Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro

AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.     

Effects of the juice of Fructus Hippophae (JFH) on thrombocytopenia induced by cyclophosphamide in rats

AIM:To investigate the effects of the juice of fructus hippophae(JFH) on thrombocytopenia in rats.METHODS:Forty-eight wistar rats were divided into control group, model group, Yixuesheng-Jiaonang (YSJN) group and JFH group. Rats were injected intraperitoneally with cyclophosphamide (CTX, 30 mg/kg) or saline once a day for 3 days. And then, Saline, YSJN or JFH was administered (ig) once a day for 11 days. On the second, fouth, sixth and eighth day, the thrombocyte were counted, the mean platelet volume (MPV) and PDW were examined. On the eighth day, the cAMP, cGMP in platelets and platelet aggregation function were also examined.RESULTS:JFH could shorten the time of blood coagulation, increase the count of platelet, and enhance the platelet aggregation function. The experiment also showed that the JFH could decrease cGMP content in platelet.CONCLUSION:JFH could enhance blood coagulation and quality of platelet and prevent thrombocytopenia induced by cyclophosphamide in rats.     

中药“增免散”对免疫抑制鸡免疫功能的影响

将300只1日龄雏鸡随机分成正常对照组、中药“增免散”组、环磷酰胺组、环磷酰胺组 左旋咪唑组、环磷酰胺 “增免散”组,6日龄用鸡新城疫LaSota疫苗免疫点眼滴鼻,14、21、28、35日龄测定鸡血液ND抗体效价、免疫器官指数、细胞免疫功能、红细胞免疫粘附功能等,探索中药“增免散”的免疫增强机理。结果表明:中药“增免散”不但能显著提高Lasota活苗接种雏鸡的特异性HI抗体水平,延长其持续时间,能促进雏鸡免疫器官的生长发育,能显著增高雏鸡外周血T淋巴细胞ANAE阳性率,并对雏鸡红细胞免疫粘附功能具有显著促进作用,而且对环磷酰胺的免疫抑制有部分拮抗作用。     

沙枣多糖对免疫抑制小鼠的调节作用

[目的]研究沙枣多糖(EAPS)对免疫抑制小鼠免疫功能的影响。[方法]以环磷酰胺(Cy)作免疫抑制剂建立小鼠免疫抑制模型,进行免疫脏器指数的检测、碳粒廓清试验、血清溶血素形成和迟发型超敏反应,探讨沙枣多糖对免疫功能低下小鼠的影响。[结果]沙枣多糖能明显提高正常小鼠脾脏指数、胸腺指数及免疫抑制小鼠脾脏指数;增强正常小鼠和免疫抑制小鼠单核巨噬细胞系统吞噬功能,恢复免疫抑制小鼠迟发型超敏反应,促进溶血素的生成。[结论]沙枣多糖可对抗Cy所致的免疫抑制,明显增强小鼠免疫功能。     

Effect of Spirulina fusiformis on cyclophosphamide and mitomycin-C induced genotoxicity and oxidative stress in mice

Spirulina fusiformis was tested for its possible in vivo protective effects against cyclophosphamide (CP) and mitomycin-C (MMC) induced genotoxicity and oxidative stress in mice. Pre-treatment with S. fusiformis (250, 500 and 1000 mg kg(-1), p.o., daily for 5 days) significantly reduced the chromosomal damage and lipid peroxidation with concomitant changes in antioxidants and detoxification systems. All the three tested doses were effective in exerting a protective effect against CP and MMC.     

乌贼墨多糖对环磷酰胺介导小鼠睾丸氧化应激损伤的干预效应

[目的]探讨乌贼墨多糖对环磷酰胺致小鼠睾丸氧化应激损伤的保护作用.[方法]随机将70只成年雄性昆明小鼠分成7组：正常组、模型组、治疗组1、治疗组2、治疗组3、治疗组4和治疗组5,通过对小鼠腹腔注射环磷酰胺进行造模,灌胃乌贼墨多糖作为化疗保护剂,检测小鼠的体重、睾丸和附睾重量、睾丸指数和附睾指数、精子畸形率、睾丸中抗氧化指标,同时观察睾丸组织结构.[结果]乌贼墨多糖能提高小鼠体重和睾丸重量,降低精子畸形率,提高睾丸组织中抗氧化酶SOD和CAT活性,降低MDA含量,修复睾丸病理学损伤.乌贼墨多糖明显缓解环磷酰胺对睾丸的氧化损伤,其最佳灌胃剂量为80 mg/kg.[结论]乌贼墨多糖有可能作为临床上的化疗辅助药物.     

