣椒产量,增加果实维生素C和粗蛋白含量,降低硝酸盐含量。因而,有机生态型无土栽培技术在辣椒增产增收和改良品质上具有重要意义。     

An investigation into the epidemiology of Strongylidae infections in the horse in the Netherlands

Summary

In a four‐year survey, the course of pasture infestation with infective larvae of Strongylidae and the pattern of development of the Strongylidae infection in the host were investigated. In this way it was hoped to obtain epidemiological data which might serve as a basis for the control of strongylidosis in the horse.     

基质与肥料对紫杉扦插生根率及生长量的影响

通过不同基质对紫杉插穗生根率影响、不同肥料种类对紫杉生长量和生长状况的影响的试验研究与分析,结果表明:混合土层分层基质扦插生根率高,施用腐熟香油渣混合液,所含N、P、K等各种紫杉生根所需要营养元素较均衡,苗木年生长量最大。