狗舌草提取物诱导淋巴细胞性白血病L1210细胞分化的研究

以单猪屎豆碱（MO）、槲皮素（QU）和环磷酰胺（CY）为参照，观察了狗舌草600mL／L乙醇提取物（EX）对淋巴细胞性白血病L1210细胞体外试验的形态变化；利用流式细胞术，从DNA分子水平上检查了EX对L1210细胞各周期相的影响，探讨EX对L1210细胞的分化机理。结果发现，EX能够使L1210细胞向淋巴细胞方向发展；经EX作用24h后，L1210细胞G0＋G1期的百分比较对照组明显升高。提示EX对L1210细胞增殖的抑制作用可能是由于G1期的阻滞所致。     

陆地棉核雄性不育材料在山东不同地区的育性转变及应用研究

对在山东的济南、平度、莱西、烟台种植的几种陆地棉（Ｇｏｓｓｙｐｉｕｍ　ｈｉｒｓｕｔｕｍＬ．）核雄性不育材料的育性表现进行了研究,结果表明：洞Ａ不育系的衍生材料ＭＢ、ＭＡ在不同气候条件下有育性的变化。在济南高度不育,可作为不育系。在其他几个地区表现不同程度的可育。其中ＭＢ在平度有相当好的育性,能正常结铃。而双隐性核不育材料５９Ａ、６２Ａ未能观察到育性变化。本文就核不育材料在棉花杂交制种中的应用前景及需深入研究的问题作了探讨。     

Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.     

不同品系杜洛克后代商品猪胴体性状的比较分析

为研究不同品系杜洛克作为终端父本对其后代商品猪胴体性状的影响,选取214和108头分别由美系和加系杜洛克公猪配种、出生时间相近(±2 d)和体重相近(35±5 kg)的后代三元商品猪进行育肥饲养,结束饲养后开展屠宰试验,测定背膘厚度、眼肌厚度、瘦肉率和肋骨数.结果显示:加系杜洛克商品后代的背膘厚度、眼肌厚度显著(P<0...     

Factors influencing seed reserve utilization during seedling establishment in maize inbred lines

Strong seedlings are essential for high yield. To explore the foundation of strong seedlings, we investigated various factors influencing the conversion and distribution of seed storage reserves during seedling establishment in maize inbred lines. Three maize inbred lines were used to explore the effects of seed size, seed vigor, illumination duration, temperature, water content, and salt concentration of the seedling medium on the utilization of seed storage reserves during seedling establishme...     

Effects of EBV infection on growth and apoptosis of nasopharyngeal carcinoma cell line

AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased (P＜0.01). No apoptotic carcinoma cells were detected and bcl-2 postive cells were 2%~3% respectively in 2 kinds of NPC cells.CONCLUSION: Growth of NPC cells is enhanced by EBV infection and EBV-LMP1 expression, but no influence on expression of bcl-2 and apoptosis of NPC cells.     

西南山地玉米育种新一轮骨干自交系SCML0849的选育与应用

20世纪末,针对西南区21世纪初及可见将来对玉米品种与产业发展的迫切要求,遵循“生态育种”的基本原理,并基于前期“温带种质优良自交系是选育西南玉米强优势杂交种的最重要亲本自交系”的研究结论,定向合成了“REID种质育种用群体”。通过紧密结合常规遗传育种方法与现代分子生物学技术,集成创新了以“配合力早期测定、目标区域与高密度种植鉴定、南北穿梭鉴定选择与钝化光周期反应、区域抗病抗逆特性一票否决”为主要内容的“特定生态区域育种骨干亲本的定向培育”技术体系,提高了优良自交系的选育效率,育成了集高配合力、高抗西南地区玉米病害与多种抗逆性、高产高效等优良性状于一体的玉米优良自交系SCML0849,组配育成了以川单99为代表的一批稳产高产、高抗多抗、资源高效的玉米强优势杂交种并大面积推广应用。     

不同生态区玉米自交系苞叶与穗轴同步发育特性研究

2022-2023年在河南和海南以玉米自交系驻85、驻136、ZM3358为试验材料,研究自交系苞叶与穗轴同步发育特性。结果表明,河南苞叶驻85第1～7 d伸长迅速,速增期与ZM3358一致,低于驻136;穗轴,驻85第1～11 d急剧伸长。海南苞叶ZM3358伸长速增期为13 d,比驻85、驻136延长2～3 d;穗轴ZM3358伸长渐增期最长为19 d,显著高于驻85、驻136。河南苞叶驻85宽度持续变宽,驻136苞叶宽度第11 d开始增幅变缓,ZM3358苞叶宽度速增期最短为7 d;穗轴粗度驻136第1～17 d持续变粗,第19 d稍有下降。海南ZM3358苞叶宽度速增期为13 d,驻85、驻136苞叶宽度速增期均为9 d;驻85穗轴粗度速增期最长为19 d,苞叶与穗轴可保持同步发育特性。     

利用SNP芯片对部分黄淮海玉米自交系遗传多样性分析

利用4 434个SNP标记对47份玉米自交系进行种质遗传多样性和杂种优势类群研究。采用Admixture软件对自交系的遗传结构进行分析,采用Treebest软件对自交系进行聚类分析和类群划分。结果表明,47份自交系分为6个类群,分别为瑞德群、兰卡斯特群、塘四平头群、PB群、旅大红骨群和自330亚群。10个自选系组配的杂交种主要利用的杂种优势模式为PB×塘四平头和瑞德×塘四平头,均为近年来黄淮海地区主要利用的杂种优势模式。