Advances in endostatin research

Endostatin, a 20 kD (184 aa) C-teminal fragment of collagenⅩⅧ, is the most potent inhibitor of tumor angiogenesis described so for. Endostatin was initially isolated from a murine hemangioendothelioma cell line (EOMA). Purified recombinant murine endostatin generated in E. coli bacteria injected as unfolded suspension, inhibited the growth of a varity of metastatic and primary tumors in mice. However, its widespread application has been hampered by difficulties in the large-scale production of the antiangiogenic proteins. The limitation may be resolved by in vivo delivery and expression of the antitangiogenic gene. This review summarized the advances in endostain research in recent years including structure, the mechanism of generating, function and therapy.     

Anti-angiogenesis effects of IFN-α on cultured HUVECs and human HCC bearing nude mouse

AIM:To evaluate the anti-angiogenesis effect of interferon-α (IFN-α) on cultured human umbilical vein endothelial cells(HUVECs) and human hepatocellular carcinoma (HCC) bearing nude mouse.METHODS:Anti-proliferation test,MTT test，tube-formation test,migration test on cultured HUVECs were employed and the tumor volume and microvascular desity (MVD) of IFN-α treated human hepatoma cell line (HuH7) were meassured. RESULTS:IFN-α displayed apparent inhibitory effects on cultured HUVECs in anti-proliferation test,MTT test,tube-formation test,migration test,and the tumor diameter and MVD in IFN-α treated HuH7 inoculated nude mouse group were significantly less than those in PBS treated HuH7 inoculated nude mouse group.CONCLUSION: IFN-α inhibits tumor growth through anti-angiogenesis.