Changes in plasma neurotension level of patients with coronary heart disease and its clinical significance

AIM: To investigate the plasma neurotensin(NT) concentration in patients with coronary heart disease: acute myocardial infarction(AMI),unstable angina(UA),stable angina pectoris(SAP), old myocardial infarction (OMI), and to study the relationship between the plasma NT level and the myocardial ischemia . METHODS:The plasma NT concentration of 30 patients with AMI,32 pat ients with UA,35 patients with SAP,31 pat ients with OMI and 32 normal controls were determined by radioimmunoassay(RIA). RESULTS: The plasma NT level in patients with AMI(24 h),in patients with UA when angina attacked is significantly higher than that of healthy controls. The plasma NT level in patients with SAP, in patients with OMI is not significantly different from that of healthy controls. The NT level of patients with UA when angina attacked is significantly higher than that after 2 weeks treatment (P＜0.01). The NT level of AMI patients rose from early period and reached peak value at 24 h .It began to decline at 48h,and restored to normal level at 72h. CONCLUSION: When acute myocardial ischemia occured in patients with coronary heart disease, the plasma NT level was elevated. This suggests NT may have participated in the pathophysiological course of myocardial ischemia.     

卧位型心绞痛合并阻塞型睡眠呼吸暂停综合征80例临床分析

目的探讨卧位型心绞痛与阻塞性睡眠呼吸暂停综合征（OSAS）的关系,以及临床干预呼吸睡眠对卧位型心绞痛合并阻塞性睡眠呼吸暂停综合征（OSAS）的影响．方法将80例卧位型心绞痛合并阻塞性睡眠呼吸暂停综合征（OSAS）患者随机分为两组,研究组在给予常规药物治疗的基础上,给予呼吸睡眠干预,对照组仅给予常规药物治疗．对两组患者的症状缓解情况和心电图改善、恶化等情况进行比较分析．结果研究组患者在症状改善、血压控制满意率、夜间动态心电图检测心肌缺血发生率、心电图改变、心肌梗死发生率、心绞痛发作频率、射血分数（LVEF）、心律失常发生率等方面与对照组比较,差异均有显著性（P〈0．05）．结论临床干预呼吸睡眠可改善OSAS的预后．     

Effect of tongxinluo capsule on endothelial function in stable angina pectoris patients

AIM:To investigate clinical effect of tongxinluo capsule in treating stable angina pectoris patients,and its influence on endothelial function,superoxide dismutase (SOD) and malondialdehyde (MDA).METHODS:One hundred and twenty-four stable angina pectoris patients were divided into three groups,isosorbide treatment group (41 cases),tongxinluo capsule treatment group (40 cases),tongxinluo and isosorbide treatment group (combined treatment group,43 cases).The serum concentrations of nitric oxide (NO),endothelin-1 (ET-1),SOD and MDA were determined before and after treatment.The data in traetment groups were compared with that in normal control.RESULTS:The symptoms of 3 groups were significantly improved,and the total effective rate of tongxinluo capsule group and combined treatment groups were better than that in isororbide treatment group (85.00% and 88.37% vs 73.17%,P<0.05).Before treatment,the levels of serum NO and activity of SOD in angina patients were lower than that in control group.The serum MDA and ET-1 levels were higher than those in control.The levels of serum NO and SOD activity were increased remarkably after tongxinluo capsule or tongxinluo combined treatment.However,besides the concentration of NO increased after isosorbide treatment,the levels of serum ET-1 and MDA and SOD activity were not changed.CONCLUSIONS:The results suggest that tongxinluo capsule could effectively improve the symptoms of stable angina pectoris,and it is important for tongxinluo capsule to increase NO level and decrease ET-1 product,scavenge free radical and prevent lipid peroxidation.     

Construction of lymphocyte subtracted cDNA library of unstable angina

AIM:To construct a subtracted cDNA library of differentially expressed genes in human unstable angina lymphocytes.METHODS:Suppression subtractive hybridizations (SSH) were performed between the patients with unstable angina pectoris and stable angina pectoris. Lymphocyte RNA, the obtained forward and reverse cDNA fragments were directly inserted into T/A cloning vector and transformed into E.coli JM 109 to construct a subtractive cDNA library. The inserting fragments were screened by blue and while blot screening and bacterium liqulid PCR.RESULTS:Each subtractive cDNA library contained more than 2000 positive bacteria clones. Most of them distributed between 200-600 bp inserts.CONCLUSION:The library is efficient and lays solid foundation for screening and cloning new and specific expressed genes in unstable angina lymphocyte RNA.     

Inhibitory effect of high-dose Xuezhikang on inflammatory response induced by percutaneous coronary intervention in patients with unstable angina

