二棘血蜱叮咬中华硬蜱纯化抗原免疫接种宿主后的交叉免疫反应

选用中华硬蜱105kD柱层析纯化抗原对新西兰兔进行免疫接种,再用二棘血蜱进行叮咬,并与单纯二棘血蜱叮咬组和佐剂对照组进行对比,以探讨不同种属硬蜱之间的交叉免疫抗性。结果显示:单纯二棘血蜱叮咬组吸血量为181 3±44 3mg,而二棘血蜱叮咬由中华硬蜱105kD纯化抗原免疫接种组和佐剂对照组新西兰兔后其吸血量分别为102 1±25 3mg和168 5±40 9mg,纯化抗原免疫接种组较单纯二棘血蜱叮咬组和佐剂对照组吸血量分别下降43 7%和39 4%(P<0 01)。同时,纯化抗原免疫接种组也较单纯二棘血蜱叮咬组和佐剂对照组产卵量有显著下降。该研究结果表明中华硬蜱和二棘血蜱之间存在着交叉免疫反应。     

鲜食玉米杂交种津鲜糯105选育研究

介绍了鲜食糯玉米杂交种津鲜糯105的选育过程,研究了该品种的产量性状、品种特性和适宜种植区域。结果表明,津鲜糯105具有高产、优质、抗逆性强、生态适应性好等特点,作为鲜食玉米可在国内多数地区种植。     

Localization of amino acids in germinated rice grain: Gamma-aminobutyric acid and essential amino acids production approach

Germinated rice is popularly consumed for its high gamma-aminobutyric acid (GABA) and bioactive compounds. Supporting information on how to germinate rice with high GABA content and essential amino acids is lacking. White and colored rough rice were germinated for 0, 24, 48, 72, 96, 120 and 144 h. GABA, GABA substrates and essential amino acids were also investigated using GC-MS and MALDI-MSI. GABA was more concentrated after germination and shifted into the coleoptile. High correlation was recorded between germination time and GABA (0.79–0.83, p ≤ 0.01) and glutamic acid (0.88–0.89, p ≤ 0.01). Highest rates of GABA and glutamic production were observed within 48 h of germination in both rice varieties (26.12 and 34.28 mg/100g) and reached maximum value at 96 h (31.36 and 38.75 mg/100g). Colored rice germination showed higher GABA, GABA substrates and deficient amino acids than white rice. GABA and essential amino acids drastically increased after germination, supporting the consumption of germinated rice as a functional food.     

改土灭菌增产素PS—105在啤酒大麦上的应用效果

在江苏沿海地区啤酒大麦上施用改土灭菌增产素PS—105,使啤酒大麦的分蘖期、拨节期、抽穗期、成熟期提早3～5天,成穗率也显著提高,啤酒大麦原麦品质提高到国标二级以上。每公顷施用PS—105375kg加上1／2常规氮、磷用量,基本满足啤酒大麦各个时期对氮、磷营养的需求,达到和超过常规施肥的增产效果。施用PS—105还可活化土壤中的无机营养,可以逐步提高土壤肥力。     

玉米种质系谱——武105和原武02类群的划分与论述

通过1957-2004年近50年对全国玉米自交系衍生系的种质变化材料进行广泛的收集、积累与整理研究,笔者将武105和原武02类群划分为玉米系谱十大种群之第二类。武105为优良自交系品种,在顶交种、双交种、三交种、早熟单交种方面得到了广泛利用,并进一步育成了陕单一号、黄白一号、陕单七号等,后又衍生了武109、天4等优良改良系,组配成了陕单号、户单号主要推广骨干杂交种; 山东农业科学院通过辐射处理育成原武02后,进而衍生改良了原齐号、鲁原133、鲁原92等后续系,形成了新的衍生分枝,也得到了广泛的利用;后续又衍生成新的改良系,在江苏省苏玉系列品种中占有很大比重;另在吉林通化、福建一脉相承,得到应用。武105种质西种东移,南延北扩,丰富了我国玉米种质基因库。     

Comparison of predictions of baking volume using large deformation rheological properties

Three large deformation rheological tests, the Kieffer dough extensibility system, the D/R dough inflation system and the 2 g mixograph test, were carried out on doughs made from a large number of winter wheat lines and cultivars grown in Poland. These lines and cultivars represented a broad spread in baking performance in order to assess their suitability as predictors of baking volume. The parameters most closely associated with baking volume were strain hardening index, bubble failure strain, and mixograph bandwidth at 10 min. Simple correlations with baking volume indicate that bubble failure strain and strain hardening index give the highest correlations, whilst the use of best subsets regression, which selects the best combination of parameters, gave increased correlations with R²=0.865 for dough inflation parameters, R²=0.842 for Kieffer parameters and R²=0.760 for mixograph parameters.     

