Insulin action potentiation by 17β-estradiol in cultured C2C12 mytoblasts

AIM:To investigate the effect of 17β-estradiol on insulin action in cultured C2C12 mytoblasts. METHODS:C2C12 mytoblasts were cultured in 35 mm wells of six-well culture plate in an atmosphere of 5% CO₂ at 37℃ in DMEM supplemented with 10% FBS and penicillin/streptomycin(1×10⁵ U/L) to reach 80% confluence. Insulin-resistance C2C12 mytoblasts were obtained by incubating the cells for 24 hours in the presence of a high concentration (5×10^-7 mol/L) of insulin. After treatmented with 17β-estradiol (1 nmol/L and 10 nmol/L, respectively) for 24 hours, C2C12 mytoblasts were performed to measure insulin-stimulated 2-DG uptake and GS, PFK, PK activities. RESULTS:17β-estradiol enhanced the capacity of insulin-stimulited 2-DG uptake, increased the GS, PFK and PK activities and prevented insulin-induced resistance in cultured C2C12 mytoblasts. CONCLUSION:17β-estradiol potentiates insulin action and preventes insulin-induced resistance in cultured C2C12 mytoblasts.