中药“增免散”对免疫抑制鸡免疫功能的影响

将300只1日龄雏鸡随机分成正常对照组、中药“增免散”组、环磷酰胺组、环磷酰胺组 左旋咪唑组、环磷酰胺 “增免散”组,6日龄用鸡新城疫LaSota疫苗免疫点眼滴鼻,14、21、28、35日龄测定鸡血液ND抗体效价、免疫器官指数、细胞免疫功能、红细胞免疫粘附功能等,探索中药“增免散”的免疫增强机理。结果表明:中药“增免散”不但能显著提高Lasota活苗接种雏鸡的特异性HI抗体水平,延长其持续时间,能促进雏鸡免疫器官的生长发育,能显著增高雏鸡外周血T淋巴细胞ANAE阳性率,并对雏鸡红细胞免疫粘附功能具有显著促进作用,而且对环磷酰胺的免疫抑制有部分拮抗作用。     

鹿茸醇提物对用环磷酰胺处理的小白鼠生理生化指标的影响

用环磷酰胺处理小白鼠 ,就鹿茸醇提物对小白鼠的影响进行实验研究 ,用以探讨鹿茸醇提物对小白鼠的作用及作用机制。结果表明 :鹿茸醇提物组 (即实验组 )小白鼠血清钙、镁、磷含量分别为 2 2 4± 0 0 3mmol/L、 1 6 5± 0 0 2mmol/L、 2 2 8± 0 0 4mmol/L ,对照组则分别为 2 1 2± 0 0 3mmol/L、 1 6 4± 0 0 3mmol/L、 2 2 9± 0 0 3mmol/L ,两组相比 ,无显著性差异 (p >0 0 5 ) ;实验组小白鼠血清中高密度脂蛋白 (HDL)、总胆固醇 (CHO)、甘油三酯(TG)含量分别为 1 4 8± 0 0 7mmol/L、 2 0 7± 0 1 0mmol/L、 0 78± 0 0 8mmol/L ,对照组则分别为 1 1 5± 0 0 9mmol/L、 2 1 3± 0 1 5mmol/L、 0 82± 0 0 5mmol/L ,与对照组相比 ,实验组HDL较高 ,有显著差异 (P <0 0 5 ) ,总胆固醇、甘油三酯较低 ,差异不显著 (P >0 0 5 ) ;实验组小白鼠血清总蛋白、白蛋白、球蛋白分别为 74 6± 0 5 6g/L、 2 5 2± 0 2 9g/L、 49 4± 0 73g/L ,对照组则分别为 6 2 1± 0 2 3g/L、 2 1 3± 0 2 1g/L、 40 8± 0 2 5g/L ,实验组总蛋白、白蛋白、球蛋白极显著高于对照组 (P <0 0 1 )实验组小白鼠体重增加 2 37± 0 1 8g/只 ,而对?     

当归补血汤对环磷酰胺所致家兔血细胞减少的影响

本文主要研究当归补血汤（DBT）对环磷酰胺（Cy）所致家兔血细胞减少的影响。将40只新西兰白兔（1．7—1．9kg）随机分为4组,Ⅰ、Ⅱ、Ⅲ组按75mg／kg体重腹腔注射环磷酰胺（Cy）,连续3d,诱发家兔血细胞减少。Ⅰ、Ⅱ组分别给予1mL／kg的当归补血汤和玉屏风口服液（YPF）,Ⅲ组为环磷酰胺对照,Ⅳ组等剂量注射生理盐水作为健康空白对照。试验期15d,观察药物对免疫抑制家兔血液学指标如白细胞计数（WBC）、红细胞计数（RBC）、平均血红蛋白含量（HGB）、血小板计数（PLT）,以及体重的影响。结果显示：YPF和DBT对Cy造模家兔的血液学指标有一定程度的恢复作用,且DBT效果优于YPF,但均未能使WBC、RBC、HGB、PLT值达到正常水平;YPF和DBT对Cy造模家兔的体重增长无明显影响。本试验证明：“当归补血汤”能改善环磷酰胺所致血细胞减少家兔的血液学指标。效果优于临床常用的经典方剂“玉屏风”,但尚不能恢复到正常水平。     

环磷酰胺血药浓度测定方法（综述）

本文综述了国外60年代以来,抗癌药物环磷酰胺在血中浓度的测定方法。对常用的气相色谱法、高效液相色谱法,质谱法等主要优缺点予以分析,并对其法典型例子予以介绍。扼要介绍了血中药物的提取方法、概述了国外的一些新的进展。