抗旱冬小麦新品种晋麦105号选育报告

为了给山西省小麦生产提供抗旱、高产、优质小麦新品种,促进小麦更新换代,实现小麦持续增产稳产。以运旱20410为母本、运旱719为父本进行杂交,通过水旱交叉生态选择、多环境多点鉴定等手段,采用系谱法成功选育出了抗旱冬小麦新品种晋麦105号。2017 — 2019年参加山西省南部中熟冬麦区旱地组品种区域试验, 2 a 13点(次)平均折合产量为4 396.28 kg/hm2,较对照品种晋麦47号增产6.7%。2018 — 2019年度参加山西省南部中熟冬麦区旱地生产试验, 7个试点平均折合产量为4 158.77 kg/hm2,比对照品种晋麦47号增产6.1%。该品种株高73.6 cm,穗长6.8 cm,成穗数480.0万穗/hm2,穗粒数31.0粒,千粒重38.5g;中感条锈病,中感叶锈病,中感白粉病。适宜在山西省南部麦区旱地、黄淮旱地麦区、陕西渭北旱塬麦区及同类生态类似区种植。     

不同品种类型番茄的离体再生体系研究

以不同类型番茄品种中疏四号和中杂105为试材,通过对番茄幼苗子叶的离体培养,研究番茄不同类型品种的离体再生体系.结果表明,诱导愈伤组织及不定芽时,常规品种中疏四号最佳分化培养基为MS+ 6-BA 2.0 mg/L+ IAA 0.2mg/L,正常不定芽分化率为33.3％;F1品种中杂105最佳分化培养基为MS+ 6-BA 1.0 mg/L+ IAA 0.3 mg/L,正常不定芽分化率为28.57％;中疏四号最佳的生根壮苗培养基为MS+ NAA 0.5 mg/L,中杂105则为MS+ IAA 0.1 mg/L.中疏四号潮霉素敏感试验确定的最佳筛选浓度为5 mg/L.     

Nitrogen fluxes during the initial stage of willows and poplars in short‐rotation coppices

Among energy crops, short‐rotation coppices (SRC) are recommended to provide renewable energy. Compared to annual crops, willows and poplars are regarded as plants with low requirements for nutrients, herbicides, pesticides, and soil maintenance. However, only little is known about N‐fertilizer effects on SRC and the few studies are even inconsistent. Therefore, we studied the effects of N on yields of willows and poplars in a field experiment. The effects of N fertilization on nitrate leaching and nitrous oxide emissions from the loamy‐sand soil were also measured. Cuttings of willows (Salix viminalis clone Inger) and poplars (Populus maximovizcii × P. nigra clone max 4) were planted on farmland in 2008. The experiment was arranged in a random block design with three levels of N fertilizer (0, 50, and 75 kg N ha^–1 y^–1). After 2 y, the trees were harvested for the first time. Fertilization did not affect the yields of willows or poplars. However, the application of 75 kg N ha^–1 y^–1 caused an average increase of N leaching in the willow and poplar plots of 25 kg N ha^–1 y^–1 and 40 kg N ha^–1 y^–1, respectively. Emissions of N₂O were increased by a maximum of only 0.2 kg N ha^–1 y^–1. Further, the N fertilizer stimulated the growth of the weed biomass in case of the willow plots by 46% and of the weed N content by 52% (r = 0.53). In conclusion, in the first 2 y, SRC could be produced in a more effective and environmentally friendly manner without mineral fertilizer.     

Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection

‘Khao Dawk Mali 105’ (KDML105), a Thai aromatic rice cultivar, has been accepted in markets as a prime jasmine rice with premium prices. It has been extensively used as a parental line to develop new cultivars for rainfed lowland areas in Thailand because of its favorable quality and fragrance. However, this cultivar is highly susceptible to brown planthopper (BPH), a phloem sap-feeding insect pest of rice. The main objective of this study was to combine KDML105 essential grain quality traits with BPH resistance from the donor cultivar, ‘Rathu Heenati’. The linkage drag between Bph3 and Wx^a alleles was successfully broken by phenotypic and marker-assisted selections. All introgression lines (ILs) developed in this study showed a broad spectrum resistance against BPH populations in Thailand and had KDML105 grain quality standards. Finally this study was revealed that the ILs can be directly developed into BPH resistant varieties or can be used as genetic resources of BPH resistance to improve rice varieties with the Wx^b allele in rice breeding programs.