Serologic and molecular survey of horses to Coxiella burnetii in East of Iran a highly endemic area

There are few reports about Q fever in horse populations worldwide. This study aimed to detect the C. burnetii infection by serologic and molecular confirmation using commercial ELISA kit and real-time PCR in the East of Iran a region highly endemic. A total of 177 blood samples and 115 vaginal swabs were randomly collected from horses in East of Iran. The sera samples were analyzed for anti C.burnetii Ig G antibodies by a commercial ELISA kit and nucleic acid extraxted from vaginal samples were used to determine the C. burnetii DNA by real-time PCR assay. Antibodies were detected in 5.64 % (10/177) of sera samples and C. burnetii DNA was detected in 7.82 % (9/115) of horse vaginal samples. There was no significant difference in seroprevalence in different sex, age and breed groups. Our study showed that horses could be considered as a mild potential reservoir of C. burnetii which may be effective on horse health status. However, additional studies are needed to assess whether the horse could be considered as a relevant transmission risk indicator for Q fever.     

校园环境噪声监测与评价--以华中师范大学为例

环境噪声是环境质量评价的重要指标。通过噪声监测实验,记录了华中师范大学校园内的噪声污染数据,根据国家标准评估了校园环境噪声状况。试验采用网络剖分法,分别于昼间7:30~10:00和夜间22:00~22:55两个时间段对等效声级进行了测定。实验结果表明:昼间噪声污染更为严重,平均噪声超标率达到26%,夜间噪声污染相对较轻,但噪声超标率也达到19%。校园内不同功能区的噪声来源不同,工程施工产生的噪声是主要的污染源,影响比重达到58%。根据实验结果,校园声环境质量一般,需要对现状提出合理的改进建议。     

Soil protection for a sustainable future: options for a soil monitoring network for Ireland

The increased recognition of the importance of soil is reflected in the UN Post‐2015 Development Agenda with sustainable development goals that directly and indirectly relate to soil quality and protection. Despite a lack of legally binding legislation for soil protection, the European Commission remains committed to the objective of soil protection. However, the achievement of a legally binding framework for soil protection relies on the implementation of a soil monitoring network (SMN) that can detect changes to soil quality over time. As beneficiaries do not pay for the provision of soil information, the options for soil monitoring are limited. The use of existing data sets should be considered first. Using Ireland as an example, this research explored the opportunities for a SMN for Ireland considering three existing national data sets. The options for a SMN are considered in terms of their spatial and stratified distribution, the parameters to be measured and an economic analysis of the options proposed. This research finds that for Ireland, either a 10 or a 16 km² grid interval stratified by land use and drainage class offers the best potential in relation to the spatial distribution of existing data sets to reflect local data at a national level. With existing data, the stratified SIS data using the 16 km² grid offers the best value for money, with baseline costs for analysis, excluding field costs, of between €706 481 and €2.8 million. Acknowledging the impossibility of measuring all parameters with ideal frequency, this study proposes a two‐tier system for optimized monitoring frequency. Parameters must anticipate future policy requirements. Finally, the implementation of a SMN must be accompanied by standardized methods, defined thresholds and action mandates to maintain soil quality within allowable limits.     

分子标记用于赤眼蜂分子监测的研究

通过对松毛虫赤眼蜂（Trichogramma dedrolimi Matsumura）以及玉米螟赤眼蜂（Trichogramma ostriniae Pang et Chen）rDNA-ITS2基因的克隆测序，并联机检索GenBank核酸序列库中其它赤眼蜂的相关序列，利用核酸分析软件找到松毛虫赤眼蜂和玉米螟赤眼蜂ITS2序列中一段有鉴别意义的标志，并据此设计出蜂种的特异PCR引物，实现了松毛虫赤眼蜂和玉米螟赤眼蜂rDNA特异区带的PCR扩增。研究表明该特异区带具有种的特异性。我们认为该分子标记技术可用于目前我国生防中常用的松毛虫赤眼蜂和玉米螟蒌眼蜂的分子监测，如田间种群动态监控和寄生效果评估。     

GI PiCO2: Tissue Specific Monitoring For Improving Patient Outcomes

Improvements in human patient monitoring despite their development in animals, do not always find their way into veterinary clinical use due to financial constraints. Gastrointestinal intraluminal CO₂ partial pressure (Gip₁CO₂) monitoring, however, is not only proving very beneficial in human trauma and critical patient care but is also very likely to become relatively inexpensive. By providing information on the perfusion adequacy of a high risk, critically important tissue, the GI mucosa, GI P₁CO₂ monitoring offers an easily accesible indicator of the efficacy and adequacy of resuscitative interventions. The potential for decreasing morbidity and mortality is enormous. Therefore, the practicing veterinarian should become familiar with GI P₁CO₂ monitoring theory and technology so he or she can be better prepared to incorporate it into practice when in becomes available.     

基于单片机的脉码传输仪设计

以单片机为核心,采用软件、硬件相结合的方法,充分利用单片机的硬件中断资源,实时响应地面的声波逻辑脉冲,分离其接收和发射逻辑。同时该仪器以地面声波逻辑为工作时序,分时采集模拟道数据、脉冲道数据及向地面传输PCM数据。该脉码传输仪既能完成PCM3506的全部功能,又能监测仪器工作环境的温度及测井套管接箍的压力。     

沙尘暴应急监测方法研究

在沙尘暴应急监测方法研究中,通过对总悬浮颗粒物采样仪器及各种采样参数的研究确定和降尘采样器的研制,制定出沙尘暴应急监测方法。     

荒漠化能量与水平衡监测系统的应用

利用卫星遥感技术进行宏观监测是我国荒漠化监测的重要手段,它具有高效经济的特点。本文阐述了中荷合作项目中荒漠化能量与水平衡监测系统的技术方法及主要应用。     

一种监测棉铃虫对Bt杀虫晶体蛋白抗性的技术

研究了以诊断试剂盒监测棉铃虫对苏云金杆菌(Bt)杀虫晶体蛋白(ICP)抗性的技术。将1日龄幼虫在含诊断剂量的饲料中(每毫升饲料含Bt ICP0.01mg)取食7天,以生长至3龄以上的个体作为抗性个体的判断指标;筛选出人工饲料中防腐剂剂量的最佳值为每350毫升饲料中含丙酸2.5ml。经对多个棉铃虫不同抗性种群的验证试验,证明诊断试剂盒检测抗性个体频率较为精确且应用简便。     

等值线制图工具 Surfer在病虫测报上的应用研究

Surfer系统是美国金软公司开发的专门制作等值线的工具。1997年以来,笔者对其运用于稻飞虱等农作物病虫害的监测方面作了探讨和开发。阐述了病虫分布图的绘制和遮盖图编制的过程和方法。