AIM: To study the inhibitory effect of high-dose Xuezhikang,administered before percutaneous coronary intervention (PCI) on inflammatory response induced by PCI in patients with unstable angina (UA).METHODS: All patients with UA in class Ⅲ and ⅡB according to Braunwald classification were considered for inclusion in the present study.Finally,196 patients received Xuezhikang treatment 72 h before coronary angiography and successfully performed PCI with elevated C-reactive protein (CRP) level (>3 mg/L) were randomised to 2 groups: 1.2 g/d of Xuezhikang as group A,or 2.4 g/d of Xuezhikang as group B.The levels of CRP were measured at baseline,after 3 days of therapy (before procedure) and 48 hours after PCI.The patients were followed-up for 6 months for major adverse coronary events and left ventricular ejection fraction.RESULTS: There was no significant difference in the mean CRP level among the two randomized groups (P>0.05),however,after three days of pharmacological treatment,there was significantly reduced CRP content in group A ［(5.44±1.57） mg/L vs （4.04±1.54） mg/L,P<0.05］ and in group B ［(5.42±1.36） mg/L vs （3.60±1.14） mg/L,P<0.05］ compared with admission.Measurements performed 48 hours after the procedure revealed a marked CRP level increase in group A (up to 9.22 mg/L±5.03 mg/L) and an obvious increase in groups B (up to 4.97 mg/L±1.75 mg/L,P<0.05) compared with pre-procedure.The serum level of CRP in B group was distinctly lower than that in A group before (P<0.05) and after the procedure (P<0.05),respectively.Major adverse coronary events during the 6-month clinical follow-up occurred less in group A than that in group B ［21/104 (20.2%) vs 9/92 (9.8%); patients,P<0.05］.Follow-up echocardiography revealed lower left ventricular ejection fraction in group A than that in group B (55.41%±10.93% vs 59.30%±9.99%,P<0.05).CONCLUSION: High-dose Xuezhikang therapy,administered before PCI,has better inhibition effect than low-dose on inflammatory response induced by PCI in patients with UA.Attenuation of inflammatory response may be crucial for the reduction of coronary events following invasive coronary interventions.     

心绞痛患者氧化型低密度脂蛋白自身抗体和C-反应蛋白的检测及临床意义

目的:观察心绞痛(AP)患者血清氧化型低密度脂蛋白自身抗体(A b-OX-LDL)和C-反应蛋白(CRP)的浓度变化及其与心绞痛类型的关系。方法:将符合AP诊断的72例患者分为不稳定型心绞痛(UAP)组(n=35)和稳定型心绞痛(SAP)组(n=37),另选健康体检者为对照组(n=35)。采用酶联免疫吸附法测定血清A b-OX-LDL与CRP浓度。结果:A b-OX-LDL与CRP浓度,UAP组均显著高于SAP组和对照组(P<0.01),SAP组显著高于对照组(P<0.01)。A b-OX-LDL与CRP在UAP组呈高度正相关(r=0.713,P<0.01),SAP组也呈高度正相关(r=0.702,P<0.01),对照组无相关(r=0.303,P>0.05)。结论:血清A b-OX-LDL与CRP浓度反映了心绞痛的类型,在评价缺血性心脏病病变程度方面有重要的参考价值。     

Screening of the differentially expressed genes in lymphocytes of patients with unstable angina with suppression subtractive hybridization

AIM: To screen and identify the differentially expressed genes in lymphocytes of patients with unstable angina in order to find the molecular mechanism of unstable angina . METHODS: Suppression subtractive hybridizations (SSH) and dot blot hybridizations were performed to screen the relatively differentially expressed genes in lymphocyte RNA between the patients with unstable angina pectoris and stable angina pectoris. The obtained expressed sequence tags (ESTs) were used as probes to perform Reverse Northern blot with forward and reverse suppression products. And the positive ESTs were performed RNA slot hybridization with unstable and stable angina group. The obtained ESTs were sequenced and analyzed using BLAST (nr) at NCBI. RESULTS: Three up-regulated ESTs in the unstable angina group, and one down-regulated EST in the stable angina group were obtained. All of them are sequences of known genes. CONCLUSION: All these ESTs may be associated with the unstablization of plaque of coronary artery in patients with unstable angina.     

猴头菇药效研究

1974年起作者对猴头菇的药效进行了系统的研究.研究探明,猴头蔬具有提高税体耐缺氧能力,提高心血输出量,加速血液循环,抑制肿泪细胞生长等作用.治疗胃,肠溃疡、各种胃炎的有效率达87. 7％,治疗胃、食道癌的有效率达69. 3 ％,治疗冠心病心绞痛的有效率达76. 9％。     

Complement activation in acute coronary syndromes

AIM: To evaluate complement activation in patients with all forms of acute coronary syndromes (ACS) and to examine the relationship between the degree of complement activation and myocardial injury.METHODS: The subjects were divided into 2 groups: 110 ACS patients (group ACS) and 18 healthy persons (group control).One hundred and ten patients with ACS were divided into 3 sub-group: 51 patients with ST-segment elevated myocardial infarction (STEMI),28 patients with non-ST-segment elevated myocardial infarction (NSTEMI) and 31 patients with unstable angina (UA).Complement 3 (C₃),complement 4 (C₄),troponin T (TnT) as well as creatine kinase MB (CK-MB) were evaluated.RESULTS: Plasma C₃ and C₄ peak levels were significantly higher in patients with STEMI ［(1 525±302)mg/L and (423±123) mg/L］ and NSTEMI ［(1 516±289)mg/L and (396±68) mg/L］ than those in patients with UA ［(1 275±172)mg/L and (356±91) mg/L］ and the control subjects ［(1 072±196)mg/L and (182±73) mg/L］ (P<0.01 for all).Also,C₃ and C₄ serum levels in patients with UA were significantly higher than those in control subjects (P<0.01 for all).At one-week follow-up,plasma levels of C₃ and C₄ were significantly different among various days in patients with ACS (P<0.01).Plasma C₃ and C₄ levels in ACS showed a relationship with peak creatine kinase MB (CK-MB) and troponin T (TnT) levels (P<0.01).CONCLUSION: Plasma C₃ and C₄ levels are elevated in ACS in present study.The relationship between C₃,C₄ levels and ACS suggests that the complement activation is related to necrosis within the myocardium.