校园环境噪声监测与评价--以华中师范大学为例

环境噪声是环境质量评价的重要指标。通过噪声监测实验,记录了华中师范大学校园内的噪声污染数据,根据国家标准评估了校园环境噪声状况。试验采用网络剖分法,分别于昼间7:30~10:00和夜间22:00~22:55两个时间段对等效声级进行了测定。实验结果表明:昼间噪声污染更为严重,平均噪声超标率达到26%,夜间噪声污染相对较轻,但噪声超标率也达到19%。校园内不同功能区的噪声来源不同,工程施工产生的噪声是主要的污染源,影响比重达到58%。根据实验结果,校园声环境质量一般,需要对现状提出合理的改进建议。     

黑龙江省国有林区脆弱异质性家庭对停伐政策的态度及影响因素研究

基于2016年黑龙江省重点国有林区348户家庭的微观调查数据,实证分析黑龙江省国有林区脆弱异质性家庭对停伐政策的态度及影响因素。采用因子分析与综合评价法将348户职工家庭分为脆弱性家庭和非脆弱性家庭,描述性分析2组样本家庭对停伐政策的态度及认知情况,利用多元有序logit模型分析脆弱性与非脆弱性家庭对停伐政策态度的影响因素。结果表明:非脆弱性家庭对停伐政策的了解程度及执行情况的评价均高于脆弱性家庭,脆弱性家庭相对非脆弱性家庭对停伐政策的认可度较高,但是应对风险冲击的能力相对较低。脆弱性家庭和非脆弱性家庭对停伐政策的态度受户主特征、家庭特征以及政策认知特征变量的显著影响,但脆弱性家庭变量参数估计绝对值相对更大,因而停伐政策对脆弱性家庭有着更为敏感和强烈的冲击。     

Soil protection for a sustainable future: options for a soil monitoring network for Ireland

The increased recognition of the importance of soil is reflected in the UN Post‐2015 Development Agenda with sustainable development goals that directly and indirectly relate to soil quality and protection. Despite a lack of legally binding legislation for soil protection, the European Commission remains committed to the objective of soil protection. However, the achievement of a legally binding framework for soil protection relies on the implementation of a soil monitoring network (SMN) that can detect changes to soil quality over time. As beneficiaries do not pay for the provision of soil information, the options for soil monitoring are limited. The use of existing data sets should be considered first. Using Ireland as an example, this research explored the opportunities for a SMN for Ireland considering three existing national data sets. The options for a SMN are considered in terms of their spatial and stratified distribution, the parameters to be measured and an economic analysis of the options proposed. This research finds that for Ireland, either a 10 or a 16 km² grid interval stratified by land use and drainage class offers the best potential in relation to the spatial distribution of existing data sets to reflect local data at a national level. With existing data, the stratified SIS data using the 16 km² grid offers the best value for money, with baseline costs for analysis, excluding field costs, of between €706 481 and €2.8 million. Acknowledging the impossibility of measuring all parameters with ideal frequency, this study proposes a two‐tier system for optimized monitoring frequency. Parameters must anticipate future policy requirements. Finally, the implementation of a SMN must be accompanied by standardized methods, defined thresholds and action mandates to maintain soil quality within allowable limits.     

导入AGPase基因的转基因可育水稻及其经济性状的研究

以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。     

Feline Ubiquitin Fusion Protein Genes

Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells.     

用台盼兰—姬姆萨染色检测家畜精子顶导反应的研究

本文探讨了用台盼兰—姬姆萨染色检测家畜精子顶体反应的可行性。用肝素或钙离子载体诱发精子顶体反应。根据染色结果将精子分为四类:a)核后帽部不着色或淡青色,顶体不着色或部分紫红色(有顶体反应活精子);b)核后帽部暗青色.顶体部不着色或部分暗红色(有顶体反应死精子);c)核后帽部不着色或淡青色,顶体部紫红色(无顶体反应活精子);d)核后帽部暗青色,顶体部暗紫红色(无顶体反应死精子)。有顶体反应活精子百分率与仓鼠卵穿透率呈强正相关。从而证明台盼兰—姬姆萨染色是检测家畜精子顶体反应的有效手段,并能预测获能处理后精子的受精能力。     

