食用菌复合保水颗粒菌种生产工艺及其应用研究

运用微生物发酵技术，配合新型合成材料，研制出食用菌复合保水颗粒菌种。这类菌种可保持活力60d。这一工艺可用于糙皮侧耳、金针菇、灵芝、白灵菇、杏鲍菇、鸡腿蘑和鲍鱼菇等多种食用菌苗种的制作。栽培试验证明，此类菌种的菌丝生长速度、现蕾时间、生物学效率与常用的棉籽壳菌种相似。     

Surgical management of bilateral,upper and lower eyelid entropion in 27 Shar Pei dogs,using the Stades forced granulation procedure of the upper eyelid only

ABSTRACT

Aims: To evaluate the outcome of surgical management of entropion of the upper and lower eyelids in Shar Pei dogs which had previously undergone temporary palpebral tacking, using the Stades forced granulation procedure of the upper eyelid only.

Methods: Medical records were retrospectively searched to identify Shar Pei dogs referred for bilateral entropion of both the lower and upper eyelids, and that were treated with the forced granulation procedure of the upper eyelid only. Dogs were included if they had previously undergone unsuccessful temporary palpebral tacking and had at least three follow-up examinations in the 30 days following surgery.

Results: Twenty-seven Shar Pei dogs with a median age of 7.9 (min 4, max 24) months were included in the study. Before surgery, all dogs showed signs of severe ocular discomfort with bilateral keratitis and visual deficit due to blepharospasm, enophthalmos and protrusion of the third eyelid. In 13/54 eyes, keratitis was associated with a corneal ulcer. When re-examined 4 weeks after surgery, correction of the upper eyelid entropion and associated trichiasis resolved ocular signs in 50/54 eyes. Mild bilateral lower entropion remained in two dogs postoperatively, which underwent revision surgery with the Hotz-Celsus technique. There were no cases of long-term recurrence of entropion or ocular irritation in the 38 eyes (19 dogs) which were re-examined 1 year after surgery.

Conclusion and clinical relevance: The forced granulation procedure performed on the upper eyelid only was effective for correction of entropion in the Shar Pei dogs included in this study. In our experience, it is preferable to operate on the upper eyelid alone, rather than attempting to correct upper and lower entropion during the same surgical operation. The dog can then be reassessed a few weeks later to determine whether the lower entropion is anatomical or secondary to the severe blepharospasm resulting from the painful ocular irritation.     

转盘锤磨机的研发及应用

天然标准橡胶加工的锤磨搓揉、除杂是天然橡胶生产中的关键环节。锤磨机广泛应用于大型天然橡胶初加工生产线，其锤磨搓揉、造粒除杂效果是转盘锤磨机的重要指标，直接影响绉片等后序工艺的加工质量。研发的转盘锤磨机提高了胶块搓揉、造粒、除杂效果及改善了生产工艺，同时也提高了产品质量和产量。     

有机-无机复混肥圆盘造粒试验研究

对有机-无机复混肥圆盘造粒进行试验研究，分析了圆盘转速、圆盘倾角、喷头喷孔直径以及水分含量对颗粒成粒率和颗粒抗压强度的影响，确定了有机一无机复混肥圆盘造粒时较适宜的圆盘倾角、圆盘转速和水分含量。     

UASB反应器中厌氧污泥颗粒化的微生物学机理

通过对容积为5升的UASB反应器启动试验,研究了反应器内以葡萄糖为基质污泥颗粒化过程中不同类群细菌的消长规律。依据对颗粒化过程中细菌行为的观察,初步提出了厌氧颗粒污泥形成的机理,讨论了细菌种类对颗粒污泥性能的影响。     

湖北三峡库区晚熟脐橙不同海拔果实品质变化及枯水防控技术研究

以湖北三峡库区秭归地区不同海拔伦晚脐橙（Citrus sinensis Osbeck ‘Lane late’ Navel）果实为试验材料,探讨了湖北三峡库区晚熟脐橙果实枯水防控栽培技术,进行了晚熟脐橙种植区域布局研究,结果表明,在湖北三峡库区,江南晚熟脐橙适宜区域化种植在海拔360 m以下,江北在海拔350 m以下。在适宜范围外,随海拔升高,晚熟脐橙果实品质下降,可食率、果汁率降低,酸下降快,化渣性变差,果实枯水加重。其次,探讨了晚熟脐橙果实枯水防控生产技术,冬季套袋试验效果好,果实枯水比率控制在5%以下,显著低于对照果实（枯水率50%）,可有效减轻和防止晚熟脐橙果实枯水,而且套袋果实外观好,品质优。覆膜技术效果其次,果实枯水比率可以控制在20%以下,而且该技术简便、劳动量小且成本较低,可以在生产中应用。     

