全文获取类型
收费全文 | 163篇 |
免费 | 3篇 |
国内免费 | 15篇 |
专业分类
林业 | 8篇 |
农学 | 17篇 |
12篇 | |
综合类 | 63篇 |
农作物 | 21篇 |
水产渔业 | 12篇 |
畜牧兽医 | 24篇 |
园艺 | 21篇 |
植物保护 | 3篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 3篇 |
2012年 | 7篇 |
2011年 | 7篇 |
2010年 | 13篇 |
2009年 | 6篇 |
2008年 | 9篇 |
2007年 | 15篇 |
2006年 | 9篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 12篇 |
2002年 | 4篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 2篇 |
1995年 | 5篇 |
1994年 | 9篇 |
1993年 | 1篇 |
1992年 | 7篇 |
1991年 | 8篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1986年 | 1篇 |
排序方式: 共有181条查询结果,搜索用时 15 毫秒
1.
利用组织培养技术向普通小麦导入簇毛麦抗白粉病基因的研究 总被引:21,自引:4,他引:21
对硬粒小麦(T.durum)-簇毛麦(H.villosa)杂种幼胚和F#-1幼穗诱导的愈伤组织进行秋水仙素处理获得硬-簇双二倍体TH1,TH1W,TH2W,TH3,TH3W。平均加倍率为78.8%,最高为94.1%。通过与普通小麦(T.aestivum)回交,幼胚和花药培养,得到了6D/6V异代换系94G22-1,94G25-1,94G32-1,94G33-1。谷草转氨酶(GOT)和酯酶(EST)同工酶可作为这些材料的生化标记。RAPD分析中,引物OPAN3可作为其分子标记引物。 相似文献
2.
本实验用聚丙烯酸胺凝胶电泳(PAGE)方法,对塔里木河大狗头鱼(学名:叶尔羌鼓鳔鳅)六种不同组织(骨骼肌、心、肝胰、肾、肠、鳃)中LDH同工酶进行了分析研究,结果表明,LDH同工酶具有组织特异性,其中骨骼肌中含有五种LDH同工酶,即LDH1、LDH2、LDH3、LDH4、LDH5,心、肝胰和肾含有LDH1、LDH2、LDH3,鳃有LDH1和LDH4,肠有LDH4。 相似文献
3.
[目的]探索一套适合于棉花纤维蛋白的双向电泳技术,为研究棉花纤维发育的蛋白质组学奠定基础.[方法]以陆地棉徐州142为材料,比较了三种不同蛋白质提取方法以及不同裂解液组成对双向电泳的影响,并在蛋白上样量和染色方法等方面进行了探索与优化.[结果]利用饱和酚-甲醇醋酸铵法提取的棉花纤维蛋白,其蛋白含量较高,且SDS-PAGE电泳条带清晰;样品溶解于含有硫脲和CHAPS的裂解液中,其蛋白溶解度增加;不同的蛋白上样量比较结果表明,第一向等电聚焦固相pH梯度胶条(7 cm)的最适上样量为300 μg;同时,经改良后的考马斯亮蓝染-脱色后,2-D图谱的背景清晰,分辨蛋白斑点数增加.[结论]研究建立了一套适用于棉花纤维蛋白总蛋白质组分析的双向电泳方法. 相似文献
4.
[目的]为探究克氏原螯虾的食用安全性以及哈夫病的致病因素等提供检测方法。[方法]以孝感市不同水域中生活的克氏原螯虾为材料,对其主要组织的LDH同工酶进行电泳分析。[结果]2处水域的pH、总磷、总氮和化学需氧量存在差异,2处水域中克氏原螯虾的鳃、心脏和肌肉的LDH同工酶酶谱也存在差异。B水域(流水)中克氏原螯虾的酶带数目比A水域(静水)多。从Ldh-5同工酶染色深浅可以看出,静水虾肝胰腺、心脏和眼球的Ldh-5同工酶活力比流水虾的大,而肌肉和腮则是流水虾的Ldh-5同工酶活力较大。[结论]2处水域克氏原螯虾组织LDH同工酶的差异是由水质差异引起的。 相似文献
5.
WU Wei-jiang LI Xiao-hong REN Xian-da LUO Yu-hong ZHOU Qi-jing LIANG Xiao-yu 《园艺学报》2003,19(8):1074-1078
AIM: To study the mechanism of the effect of NSAIDs on apoptosis in mice hepatoma at anti-inflammatory doses. METHODS: Kunming breed mice were inoculated subcutaneously in the flank with mice hepatoma H22 cell line. The effects of ibuprofen, indomethacin, and nimesulide on apoptosis were determined by using electron microscopy, agarose gel electrophoresis, flow cytometry, and Western blot analysis of the expression of c-myc, bcl-xL and bcl-2 proteins. RESULTS: NSAIDs induced apoptosis of mice implanted hepatoma, which includes the morphological changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA. The apoptotic index was increased to 15%±1.0%, 29.7%±1.5%, 46.3%±3.5% from 3.3%±0.6% by detecting Sub-G1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2 and bcl-xL were significantly reduced by treatment with nimesulide. Ibuprofen and indomethacin decreased bcl-2 expression but increased bcl-xL expression. C-myc wasn't changed in these groups. CONCLUSION: These results suggest that NSAIDs induces apoptosis of mice hepatoma, which may be due to their regulation on the expressions of bcl-2 family genes. 相似文献
6.
