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Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
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以药用植物长春花( Catharanthus roseus ( L.) G.Don)为对象,研究不同形态外源氮供应条件下,长春花生长发育与体内氮代谢响应盐胁迫的变化特点。结果表明,无盐胁迫条件下,不同形态氮源供应对长春花生长影响不显著,但硝态氮与氨态氮混合供应的情况下,硝酸还原酶( NR)和谷氨酰胺合成酶( GS)的活性及游离氨基酸总质量分数显著高于其他氮源条件下的;在盐胁迫条件下,硝态氮和氨态氮混合供应显著增加了叶片生物量积累,同时,NR活性也显著提高。总体上,混合氮源供应可以减少盐胁迫对长春花的伤害。这种作用可能与氮代谢过程中关键酶活性的增加有关,特别是与氨代谢有关的酶有关,适量的氨离子可以节省植物代谢硝态氮的能量。 相似文献
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【目的】分析长春花Catharanthus roseus受黄龙病菌Candidatus Liberibacter asiaticus侵染后多酚氧化酶(PPO)基因的表达及活性变化。【方法】根据不同作物多酚氧化酶基因PPO序列的相似性,筛选出长春花多酚氧化酶基因Pw PPO引物,PCR扩增得Pw PPO基因片段,运用qRT-PCR和生理生化方法分析长春花嫁接黄龙病芽后,不同时间长春花叶、主茎和根中Pw PPO基因的表达量和酶活性变化。【结果】长春花受黄龙病菌侵染后,叶、主茎和根中Pw PPO基因的表达分别在嫁接后30、35和40 d上调表达4.23、12.64和33.80倍,不同组织中Pw PPO基因上调表达的时间和幅度不同。染病长春花的叶、主茎和根中PPO活性分别在嫁接后30、35和45 d为对照的2.87、2.10和1.89倍。【结论】Pw PPO基因表达量与PPO活性成正比,与长春花抵抗黄龙病菌侵染的能力密切相关。 相似文献
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Seedling crops of gazania, geranium, marigold, mimulus, pansy, verbena and vinca were grown to 60% anthesis in three greenhouse temperature regimes: (a) 26 °C day/6 ± 3 °C night (LT), with a 16.5 °C mean daily temperature (MDT), (b) 26 °C day/17 ± 4 °C night, with a 21 °C MDT, and (c) variable day/night temperatures in response to heating and cooling by a phase change material energy storage module (PCM) with an 8 ± 2 °C night and a 16 °C MDT. Crops bloomed from 2 to 17 days earlier in the 26 °C/17 °C greenhouse. The morphological characteristics of all cultivars of gazania, mimulus, pansy and verbena, geranium and vinca were similar regardless of greenhouse temperature regime. Marigold diploid and triploid cultivars and mimulus were morphologically more variable than the other bedding plant species. Crops grown in the PCM and LT greenhouses had virtually identical morphological characteristics and bloomed in about the same number of days. Plant height was not affected by DIF environments in the greenhouses. The PCM and LT greenhouses had a similar MDT, but the distribution of numbers of hours of mean daily temperatures was different. 相似文献
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利用不同稀释倍数的生活污水(污水∶清水,V/V,分别为1∶0;1∶1;1∶2;0∶1)浇灌长春花结果表明,与清水灌溉相比,污灌处理土壤NH4 -N含量由59.01mg/kg提高到64.22~65.89mg/kg,NO3--N含量由2.69mg/kg提高到3.03~4.54mg/kg,速效磷含量由32.29mg/kg提高到37.40~42.41mg/kg;增加了土壤酶活性,其中土壤过氧化氢酶、脲酶及蛋白酶活性分别比对照提高10.76%~23.32%、33.41%~103.69%和31.82%~131.82%;植物根系活性由170.25μg/g.h提高到177.88~471.69μg/g.h,叶绿素含量由1.78mg/dm2提高到2.68~2.98mg/dm2,植物干物质量由11.660g/盆提高到12.130~16.577g/盆。且以稀释1倍污水灌溉处理效果最佳。用原生生活污水直接灌溉虽降低污水肥效,造成土壤Cl-的积累,但对长春花生长及干物质积累无显著影响。 相似文献
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在银川地区引种杂交矮化长春花Catharanthus niraana APRICOT等12个品种,进行种苗培育,观察其生长发育过程中的性状表现.探求各品种在本地区环境条件和栽培管理条件下,不同品种之间品质差异.结果表明:12个品种均能适应银川地区栽培;嫩枝带叶扦插成活率较高;且温室栽培生长发育良好.植株矮化,丰满,花大,色艳. 相似文献
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在路线踏查的基础上,以长春花资源较丰富的沿海地区为典型调查区域,进一步进行样方调查,对海南归化植物长春花的资源蕴藏量、生长状况和群落特征进行了研究。结果表明,长春花广泛分布于海南沿海各市、县的荒坡及防风林下,适生海拔为0~56 m。样方蕴藏量以文昌湖心村的最高,儋州下浦村的最低。长春花种质资源表型多样性丰富,表型性状变异程度高,变异系数变幅39.97%~93.35%;植株性状Shannon-Weaver多样性指数变幅1.738~2.050。多重比较表明琼海、文昌长春花资源的所有测定指标均无显著差异。但是,除主根长外,这两地的长春花资源的其他指标均显著高于澄迈、临高地区的,且多项指标显著高于儋州地区的。各地样方中多以长春花为优势种,各地的伴生物种虽有差别但并无明显规律。 相似文献
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