排序方式: 共有13条查询结果,搜索用时 31 毫秒
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东方山羊豆水通道蛋白基因的克隆及初步分析 总被引:1,自引:0,他引:1
根据已知EST片段结合RT-PCR和RACE技术,从东方山羊豆(Galega Orientalis)中克隆到一个水通道蛋白(AQP)基因,命名为GoAQP(GenBank登录号:HM 803185)。测序和生物信息学分析表明,此序列全长1258bp,开放读码框(ORF)870 bp,编码289个氨基酸。GoAQP包含MIP家族信号序列HINPAVT/SFG,高等植物PIP高度保守序列GGANXXXXGY和TGI/TNPARSL/FGAAI/VI/VF/YN。与拟南芥AQP基因家族的系统进化分析表明,GoAQP属于PIP1亚型AQP蛋白。Real-Time PCR检测结果表明,GoAQP基因在根中表达量最高,茎中表达量最少;且受NaCl和PEG胁迫表达上调,NaCl表达呈双峰分布。因此GoAQP基因可能参与了东方山羊豆耐盐性调控。同时成功构建超表达载体pCAMBIA-1302-GoAQP,为转基因验证基因功能创造了基础。 相似文献
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多效唑对百子莲花葶矮化效应的研究 总被引:1,自引:1,他引:0
以3年生盆栽百子莲品种‘Big Blue'为试材,研究不同浓度的多效唑对百子莲花葶生长、细胞形态和生理指标的影响.结果表明,不同浓度的多效唑处理对百子莲花葶生长和细胞纵向生长均有不同程度地抑制作用,且花葶长度随着多效唑浓度的增加而呈下降趋势.其中以400 mg/L的多效唑处理效果最佳,其花葶长度比对照平均缩短了61.1%.另外,由相关性分析得出花葶长度与其上、下部细胞长度极显著正相关,相关系数为0.772和0.806.由切片组织化学分析和生理指标测定结果表明,多效唑处理明显提高了花葶中可溶性蛋白、可溶性糖和淀粉的含量. 相似文献
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百子莲组织培养及植株再生研究 总被引:3,自引:0,他引:3
以百子莲花蕾为外植体,成功建立了其离体再生体系。结果表明:百子莲花蕾在诱导培养基MS+6-BA 4mg/L+NAA 0.1mg/L可分化出不定芽;在MS+6-BA 2mg/L+NAA 0.2mg/L+GA3 0.1mg/L培养基上,百子莲不定芽增殖倍数可达到5.07;百子莲不定芽适宜的生根培养基为1/2MS+NAA 0.1mg/L+AC 0.1g/L,生根率可达100%,且百子莲组培苗移栽1个月后成活率可达到95%以上。 相似文献
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对虾弧菌病发生时临界菌值的研究 总被引:5,自引:0,他引:5
通过对3000多公顷虾池检测496份病虾及水体,均证明致使对虾发生弧菌病、气单胞菌病时的临界菌量值为10~(4.5)~10~5个/ml,当虾池中弧菌数达到10~(4.5)个/ml时,对虾就可能发病,此时就需用药物预防或换水,并证明弧菌菌量与水温、饵料、对虾密度的关系为正相关. 相似文献
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低温胁迫对三种园林植物的生理指标的影响 总被引:2,自引:0,他引:2
选择安息香锦带(Weigela Styriaca),金亮锦带(Weigela florida Goldrush),邱园蓝莸(Caryopteris clandonensis Kew Blue)三种植物的当年生枝条为试材,进行不同梯度的人工低温胁迫。结果表明:随着温度的降低,相对电导率逐渐增大,SOD活性都有不同程度的降低,渗透调节物质可溶性糖和可溶性蛋白总体趋势是逐渐增大。研究结果表明,锦带抗寒性明显大于莸,而金亮锦带的抗寒性要优于安息香锦带。 相似文献
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Jos Gustavo Ramírez‐Paredes Miguel Angel Mendoza‐Roldan Benjamin Lopez‐Jimena Khalid Shahin Matthijs Metselaar Kim D. Thompson David J. Penman Randolph H. Richards Alexandra Adams 《Journal of fish diseases》2019,42(8):1191-1200
Francisella noatunensis subsp. orientalis is a pathogen of tilapia and other warm‐water fish for which no vaccines are commercially available. In this study, a whole cell formalin‐inactivated vaccine was developed for the first time using the highly virulent isolate STIR‐GUS‐F2f7 and the oil‐based adjuvant Montanide? ISA 763A VG. The efficacy of the vaccine was assessed in red Nile tilapia via intraperitoneal (i.p.) injection using homologous experimental infection and correlates of protection such as seral antibody production and bacterial loads in the spleen. For immunization, fish were i.p. injected with 0.1 ml of the vaccine, the adjuvant alone or PBS. At 840 degree days post‐vaccination, all fish were i.p. injected with 4.0 × 103 CFU/fish of pathogenic bacteria. The RPS at the end of the trial was 100% in the vaccinated group with significantly higher survival than in the adjuvant and control groups. The RPS in the adjuvant group was 42%, and no significant difference was seen in survival between this and the PBS group. Moreover, significantly higher antibody titres in the serum and significantly lower bacterial loads in the spleen were detected in the vaccinated fish by ELISA and qPCR, respectively. These findings highlight the potential of autogenous vaccines for controlling francisellosis in tilapia. 相似文献