琯溪蜜柚砂囊粒化的无损检测

摘 要：柑橘砂囊粒化是柑果成熟期与采后贮藏期的常见生理性病害,严重影响果实品质。本文采用1.5 T 高场磁共振成像仪对采后贮藏30 d的琯溪蜜柚[Citrus grandis (L.) Osbeck]果实进行磁共振扫描检查,并以同层断面解剖图为对照,评估磁共振成像（MRI）应用于柑橘砂囊粒化症无损检测的有效性。结果表明, MRI检测中,T2WI能够显著区分果肉组织与海绵层组织的信号差异,其信号强度可代表果实组织相对含水量;T2WI信号在砂囊粒化部位强度显著降低,病变组织的失水特征清晰,与解剖结果一致。T2WI对砂囊粒化的确诊率100%,显著优于T1WI成像的识别率41.7%（χ2 = 5.76,P = 0.016）;T2WI和T1WI均能显示种籽照影,但T2WI对果肉组织影像的分辨率优于T1WI,因此MRI的T2WI分析可作为一种无损检测方法,对柚商品果实上市前砂囊粒化症发生程度以及种籽有无进行评估。     

Alteration of the haemocyte parameters,expression of immune‐related and neurohormone bursicon genes of giant river prawn Macrobrachium rosenbergii (de Man) in the response to salinity stress and ammonia stress

We examined the effects of salinity stress and ammonia stress on alteration of the haemocyte count, phenoloxidase activity, expressions of immune‐related genes including prophenoloxidase (proPO), crustin, heat shock protein 70 (HSP70), and expressions of stress‐responsive neurohormone (Bur‐α and Bur‐β) in the thoracic and abdominal ganglia of giant river prawn Macrobrachium. These parameters of prawn that subjected to salinity stress (transferred from 0‰ to 5‰ and 10‰), and subjected to ammonia‐nitrogen (ammonia‐N) stress (transferred from 0 to 0.262 mg/L and 0.786 mg/L) were examined after 0, 3, 6, 24, 72 and 168 hr respectively. During the initial period of 3, 6 and 24 hr, granulocyte haemocyte (granular and semi‐granular hemocyte) count and PO activity significantly decreased, while expressions of Bur‐α and Bur‐β significantly increased. After 24 hr, granulocyte haemocyte count and PO activity significantly increased, whereas expressions of Bur‐α and Bur‐β significantly decreased. The expressions of proPO, crustin and HSP70 were significantly downregulated in the prawn that subjected to salinity stress and ammonia‐N stress at all time periods of 3–168 hr. In conclusion, changes in the granulocyte haemocyte count of M. rosenbergii following salinity stress and ammonia‐N stress are closely associated with the changes of Bur‐α and Bur‐β expressions.     

鲍蒸煮液复合调味品制作配方和工艺研究

为了提高鮑资源的利用率,开发新型香精,以鲍蒸煮液美拉德反应产物为基料,感官评定为指标,在单因素及L_9(3~4)正交试验基础上,确立了口感和海鲜风味俱佳的鲍复合调味品最佳配方为:盐添加量35%、味精添加量40%、玉米淀粉添加量9%、鲍风味基料添加量为6%、白砂糖添加量4%、麦芽糊精添加量3%、呈味核苷酸二钠(I+G)添加量1.5%、酵母抽提物添加量1.5%。研究了不同的造粒方式和干燥方法对颗粒产品吸湿性、溶解性、流动性、质构特性及感官评定的影响;相关理化、性能指标为:完全溶解于90~100℃热水中的时间需要72 s,且分散良好、溶液微浑浊、无沉淀,鲜味足;堆密度为(0.61±0.01)g/cm~3,硬度为(3 642±122.198)g,吸湿性为(9.35±0.01)%,临界湿度值为60%,休止角为33.0°,总体可接受度为(7.81±0.12)分。研究结果表明,旋转造粒和流化床干燥的产品质量最佳,吸湿性比较小,溶解性、流动性和质地性都比较好,感官评分最高。