TDP穴区照射调整奶牛分娩前后血液流变性的研究

 健康妊娠奶牛20头随机分为照射组和对照组各10头。照射组奶牛分别于分娩前10～3d和产后1～3d,用TDP辐射仪交替照射百会和后海穴区,每天1次,60min。奶牛分别于分娩前20、15、10、5、3、1d,1～4h和产后1～4h、1、3、15d,颈静脉采血样。检测血样的ηb80s-1,ηb20s-1,ηp,PCV,ηr,RI,AI和血浆的Glu,TP,FG的含量。与对照组比较,结果:TDP穴区照射对奶牛分娩前和后一定时期内的ηb80s-1,ηb20s-1和ηp具有一定调整作用;调整和改善分娩过程中的血糖含量和血液的流动性与变形性,减少血液丢失等,从而提高机体分娩应激反应综合适应能力。其作用机理可能是TDP辐射红外热能的经、穴感应调整作用和机体产生的生物能量效应等的综合。     

Influence of Tangshen formula on endothelial function and hemorheology in diabetic nephropathy rats

ATM: To explore the influence of Tangshen formula (TS) on endothelial function and blood rheology in diabetic nephropathy (DN) rats. METHODS: The DN rat model was established by intravenous injection of low-dose (30 mg/kg) streptozotocin (STZ) after having the high-fat/high-glucose diets for one month. The animals were divi-ded into DN model group, TS group and valsartan group. Fasting blood glucose (FBG), serum total cholesterol (TC), serum triglyceride (TG), renal cortex blood flow and hemorheology were monitored. The content of von Willebrand's factor (vWF) and plasminogen activator inhibitor-1 (PAI-1) in the serum was determined by ELISA. RESULTS: Compared with normal group, FBG,TC,TG, vWF and PAI-1 were increased in DN model group (P<0.05), and no significant difference of FBG was observed. Compared with normal group, plasma viscosity, Casson viscosity, whole blood high/medium/low-shear viscosity, erythrocyte aggregation index, erythrocyte rigidity index and erythrocyte electrophoresis time were increased, and erythrocyte deformation index was decreased in DN model group (P<0.01). Compared with DN model group, plasma viscosity, Casson viscosity, whole blood high/medium/low-shear viscosity, erythrocyte aggregation index, erythrocyte rigidity index and erythrocyte electrophoresis time were decreased (P<0.05), but there was no significant difference for erythrocyte deformation index in TS group. Compared with normal group, the renal cortex microcirculation blood flow in DN model group was significantly decreased. Compared with DN model group, the renal cortex microcirculation blood flow was significantly increased in TS group (P<0.05), and no significant change in valsartan group was found.CONCLUSION: Tangshen formula plays a protective role in the kidney of diabetic rats by improving the blood rheology and endothelial function, thus ameliorating the renal cortex microcirculation blood flow in experimental diabetic rats.     

Agkistrodon halys venom PCA improves hemorheology in rats with coronary artery microthrombosis

AIM: To explore the effect of protein C activator (PCA) from Wannan Agkistrodon halys venom on hemorheology in the rats with coronary artery microthrombosis(CAM). METHODS: Fifty SD rats were randomly divided into sham operation group, CAM group, low dose (0.5 mg/kg), medium dose (2 mg/kg ) and high dose (8 mg/kg) of PCA treatment groups. The model of CAM was induced by injecting sodium laurate at dose of 1.0 mg/kg with the concentration of 10 g/L from aortic root to left ventricle. Thrombelastogram was obtained by thromboelastography instrument system. The content of endothelin-1(ET-1) and P-selectin, and the activity of creatine kinase-MB (CK-MB),lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) in plasma were determined by ELISA. The protein expression of myocardial P-selectin and TNF-α was detected by Western blotting. The structural changes of the myocardial cells and interstitial tissues were observed under microscope. RESULTS: Compared with CAM group, clotting time and clot formation time significantly prolonged in high-dose PCA group. The alpha angle, maximum amplitude, coagulation index, and the levels of ET-1, P-selectin, CK-MB, LDH and AST in plasma were decreased. The protein expression levels of P-selectin and TNF-α in myocardial tissues were down-regulated (P<0.05). Cardiac histological observation showed that coronary microthrombi in medium-dose and high-dose PCA groups were not found. CONCLUSION: Agkistrodon halys venom PCA inhibits the formation of coronary microthrombi, down-regulates the expression of P-selectin and TNF-α, improves the abnormal hemorheology after microthrombi, and effectively protects myocardial cells.     

黄牛寒证的血液动力流变学初探

首次应用无创检测技术对１８例寒证黄牛的２３项血液动力流变学指标进行了检测：ＰＲ，ＳＶ，ＣＯ，ＳＩ，ＣＩ，ＶＰＥ，ＬＶＷＩ，ＦＥＫ和ＡＣ等心血管动力学指标均有不同程度的降低，其中ＰＲ，ＣＯ，ＣＩ和ＦＥＫ变化显著（Ｐ＜０．０５），η，ＭＨＲ，ＭＲＴ，ＭＳＴ等流变学指标变化明显（Ｐ＜０．０５—０．０１），呈现微循环瘀滞和高粘血症。     

