Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.     

Long-term effects of TCV116 on left ventricular remodeling and heart function after myocardial infarction in rats

AIM: To investigate the effects of long-term TCV116 on left ventricular remodeling and heart function after myocardial infarction. METHODS: Myocardial infarction (MI) was caused by ligation of the left anterior descending coronary artery in rats. One week after the surgical performance, the surviving rats were randomly assigned to the following treatment protocols: (1) MI rats with no therapy; (2) MI rats treated with TCV116 2 mg/kg per day; (3) Sham-operated control; (4) Sham-operated rats, treated with TCV116 2 mg/kg per day. At 22 weeks, cardiac hemodynamic parameters such as MAP, LVSP, dp/dt_max and LVEDP, and histomorphometric parameters such as LVW/BW and LVCA/BW were measured, mRNA of cardiac genes such as βMHC, BNP, TGF-β₁, collagen I and III were quantified, and survival rates were calculated. RESULTS: Compared with sham-operated rats, MI rats without therapy showed significant increases in histomorphometric parameters as well as in mRAN expressions of cardiac genes (P<0.01); While their hemodynamic parameters were significantly impaired (P<0.01), and survival duration shortened (P<0.05). Compared with MI rats without therapy, MI rats treated with TCV116 showed significant attenuation of mRAN expression of cardiac genes (P<0.01); While their hemodynamic parameters were significantly improved (P<0.05 or P＜0.01), and survival duration extended (P<0.05). CONCLUSION: Treatment with long-term angiotensin II type 1 receptor antagonist may improve left ventricular remodeling and cardiac function after MI in rats.     

反义抑制ghrelin对VEGF及其受体Flt-1 mRNA表达的影响

[目的] 探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1 mRNA表达的影响.[方法] 试验分为4组: I组,空白对照组;II组,脂质体组(不加寡核苷酸);III组(SCON),20 μmol/L正义寡核苷酸组;IV组(ASCON),20 μmol/L反义寡核苷酸组.上述各组均在培养24、36、48 h后,采用实时荧光定量方法检测VEGF及其受体Flt-1 mRNA表达情况的变化.[结果] VEGF和Flt-1熔解曲线表明扩增效果好,培养24 h, VEGF mRNA I组相对表达量为0.076 9,II组为0.076 8,III组为0.076 9,3组之间无显著性差异(P＞0.05),IV组VEGF mRNA 相对表达量下降为0.024 2,且与I、II、III组有显著性差异(P＜0.05).培养36 h,前3组相对表达量分别为0.059,0.051,0.051 4,3组之间无显著性差异(P＞0.05),第IV组为0.024 2,与前3组比较有显著性差异(P＜0.05).培养48 h,前3组相对表达量分别为0.061,0.055 2,0.052 1,3组之间无显著性差异(P＜0.05),第IV组为0.011 5,与前3组比较有显著性差异(P＜0.05).培养24 h,Flt-1 mRNA I组相对表达量为0.078 6;II组为0.078 2,III组为0.078 1, 3组之间无显著性差异(P＞0.05),IV组Flt-1 mRNA 相对表达量下降为0.025 6,且与I、II、III组有显著性差异(P＜0.05).培养36 h,前3组相对表达量分别为0.078 1,0.078 4,0.078 2,3组之间无显著性差异(P＞0.05),第IV组为0.022 8,与前3组比较有显著性差异(P＜0.05).培养48 h,前3组相对表达量分别为0.069 1,0.078 0,0.069 3,3组之间无显著性差异(P＞0.05),第IV组为0.021 5,与前3组比较有显著性差异(P＜0.05).可见,VEGF受体Flt-1mRNA的表达与VEGF相似.二者均在 I组、II组表达量无差异,具有较高的表达量,随着时间的延长没有明显变化;在III组表达轻微下降,但与I组和II组无显著性差异(P＞0.05);IV组的表达量显著下降(P＜0.05),并且随着时间的延长至逐渐下降.[结论] 反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用.     

The effect of glucocorticoid receptor on level of inflammatory media in rats after critical scald

AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A₂(PLA₂) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA₂ activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.     

Effects of atorvastatin on cardiac myocyte hypertrophy of neonatal rat induced by angiotensinⅡ in vitro and TLR4 expression

AIM: To assess effects of atorvastatin (Ator) on cardiac myocytes (CM) hypertrophy of neonatal rat induced by angiotensinⅡ (AngⅡ) in vitro and Toll like receptor 4 (TLR4) expression for theoretical bases of preventing and treating myocardial hypertrophy.METHODS: CM of neonatal Sprague-Dawley (SD) rats were isolated with trypsin digestion method and those growth-arrested cells were stimulated with 10^-7 mol/L AngⅡ in the presence of various concentrations of Ator.The method of coomassie brilliant blue was adopted to evaluate the protein contents of CM.The changes in β-MHC,AT₁ receptor and TLR4 mRNA expression were observed by RT-PCR.RESULTS: ① AngⅡ increased the protein content of CM and β-MHC mRNA expression significantly,upregulated AT₁ receptor and TLR4 gene expression.② In a dose-dependent manner,Ator inhibited the increases in the protein contents of CM and β-MHC mRNA expression induced by AngⅡ.③ In a dose-dependent manner,Ator downregulated the AT₁ receptor and TLR4 mRNA expression of CM hypertrophy of neonatal rat induced by AngⅡ.CONCLUSION: Ator inhibits CM hypertrophy,downregulates the AT₁ receptor and TLR4 gene expression.     

Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.     

转基因奶牛胚胎移植技术的试验研究

2006年6月至2010年3月以来,北京奶牛中心与中国农业大学合作,在北京奶牛中心良种场开展了转基因牛胚胎移植试验研究。本试验利用中国农业大学培育的转基因母牛作为供体,进行超数排卵生产体内胚胎,将获得的胚胎移植给15～15.5月龄荷斯坦母牛受体。截止2010年3月21日,累计移植转基因奶牛胚胎87枚,移植受体牛87头,妊娠48头,移植妊娠率为55.17%;产犊43头,成活34头,犊牛成活率为79%;经中国农业大学研究人员检测,有14头公牛携带转入目的基因,这14头公牛已全部进入北京市种公牛站饲养。目前,有10头公牛到了试采精月龄,精液经农业部牛冷冻精液质量监督检验测试中心(北京)检测,质量符合国家标准。     

反义抑制ghrelin对VEGF及其受体Fit-1 mRNA表达的影响

［目的］ 探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1 mRNA表达的影响。［方法］ 试验分为4组：Ⅰ组,空白对照组;Ⅱ组,脂质体组;Ⅲ组（SCON）,20 μmol/L正义寡核苷酸组;Ⅳ组（ASCON）,20 μmol/L反义寡核苷酸组。上述各组均在培养24、36、48 h后,采用实时荧光定量方法检测VEGF及其受体Flt-1 mRNA表达情况的变化。［结果］VEGF mRNA在Ⅰ、Ⅱ组表达量无差异,具有较高的表达量,随着时间的延长无明显变化;在Ⅲ组VEGF mRNA 表达略有降低,但与Ⅰ和Ⅱ组间无显著性差异（P＞0.05）;Ⅳ组（反义寡核苷酸组）的VEGF mRNA表达量显著下降（P＜0.05）,并且随着时间延长逐渐下降。VEGF受体FLT-1 mRNA的表达与VEGF相似。［结论］反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用。