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木本植物组织总RNA提取的要点与原理 总被引:57,自引:1,他引:57
根据提取木本植物组织RNA时所获得的知识和经验,对木本植物组织总RNA提取时所遇到的提取产量低、RNA降解、多酚物质干扰、DNA干扰、多糖干扰、蛋白质干扰及杂质干扰等问题进行了探讨,并提出了具体的解决方法。同时,对各种常用的RNA提取方法进行了分类,并对它们的原理及优劣进行了论述与评价。 相似文献
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Container-grown Ligustrum ovalifolium L. plants were used to determine the influence of nutrient availability on the mobilisation of carbon (C), nitrogen (N) and phosphorus (P) during spring growth. During the spring of the second growing season, plants either received no fertiliser, or were fertilised early (starting 17 days after bud break) or late (starting 3 months after bud break). Nutrient composition of different plant tissues was determined at several times during the second growing season from bud break to the end of elongation. Time of fertilisation did not influence aerial dry weight until flowering was completed. Plants that received no fertiliser or were fertilised late had greater root dry weight than plants that received fertiliser early. Fertilised plants had a second flush of growth after flowering completed. Nitrogen and phosphorus mobilisation occurred from bud break to the end of elongation (3 months). Nutrient mobilisation was effective in each perennial organ: root, trunk, ligneous stems and old leaves. However, the role of old leaves as storage organ was minor compared to evergreen tree leaves. Carbon mobilisation only occurred on the 1st month following bud break, before elongation. C-starch accumulation was observed in unfertilised plants even when the C-starch quantities in early fertilised plants were very low at the end of elongation, mainly in roots. In spite of fertilisation supplies, N and P quantities did not increase after elongation in the early fertilised plants, probably in relation to C insufficiency in roots to sustain N and P absorption and assimilation. By contrast, in late fertilised plants, high C quantities in roots were mobilised for N and P uptake and assimilation, which allowed both second growth flush and storage of N and P in perennial organs. The mobilisation of nutrients before and during elongation revealed the nutritional autonomy of shrubs, which could allow fertiliser use efficiency to be increased and environmental impacts to be minimised by delaying spring fertilisation. Nevertheless, the shrub ability to valorise fertilisation supplies for sustaining growth or nutrient storage restoration required sufficient C-starch quantities in roots. The starch accumulated in roots before late nutrient supply can be used for these objectives. 相似文献
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内蒙古木本植物区系的研究 总被引:3,自引:0,他引:3
刘果厚 《内蒙古林学院学报》1998,20(3):97-104
内蒙古共有木本植物483种(包括种下分类单位,野生种418种,栽培种65种)隶属于52科,139属,其中乔木132种,灌木及半灌木和小半灌木351种,这些植物中,裸子植物3科7属31种,被子植物49科132属452种,在被子植物中双子叶植物48科131属451种,单子叶植物1科1属1种,本文对418种野生木本植物进行了区系统计和分析,结果表明,该区系属于温带性质,地理联系广泛,水平替代现象明显,交 相似文献
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利用甘肃民勤荒漠草地生态系统国家野外科学观测研究站1975—2013年气象资料,与当地乔木沙枣、二白杨、胡杨、小枣、家杏、桑树6种乔木物候观测资料,通过5年时间段统计物候发生日的偏离程度,统计春季物候年度展叶期、开花期变化趋势,分析物候变化对气温变化的响应关系。结果表明,近39年研究区春季平均气温以0.053℃/a的速度升高,荒漠植物的多种物候期出现同步性的提前;39年春季物候提前了7.6~12.1 d,即气温每提升1.0℃,可导致春季物候期提早2.1~5.8 d,秋季落叶期平均推迟3.8 d。 相似文献
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