7.
8.
?sa Lundberg Anna Aspán Ann Nyman Helle Ericsson Unnerstad Karin Persson Waller 《Acta veterinaria Scandinavica》2014,56(1):2
Background
Staphylococcus aureus is an important cause of clinical mastitis in dairy cows worldwide. The cure rate after antimicrobial treatment of clinical S. aureus mastitis is very variable due to both cow and bacterial factors. Studies have shown that bacterial genotype might affect short-term bacteriological and clinical cure, but the long-term outcome has been less studied. The objectives of this study were to investigate associations between bacterial genotype and long-term outcome of veterinary-treated clinical mastitis (VTCM) caused by S. aureus during a follow-up period of 120 days and to study genotype variation among Swedish S. aureus isolates. S. aureus isolates from cases of VTCM were genotyped by pulsed-field gel electrophoresis. Long-term outcome measurements used were somatic cell count (SCC), additional diagnoses of VTCM, milk yield and culling. Isolates were classified into clusters (>80% similarity) and pulsotypes (100% similarity). Clusters and pulsotypes were grouped according to occurrence. Multivariable mixed-effect linear regression models including cow and bacterial factors with possible influence on SCC or milk yield were used to calculate differences in SCC or milk yield between groups. Additional outcome measures were calculated using a test of proportions.Results
The isolates (n = 185) were divided into 18 clusters and 29 pulsotypes. Two pulsotypes were classified as common, and were found in 64% of the cases of VTCM. Remaining isolates were classified as less common or rare pulsotypes. The distribution was similar at cluster level. Outcome was calculated from follow-up data on 111 cows. Significantly lower SCC during the follow-up period was found in cows infected with common clusters compared to in cows infected with less common/rare clusters. The proportion of cows with SCC <200 000 cells/ml during the whole follow-up period was significantly higher in the group common clusters than in the group less common/rare clusters. Bacterial genotype did not influence the other outcome parameters.Conclusions
In Sweden, two S. aureus pulsotypes, identified in about 64% of clinical S. aureus cases, were widespread. Cows infected with the common genotypes had significantly lower SCC during 120 days after treatment compared to cows infected with less common or rare genotypes. 相似文献9.
G. Rossi F. Ibba S. Meazzi A. Giordano S. Paltrinieri 《Veterinary journal (London, England : 1997)》2014,199(1):143-149
This study was designed to determine if the activity of paraoxonase (PON1), an antioxidant enzyme that works as a negative acute phase reactant, is a better predictor for the clinical recovery of leishmaniotic dogs receiving standard treatments compared with inflammatory markers such as C reactive protein (CRP) and electrophoretic fractions. For this purpose we tested 20 healthy dogs (controls) and 39 leishmaniotic dogs classified as sick (group A, n = 23) or severely sick (group B, n = 16) and tested at admission and after 3, 7, 14, 21, 28, 35 and 42 days.At admission, CRP and electrophoresis were altered in both groups, while PON1 activity was abnormal only in group B. There were no differences related to the outcome (mortality, complications or time of recovery). PON1 activity normalized in about 2 weeks in dogs that had abnormal values at admission and a final positive outcome; CRP normalized in 4–6 weeks and electrophoretic fractions were still altered after 6 weeks. The results show that, at admission, inflammatory markers did not predict the outcome of leishmaniasis. PON1 activity decreased only in some dogs with systemic inflammation but not in those with mild leishmaniasis: when decreased, PON1 normalized earlier than other markers in dogs that responded to treatment. This finding most likely depends on the rapid decrease in oxidative phenomena. PON1 activity should therefore be tested on admission: if low values are recorded, severe inflammation may be suspected and PON1 measurement may be repeated during treatment to early identify responsive dogs. 相似文献
10.
用不同基因型的冬小麦品种花药在W142培养基(改进的W14培养基)上获得较高的出愈率,但不曲因型之间仍存在着显著的差异。用Pharmacia公司PhastSystem电泳仪对不同基因型小麦花药及其愈伤组织蛋白质进行等电聚焦电泳和SDS-聚丙烯酰胺凝胶电泳分析,观察到不同基因型花药蛋白质之间存在着差异,并且初步认为这种蛋白质差异与出愈率有相关性。不同基因型花药愈伤组织之间观察不到明显的带型区别,但它 相似文献