The Value of Tissue Imprint Hybridization for Rapid Detection of Infectious Bursal Disease Virus from Field Outbreaks

The use of a peroxidase labelled PCR generated probe followed by enhanced chemiluminescence hybridization assay detected infectious bursal disease virus directly from bursal imprints on a nylon membrane. Tissue imprint hybridization proved to be a simple, rapid and safe means of detecting IBD virus for screening large numbers of field samples. The PCR generated probe was highly specific for IBD virus and did not hybridize with cellular nucleic acids in control imprints. Tissue imprint hybridization was found to be a more sensitive method than conventional antigen detection assays.     

5种弥散神经内分泌细胞在鸭腔上囊中的表达特征

应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长押素在鸭腔上囊中的表达特征.结果显示鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应.结果说明,鸭腔上囊中存在β-内啡肤、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育.     

法氏囊在鸡淋巴细胞性白血病发生发展中的作用

用鸡淋巴细胞性白血病病毒ＲＡＶ－１株接种３５只１日龄伊莎鸡雏，于接毒后不同批次扑杀，采取法氏囊做组织学、免疫细胞化学、透射电镜观察。结果：接毒后１个月，法氏囊滤泡髓质淋巴细胞开始转化，接毒后２～５个月更明显，在法氏囊滤泡髓质区形成成淋巴细胞克隆增殖灶。接毒后６个月，法氏囊萎缩。生物素－亲和素（ＢＡ）法染色表明法氏囊一直存有病毒和群特异性抗原，以接毒后３～４个月含量最高。电镜观察，在接毒后１～４个月的实验鸡法氏囊滤泡髓质淋巴细胞、巨噬细胞和网状细胞中观察到淋巴细胞性白血病（ＬＬ）病毒粒子。组织学、免疫细胞化学和电镜观察都表明法氏囊是该病的主要靶器官之一，法氏囊在鸡淋巴细胞性白血病的发生发展中起一定的作用     

囊素的研究进展

为更好地研究和利用囊素的免疫调理活性，从囊素的产生、存在部位、生物活性及其作用机理等方面论述了囊素的研究现状，指出了有关囊素的亟待解决的问题和应用前景。     

法氏囊活性肽对新城疫La Sota株弱毒疫苗的免疫增强效果

将7日龄黄羽肉鸡300只均分为对照、常规疫苗剂量配合法氏囊活性肽、常规疫苗剂量减半配合法氏囊活性肽、生理盐水和法氏囊活性肽5组,通过血凝抑制试验测定血清抗体水平和间接ELISA检测鼻腔冲洗液中IgA水平及计算法氏囊指数等试验方法,研究法氏囊活性肽和新城疫La Sota弱毒疫苗配合应用的免疫效果。结果显示,29日龄后,常规免疫剂量配合法氏囊活性肽组的法氏囊指数、血清抗体滴度和IgA水平显著高于对照组(P<0.05),而常规剂量减半配合法氏囊活性肽组与对照组在法氏囊指数、血清抗体滴度和IgA水平上差异不显著(P>0.05);说明法氏囊活性肽对疫苗有免疫增强作用,疫苗剂量减半配合法氏囊活性肽应用可达到常规疫苗免疫效果。     

POSITIVE CONTRAST MAGNETIC RESONANCE BURSOGRAPHY FOR ASSESSMENT OF THE NAVICULAR BURSA AND SURROUNDING SOFT TISSUES

Magnetic resonance (MR) imaging is often performed to determine the cause of palmar heel pain. We evaluated how distension of the navicular bursa affected the MR appearance of the navicular bursa and associated structures. An MR evaluation was performed on normal cadaver limbs and cadaver limbs from horses with lameness localized to the foot. The normal navicular bursae were injected with 2, 4, or 6 ml of solution. The bursae of the feet from lame horses were injected with 4 or 6 ml, and the MR study was repeated. All bursae were examined grossly to verify the presence or absence of adhesions. Clinical patients that had initial MRI abnormalities suggesting adhesions were also evaluated. Distension of the proximal recess of the normal navicular bursa, proximal to the collateral sesamoidean ligament was achieved with 2 ml. Separation of the collateral sesamoidian ligament from the deep digital flexor tendon (DDFT) was achieved with 4 ml. The separation of the navicular bone from the DDFT and distal sesamoidian impar ligament required 6 ml. Adhesions were more clearly defined in the bursa of the two pathologic cadaver limbs following distension. MR bursography used on clinical patients allowed the determination of the presence or absence of adhesions. In these horses, this determination could not have been definitively made without this technique. MR bursography is useful in horses where the presence of adhesions cannot be clearly defined by MRI.     

广西新黄鸡腔上囊和胸腺的发育及其T淋巴细胞的分布

10日龄鸡胚腔上囊开始形成囊腔和皱褶,并偶尔见到T淋巴样细胞;12日龄鸡胚出现淋巴滤泡;17日龄鸡胚滤泡髓质基本形成;3日龄雏鸡滤泡皮质出现。18日龄鸡胚开始形成DIA,随日龄的增长,DIA由腔上囊背侧近导管开口处逐渐扩展到导管背侧;7周龄前后到达与泄殖腔相连处。随着滤泡及DIA的形成,T淋巴样细胞分别出现于其中。滤泡中T淋巴样细胞的百分率,胚胎期大于孵出后。出生后T淋巴样细胞主要聚集于DIA内。9～11周龄腔上囊生长达到最大值,21.5～52周龄腔上囊消失。 14日龄鸡胚胸腺开始出现髓质,并可在其中检出ANAE细小颗粒型细胞,其百分率出生后大于胚胎期。但10～12日龄鸡胚胸腺中有大量的未成熟的胸腺细胞呈ANAE斑点型反应     

ULTRASONOGRAPHY OF THE EQUINE BICIPITAL TENDON REGION: A CASE HISTORY REPORT AND REVIEW OF ANATOMY

The equine shoulder region is difficult to investigate by conventional imaging modalities. Diagnostic ultrasound has proven valuable for investigation of soft tissue injuries of the human shoulder. In a horse with shoulder lameness, the authors employed diagnostic ultrasound to augment the use of conventional radiography. A defect in the subchondral bone layer of the medial humeral tuberosity, with adjacent bony fragments were clearly identified with ultrasound. Ultrasound was used for initial evaluation of the bicipital tendon and bursa. The technique was subsequently used to follow and evaluate the post-operative condition of the proximal cranial humerus, bicipital tendon and bursa in this patient. The bicipital regions of 2 immature and 3 adult normal horses were also examined with to illustrate the normal ultrasonographic anatomy of the cranial shoulder region.