碟脉灵注射液对急性血瘀模型大鼠血液流变学的影响

采用大鼠皮下注射0.1%肾上腺素加冰水中浸泡5min复制急性血瘀模型,观察碟脉灵注射液对急性血瘀模型大鼠血液流变学的影响。结果表明,碟脉灵注射液2.5、5、10ml/kg可使急性血瘀模型大鼠全血黏度、血浆黏度、血浆纤维蛋白原浓度、血沉、红细胞压积、红细胞电泳时间、红细胞聚集指数及红细胞刚性指数均明显降低。提示其对急性血瘀模型大鼠血液流变学的异常变化有明显改善作用,这有利于防止急性心肌梗死及脑梗死时的高黏状态,预防血栓形成。     

虫草多糖对气虚模型大鼠血液流变学指标的影响

[目的]探讨虫草多糖对气虚模型大鼠血液流变学的影响。[方法]以不同剂量的虫草多糖为药物,灌胃"气虚"造模大白鼠,研究虫草多糖对气虚模型大白鼠血液流变学指标的影响。[结果]对游泳劳损造成的大鼠"气虚",在高切变率(230 s-1)、中切变率(115和46 s-1)及低切变率(5.75 s-1)下,高、低剂量的虫草多糖均能显著降低"气虚"造模大白鼠的全血粘度和血浆粘度,高剂量虫草多糖能显著降低造模大白鼠的红细胞压积。[结论]虫草多糖具有较好的益气药理作用。     

蹄变形奶牛血液流变学的研究

为了更深入探讨奶牛变形蹄的发病机理及对奶牛变形蹄的预防和治疗提供更科学的理论依据。选择长沙地区20头中国荷斯坦牛，系统检测了变形蹄和正常蹄奶牛在夏季（6月份）和冬季（12月份）血液流变学主要指标。夏季时，1S-1、3S-1、5S-1、10S-1、30S-1、60S-1切变率下，变形蹄奶牛全血粘度高于正常蹄奶牛，有显著差异（P〈0．05）。变形蹄奶牛低切全血还原粘度极显著高于正常蹄奶牛（P〈0．01）。变形蹄奶牛红细胞压积高于正常蹄奶牛，差异显著（P〈0．05）。冬季时，5S^-1、10S^-1、30S^-1、200S^-1切变率下，变形蹄奶牛全血粘度高于正常蹄奶牛（P〈0．05）。变形蹄奶牛血浆粘度高于正常蹄奶牛，有显著差异（P〈0．05）。变形蹄奶牛红细胞沉降率较快，也有显著差异（P〈0．05）。变形蹄奶牛与全血粘度增高和红细胞聚集性增强具有相关性。     

内毒素休克时山羊血液流变性的变化及山莨菪碱对其影响

本文通过给山羊静注大肠杆菌内毒素诱导内毒素休克,探讨内毒素休克时血液流变性的变化规律,并观察山莨菪碱（６５４－２）对其影响。结果表明,山羊内毒素休克时,低切率全血比粘度（ＬＢＶ）和低切率全血还原比粘度（ＬＢＲＶ）明显升高（Ｐ＜０．０５）,血浆比粘度（ＰＶ）和红细胞聚集指数（ＡＩ）均显著增高（Ｐ＜０．０１,Ｐ＜０．０５）,红细胞变形能力（ＲＣＤ）显著下降（Ｐ＜０．０５,Ｐ＜０．０１）,当静注内毒素前１０ｍｉｎ给予６５４－２（２．５ｍｇ／ｋｇ）山羊的ＬＢＶ和ＬＢＲＶ在１～５ｈ显著高于对照组,５ｈ前ＰＶ和ＡＩ值比对照组有明显升高,第７ｈ后与对照组间无明显差异（Ｐ＞０．０５）,且显著低于休克组（Ｐ＜０．０５）,其ＲＣＤ亦趋于正常。提示山羊内毒素休克时血液流变参数明显改变,血液粘度增加,红细胞聚集加剧,而应用６５４－２具有显著改变血液状态,缓解微循环障碍发生。     

黄牛实验性瘤胃切开术血液流变学主要指标变化规律研究

为了探讨黄牛瘤胃手术血液流变学主要指标的变化规律和指导术后治疗用药 ,对 1 0头健康成年黄牛瘤胃手术前后 2周的血液流变学 ,即全血比粘度 ( ηb) ,血浆比粘度 ( ηP)、红细胞压积 ( HCT)、全血还原比粘度 ( ηr)、血红蛋白含量 ( HB)、红细胞沉降速度 ( ESR)、红细胞聚集指数( AI)和纤维蛋白质含量 ( FG)等 8项指标进行了系统检测。结果表明 :1 0头黄牛在瘤胃手术后一周内其 ηb、ηP、ηr、ESR、HCT、AI和 FG值比术前极明显增高 ( P<0 .0 1或 P<0 .0 5 ) ,且 HB值也有明显升高 ( P<0 .0 5 )。黄牛因外科手术中紧张不安 ,机体的应激以及术中和术后血液的流失 ,可对血液流变性产生影响 ,此实验对外科手术前后的处理输液用药具有重要意义     

流产母马血液流变学研究

[目的]研究流产对母马血液流变学的影响。[方法]分别检测正常非孕母马、正常怀孕母马、流产母马、正常分娩母马的血液流变学指标,并作比较。[结果]正常非孕母马与正常怀孕母马的各项血液流变学指标无明显差异（P〉0.05）。正常分娩母马的全血比黏度、全血还原比黏度、血浆比黏度、血细胞压积、红细胞电泳时间均显著高于正常非孕母马（P〈0.001）。而流产母马的各项血液流变学指标均显著高于正常分娩母马（P〈0.001）。[结论]母马流产后血液流变学存在异常。