海东地区荒山荒坡三种治理措施的效果对比研究

对治理荒山荒坡常用的封育、补播、重新建植三种方法进行了对比、调查和分析。结果表明,采用补播措施治理荒山荒坡是较为有效的途径,也是合理开发利用荒山荒坡、尽快恢复植被、控制水土流失和改善生态环境经济实用而又可行的方法。     

不同播期和不同肥力条件下向日葵干物质形成和物质分配对杂交种产量的影响

不同的播期明显影响向日葵籽实产量及病害发生的程度。通过调整播期，可防止或减轻叶部斑病和菌核病的发生，在向日葵生产上起着很大作用。向日葵不同品种干物质积累规律符合Logistic生长曲线，可用方程y=k/(1 ae^-bx）模拟。向日葵植株的器官平衡依次为：除空壳外，茎秆（28.5%)、籽实（24.5%)、叶片（22.5%)、葵盘（17.3%)、叶柄（4.9%)。土壤肥力对向日葵的器官平衡有较大影响。经济系数不高是向日葵的一个特点。栽培条件不同，经济系数差异较大。在一般情况下，春播条件下经济系数较高，夏播条件下经济系数较低。     

奎屯地区气象要素对玉米播种及苗期的影响

奎屯地区农七师位于天山北麓,准噶尔盆地的西南缘,总面积5986km2,其中作物种植面积120万亩,随着农七师产业结构的调整,2003年实施了“万头澳牛引进工程”,养牛业的发展使农七师畜牧业对玉米的需求量增加,尤其是澳牛养殖业对青贮玉米需求量更大,因此,研究分析气象要素对玉米播种及苗期的影响是重要工作之一。     

近交系大鼠DNA指纹图与微卫星DNA比较分析

为了建立一种对近交系大鼠遗传物质进行精确可靠、快速简便的监测方法,利用DNA指纹技术对国内6个品系8个近交系大鼠群体进行了DNA多态性的分析,并与PCR扩增微卫星DNA技术进行了比较.其结果显示(1)不同品系之间DNA指纹图差异较大,同一群体不同个体间DNA指纹图带的相似系数和共有带率除SHR(哈)和WKY(哈)小于0.7外,其他均大于0.9.不同地区同一SHR间和WKY间DNA指纹图也存在差异,相同DNA不同次制作的DNA指纹图谱基本一致.(2)不同品系个体间微卫星DNA具有显著多态性;同一群体不同个体之间除SHR(哈)的SMST位点和WKY(哈)的AGT位点出现一定的差异外,其他均没有差异.DNA指纹图能更精确可靠地反映出动物个体间的遗传背景,而微卫星DNA遗传监测方法比较简便快捷.     

水稻机械化喷直播技术的应用现状及对策

分析了水稻机械化喷直播技术的特点和技术要求，介绍了水稻机械化喷直播技术的应用现状及其技术推广的制约因素．提出了推广水稻机械化喷直播技术的对策．     

A grower-friendly method to transfer predacious mites to commercial orchards

The establishment of predacious mites in commercial orchards may be accelerated by the transfer of pruned wood in winter and summer from donor orchards to release orchards. Following winter pruning, 3-year-old and older wood is collected and transported as soon as possible in bundles to a release orchard for distribution. If the release orchard is composed of dwarf trees, then one or two bundles of 5 kg each are placed vertically at the base of the trunk of every tree in the block (0.5 to 1 ha); if the trees are of standard size, then four or five bundles used. Following summer pruning, annual shoots and suckers are distributed immediately in a release orchard composed of dwarf trees by placing 12–15 branches on the foliage of fruit-bearing branches; if the release orchard is composed of standard trees, then 50 branches are used. The pruned wood should have 20–25 leaves and not less than one predator per leaf. The release orchard should have a light infestation (two or three mites per leaf) of pest tetranychids. These phytophagous mites would serve as food and help establish the predators. The release orchard grower should develop a pest management program based on the same groups of pesticides used in the donor orchard. http://www.phytoparasitica.org posting Aug. 31, 2005.     

猪原始生殖嵴细胞(PGCs)建系因素的研究

从五指山猪(WSZP)近交系第8～13代培育群中，先后选用21头5～10月龄青年母猪，分别于授精后25～30d采集胎儿106个，进行原始生殖嵴(PGCs)细胞分离、培养等建系技术研究。以DMEM F10(1:1)为基础培养液，按添加或不添加生长因子，将培养液分为A、B、C3种，并以STO细胞作饲养层，在38℃、5.0％CO2和湿润的气相中进行培养建系。结果获得胚胎生殖嵴细胞(EG)细胞系6个细胞株，其中1个EG细胞株传至11代、2个传至5代、1个传至4代、2个传至3代冻存。并进行了AKP染色、体外分化、冷冻-解冻复苏和嵌合体制作等鉴定研究。研究发现：不同胚龄对EG细胞建系具有一定影响，不同培养液对EG细胞建系效果不同，STO细胞饲养层的质量是建株、传代、冷冻-解冻复苏的关键因素之一。EG细胞系的初步建立，为今后筛选进入种系的EG细胞系、实施体外基因操作提供了可能。     

Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method

ABSTRACT

1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host–pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required.

2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces.

3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment.

4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacter spp. assessment.

5. All liver and 28/30 caeca samples tested positive for Campylobacter spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts.

6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.     

铺地竹叶片对结球生菜种子发芽及幼苗生长的影响

采用基质盆栽试验,研究了铺地竹(Sasa argenteastriatus E.G.Camus)叶片对结球生菜(Lactucasativa L.var.capitata L.)种子萌发和幼苗生长的影响,结果表明,铺地竹叶片粉末对结球生菜种子的萌发和幼根生长具有一定的抑制作用,随着浓度的增加抑制作用增强,在50～200 g/m2的范围内,幼根抑制率为12.15%～28.91%。在50～100 g/m2的用量下,铺地竹叶片对结球生菜苗长表现为促进生长作用,而在200 g/m2时,则表现为抑制生长作用。施用铺地竹叶片后3 d、6 d、9 d和12 d,结球生菜幼苗的叶绿素含量特别是叶绿素a的含量显著减少,随着基质中叶片粉末浓度的增加,结球生菜叶绿素含量降低,叶绿素a含量高低依次为对照、低浓度处理、中浓度处理、高浓度处理。随着处理后时间的延长,竹叶对结球生菜叶片叶绿素a含量的影响减弱。这个结果进一步验证了自然条件下铺地竹叶片对植物生长的化感效应,说明其具有开发植物源除草剂的潜力,为研究铺地竹叶片中的化感物质提供了佐证。