分子标记用于赤眼蜂分子监测的研究

通过对松毛虫赤眼蜂（Trichogramma dedrolimi Matsumura）以及玉米螟赤眼蜂（Trichogramma ostriniae Pang et Chen）rDNA-ITS2基因的克隆测序，并联机检索GenBank核酸序列库中其它赤眼蜂的相关序列，利用核酸分析软件找到松毛虫赤眼蜂和玉米螟赤眼蜂ITS2序列中一段有鉴别意义的标志，并据此设计出蜂种的特异PCR引物，实现了松毛虫赤眼蜂和玉米螟赤眼蜂rDNA特异区带的PCR扩增。研究表明该特异区带具有种的特异性。我们认为该分子标记技术可用于目前我国生防中常用的松毛虫赤眼蜂和玉米螟蒌眼蜂的分子监测，如田间种群动态监控和寄生效果评估。     

牛磺酸在猫营养中的应用

主要综述近年猫的牛磺酸营养,从研究方法来看衡量牛磺酸利用的方法与指标已较完善,对牛磺酸的代谢有了深入的了解,并进一步关注猫肠道菌群影响牛磺酸代谢的机理的研究;初步揭示大豆蛋白和脂肪等营养物质降低牛磺酸利用率的原因;在猫粮加工方面指出不良热处理导致的猫粮中牛磺酸利用率降低的机制,并分析了一些不适合配制猫粮的原料如米糠、大豆蛋白对牛磺酸代谢的影响;以及在猫粮中直接添加晶体牛磺酸的重要性。从文中可以看到,作为必需氨基酸的牛磺酸对于猫的生长繁殖来说十分重要。     

RT-PCR及RFLP分析技术对鸡传染性支气管炎病毒的诊断和S1基因分型的研究

本研究使用分别扩增整个S1糖蛋白基因 (引物A)和S1糖蛋白基因N_端高变区Ⅰ (引物B)的 2对引物对 3个IBV标准株M41、Connecticut、Arkansas及 5个地方分离株 (C60 ,D41A ,D41B ,A112 1,A1171)进行RT_PCR扩增。用引物A时 ,有 5个IBV毒株扩增到目的片段 (172 0bp) ;用引物B时 ,所有 8个IBV毒株均得到与预计大小相符的目的产物 (2 2 8bp)。对 172 0bp的PCR产物用限制性内切酶HaeⅢ进行酶切 ,结果得出 3个不同的RFLP图谱 ,其中M41、Connecticut、D41B具有相同的HaeⅢ酶切图谱。Arkansas和D41A则分别具有互不相同的图谱 ;对 2 2 8bp的PCR产物进行DdeⅠ、RsaⅠ限制性内切酶消化 ,根据它们的RFLP图谱 ,8个IBV毒株可分为 5个基因型。综合 2对引物的PCR产物的RFLP分析结果 ,8个IBV毒株可分为 7个基因型 ,分型的结果与传统的血清学方法吻合。本研究建立的方法和技术具有快速、简单、特异、灵敏等优点 ,为现场流行毒株的定型(基因型 /血清型 )及其S1基因变异的跟踪研究以及更有效防制传染性支气管炎奠定了基础。     

GI PiCO2: Tissue Specific Monitoring For Improving Patient Outcomes

Improvements in human patient monitoring despite their development in animals, do not always find their way into veterinary clinical use due to financial constraints. Gastrointestinal intraluminal CO₂ partial pressure (Gip₁CO₂) monitoring, however, is not only proving very beneficial in human trauma and critical patient care but is also very likely to become relatively inexpensive. By providing information on the perfusion adequacy of a high risk, critically important tissue, the GI mucosa, GI P₁CO₂ monitoring offers an easily accesible indicator of the efficacy and adequacy of resuscitative interventions. The potential for decreasing morbidity and mortality is enormous. Therefore, the practicing veterinarian should become familiar with GI P₁CO₂ monitoring theory and technology so he or she can be better prepared to incorporate it into practice when in becomes available.