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银杏叶内酯含量的变化规律研究 总被引:7,自引:1,他引:6
对不同产区、不同树龄、不期的200多个银杏叶样品,用HPLC法检测其银杏内醌和白果内酯的含量。发现用于生产银杏叶提取物(GBE)的原料银杏叶中萜内酯含量差异很大,它是影响GBE质量和成本的关键;银杏幼龄(1-6年生)实生苗叶萜内酯含量高,一般0.30-0.50%,大树叶含量低,一般〈0.10%;我国各主要银杏产我都有萜内酯含量≥0.40%的优质银杏叶;不同地区有不同的最要叶期。 相似文献
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提高银杏悬浮培养细胞内酯合成的研究 总被引:8,自引:0,他引:8
对12个不同品种的银杏树幼叶及其诱导的愈伤组织进行银杏内酯含量测定, 发现不同基因型的银杏内酯含量存在较大差异: 圆铃和金坠两个品种含量最高, 分别达0. 43%和0. 41%; 七星果品种含量最低, 为0. 13%; 金坠品种幼叶愈伤组织中银杏内酯含量最高, 为0. 0334%; 有3 个品种的幼叶愈伤组织未检测到银杏内酯。选取银杏内酯含量高、生长旺盛的愈伤组织在B5 液体培养基中进行了细胞悬浮培养,研究多种生长因子对细胞生长和银杏内酯合成的影响。结果表明, 在150 mL 培养瓶中装50 mL 培养液, 接种量为30~ 40 g/ L, 培养液初始pH 为5. 8, 3 000~ 4 000 lx 的强光照, 以30 g / L 蔗糖和15 g/ L 葡萄糖为碳源最有利于细胞生长和银杏内酯的合成。用高效液相色谱检测结果显示, 银杏圆铃品种的悬浮培养细胞中银杏内酯B 最高含量可达细胞干样质量的0. 0758%。 相似文献
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[目的]测定0.6;苦内酯水剂和1.5;除虫菊素水乳剂对棉蚜的防治效果,以用于有机棉田棉蚜的防治.[方法]室内试验测定0.6;苦内酯水剂和1.5;除虫菊素水乳剂对棉蚜的LD50和LC50,并进行田间防效测定.[结果]0.6;苦内酯水剂和1.5;除虫菊素水乳剂对棉蚜在处理24的LC50值分别为430.064 1和473.1395 mg/L;LD50值分别为0.0001和0.000 3 μg/g,[结论]田间药效试验表明1.5;除虫菊素水乳剂持效性较好,施药7 d后的防效为80.13;,0.6;苦内酯水剂的持效期较短药7 d后的防效为43.72;. 相似文献
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AIM: Studying the relation between telomerase activity and chronic myelocytic leukemia(CML) courses by detect telomerase activity(TMA) of mononuclear cells of bone marrow samples in different CML phases. METHOD: Using telomerase PCR-ELISA method. RESULTS: Telomerase activity of normal bone marrow is 9.85±0.68, CML chronic phase is 24.48±12.73 which is significantly higher than that of normal bone marrow(P<0.05). CML accelerated phase TMA is 76.76±21.84 and blast crisis is 90.62±25.41, both are significantly higher than that of chronic phase(P<0.001, P<0.001)but there is no significant difference between accelerated phase and blast crisis(P>0.05), at remisson TMA is 18.12±6.27 which is still higher than normal group(P<0.05).CONCLUTIONS: Telomerase activity in different phases of CML is significantly higher than normal bone marrow group. When telomerase is extremely activated , it means that CML patients were entering the accelerated phase. Telomerase activity might be used as an useful new marker in detecting and curing CML. 相似文献
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