全文获取类型
收费全文 | 1356篇 |
免费 | 95篇 |
国内免费 | 242篇 |
专业分类
林业 | 29篇 |
农学 | 35篇 |
基础科学 | 32篇 |
66篇 | |
综合类 | 363篇 |
农作物 | 30篇 |
水产渔业 | 16篇 |
畜牧兽医 | 1066篇 |
园艺 | 7篇 |
植物保护 | 49篇 |
出版年
2024年 | 16篇 |
2023年 | 54篇 |
2022年 | 56篇 |
2021年 | 76篇 |
2020年 | 78篇 |
2019年 | 87篇 |
2018年 | 35篇 |
2017年 | 42篇 |
2016年 | 54篇 |
2015年 | 61篇 |
2014年 | 99篇 |
2013年 | 110篇 |
2012年 | 130篇 |
2011年 | 106篇 |
2010年 | 74篇 |
2009年 | 95篇 |
2008年 | 63篇 |
2007年 | 59篇 |
2006年 | 71篇 |
2005年 | 55篇 |
2004年 | 51篇 |
2003年 | 52篇 |
2002年 | 18篇 |
2001年 | 30篇 |
2000年 | 19篇 |
1999年 | 17篇 |
1998年 | 12篇 |
1997年 | 9篇 |
1996年 | 11篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 8篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 6篇 |
1984年 | 1篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有1693条查询结果,搜索用时 15 毫秒
1.
2.
3.
Walter GL McGraw P Tvedten HW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(1):23-27
An enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2-diglyceride by lipase. The manual method was the Cherry-Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital-induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneumonia. 相似文献
4.
从动物耳皮肤组织采样 ,采用将组织块剪碎后直接贴附于培养瓶底部的方法进行原代培养 ,该方法使原代细胞出现率及可传代率均达到 10 0 %。根据上皮样细胞和成纤维样细胞贴壁紧实程度的不同 ,用 0 .0 5 %的胰蛋白酶-EDTA对其进行消化 ,可将两种不同类型的细胞进行分离和纯化。通过脂质体介导 ,以BLG -hINS(含乳球蛋白调控基因的人胰岛素原基因 )基因作为目的基因、GFP(绿色荧光蛋白 )基因作为标记基因共转染绵羊成纤维细胞 ,经G - 4 18筛选后 ,得到转染细胞。对转染的细胞分别用单细胞显微操作法和有限稀释法进行细胞克隆 ,两种方法均可得到克隆细胞。选形态正常、生长均匀的 5个细胞克隆进行PCR检测 ,结果 5个克隆均转有GFP基因 ,其中两个转有BLG -hINS基因。高代培养细胞、转染细胞和克隆细胞经核型分析后 ,染色体数目均为 2 7对 ,表明绵羊耳的成纤维细胞建立细胞株后 ,可以作为外源基因转染的有效供体细胞。 相似文献
5.
6.
Network on veterinary medicines initiated by the European Federation For Pharmaceutical Sciences
下载免费PDF全文
![点击此处可从《Journal of veterinary pharmacology and therapeutics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
L. Dencker R. T. Cristina H. Linden I. Schmerold 《Journal of veterinary pharmacology and therapeutics》2018,41(3):378-383
The European Federation for Pharmaceutical Sciences (EUFEPS) was founded 25 years ago by more than 20 national pharmaceutical societies and faculty members. As a pan‐European organization, it brings together pharmaceutical societies as well as academic, industrial and regulatory scientists engaged in drug research and development, drug regulation and education of professionals working in these fields. EUFEPS represents pharmaceutical sciences in Europe and is recognized as such by both the European Commission and the European Medicines Agency. EUFEPS cooperates with the European Federation of Pharmaceutical Industries and other European organizations and maintains global connections with agencies such as the US Food and Drug Administration and the American Association of Pharmaceutical Scientists. EUFEPS has established specified networks forming the basis of its activities. The creation of a Network on Veterinary Medicines is prompted by the manifold problems resulting from the use of veterinary drugs and its inherent interconnections with human medicine, environmental and public health. A long‐term goal of this initiative was to expand the spectrum of available therapeutics for use in animals, including the development of innovative delivery systems. 相似文献
7.
Acupuncture and traditional Chinese veterinary medicine for treatment of a mare with rectal paralysis
下载免费PDF全文
![点击此处可从《Equine Veterinary Education》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M. M. Sleeper K. