垂直轴风力机翼型涡流噪声的数值分析

为了了解垂直轴风力机翼型涡流噪声特性,以LUT翼型为研究对象,首先利用Fluent进行流场分析,流场计算选用DES湍流模型,再结合Lighthill声类比方法计算翼型周围声场,将数值模拟计算得到的气动特性相关数据与该翼型的风洞试验结果作对比分析,同时分析了不同攻角对该翼型气动噪声特性的影响,最后研究了在攻角为8°时不同雷诺数对该翼型的声压级指向性特征影响.结果表明:数值计算所得气动数据与风动试验数据拟合良好,建立的仿真模型、网格质量和边界条件合理有效;随攻角增加,翼型涡脱落从尾缘向前缘推进,同时涡流脱落强度增大,气动噪声增强;随着雷诺数的增加,翼型四周声压级先增加后减小;雷诺数与声压级关联较大,控制叶片雷诺数有助于降低叶片噪声,为该翼型适用于低噪声垂直轴叶片提供理论基础.     

AES和DES的效率及安全性比较

运用C和C＋＋对数据加密标准（Data Encryption Standard,DES）和高级加密标准（Advanced Encryption Standard,AES）进行了实现,并就两者的效率和安全性进行了比较,证明了AES算法的优越性.     

产IAA菌株的UV和DES诱变筛选及培养条件优化

为了得到高产吲哚-3-乙酸(IAA)菌株,采用紫外(UV)诱变和硫酸二乙酯(DES)诱变的方法,对从烟草根际土壤中分离得到的一株产IAA并具有溶有机磷性状的促生菌进行诱变选育,并对获得的目标菌株的发酵培养条件进行正交优化。结果表明,菌株经UV诱变和DES诱变均能有效提高其IAA产量。诱变条件为 UV(15 W,30 cm)照射1 min或2 mg·mL^-1 DES处理30 min时,得到1株高产IAA的菌株UV19,其IAA产量为33.77 mg·L^-1,是出发菌株产量的299.48%。菌株UV19经10代继代,其产IAA能力和溶有机磷性状稳定遗传。菌株UV19产IAA培养发酵优化条件为10%装瓶量、初始pH值8、培养温度25℃、接种量3%、培养时间96 h、含500 mg·L^-1色氨酸的LB培养基,优化后IAA产量高达75.47 mg·L^-1,为优化前的2.23倍。本研究结果为菌肥及生物法生产IAA提供了基础材料及技术方案。     

Seven New and Two Known Lipopeptides as well as Five Known Polyketides: The Activated Production of Silent Metabolites in a Marine-Derived Fungus by Chemical Mutagenesis Strategy Using Diethyl Sulphate

AD-2-1 is an antitumor fungal mutant obtained by diethyl sulfate mutagenesis of a marine-derived Penicillium purpurogenum G59. The G59 strain originally did not produce any metabolites with antitumor activities in MTT assays using K562 cells. Tracing newly produced metabolites under guidance of MTT assay and TLC analysis by direct comparison with control G59 extract, seven new (1–7) and two known (8–9) lipopeptides were isolated together with five known polyketides 10–14 from the extract of mutant AD-2-1. Structures of the seven new compounds including their absolute configurations were determined by spectroscopic and chemical evidences and named as penicimutalides A–G (1–7). Seven known compounds were identified as fellutamide B (8), fellutamide C (9), 1′-O-methylaverantin (10), averantin (11), averufin (12), nidurufin (13), and sterigmatocystin (14). In the MTT assay, 1–14 inhibited several human cancer cell lines to varying extents. All the bioassays and HPLC-photodiode array detector (PDAD)-UV and HPLC-electron spray ionization (ESI)-MS analyses demonstrated that the production of 1–14 in the mutant AD-2-1 was caused by the activated production of silent metabolites in the original G59 fungal strain. Present results provided additional examples for effectiveness of the chemical mutagenesis strategy using diethyl sulphate mutagenesis to discover new compounds by activating silent metabolites in fungal isolates.     

Rare Chromones from a Fungal Mutant of the Marine-Derived Penicillium purpurogenum G59

Three new and rare chromones, named epiremisporine B (2), epiremisporine B1 (3) and isoconiochaetone C (4), along with three known remisporine B (1), coniochaetone A (5) and methyl 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (6) were isolated from a mutant from the diethyl sulfate (DES) mutagenesis of a marine-derived Penicillium purpurogenum G59. The structures of 2–4 including the absolute configurations were determined by spectroscopic methods, especially by NMR analysis and electronic circular dichroism (ECD) experiments in conjunction with calculations. The absolute configuration of the known remisporine B (1) was determined for the first time. Compounds 2 and 3 have a rare feature that has only been reported in one example so far. The compounds 1–6 were evaluated for their cytotoxicity against several human cancer cell lines. The present work explored the great potential of our previous DES mutagenesis strategy for activating silent fungal pathways, which has accelerated the discovery of new bioactive compounds.     

硫酸二乙酯诱变苜蓿愈伤组织抗寒生理的研究

对诱导的苜蓿Medicago sativa愈伤组织进行不同浓度的硫酸二乙酯(DES)诱变处理,确定其半致死浓度,在-7℃低温下进行筛选,以获得抗寒性突变体;进行了相对电导率、游离脯氨酸含量、过氧化物酶的活性等抗寒性生理指标的测定.结果表明,苜蓿愈伤组织经DES处理,DES的半致死浓度为0.3%,愈伤组织的抗寒性增强.     

清真牛羊肉溯源系统中 QR Code 二维码的应用研究

建立清真牛羊肉溯源系统可以对肉品生产运销过程中的危害物及穆斯林禁忌物进行有效控制,提高肉品质量安全。首先阐述了QRCode二维码的基本特性,设计了清真牛羊肉溯源系统的总体框架,实现了溯源系统QR码的生成,并利用DES算法对QR码进行加密,最后完成了QR码识别和溯源查询功能。建立基于QR码的清真肉品溯源系统,一方面保障了消费者对产品的知情权,另一方面提高了内品的安全性与可靠性。     

动物性食品中己烯雌酚残留量检测方法评定

用于检测动物性食品中己烯雌酚残留量的常用方法有放射免疫测定(RIA)、酶免疫测定(EIA)、气相色谱(GC)、高效液相色谱(HPLC)和薄层色谱(TLC)。比较这几种方法,认为RIA法测定的灵敏度较高,测定肝脏中DES的最低检出限可达0.3μg/kg,但对放射防护的要求较高,只能在专门实验室进行。GC法需增加衍生化步骤,检出限为2～10μg/kg。HPLC法用电化学检测器的最低检出限是0.1～0.2μg/kg。TLC法用硅胶作铺板,其最低检出限是0.1μg/kg。EIA法灵敏度高、特异性强,测定中华鳖肌肉中DES的最低检出限0.02μg/kg,是目前用于检测大量样品的首选方法。     

纤维素酶高产菌株的诱变育种

采用黑曲霉LH24为出发菌株,经紫外线和硫酸二乙酯诱变处理,选育出一株生产菌LH2466.在适宜条件下,LH2466产纤维素酶平均为18910 U.g-1.     

攻破“一个在微机上实现的密码系统”

本文指出了文［１］提出的密码系统存在的问题，利用密钥穷举攻击攻破了该系统。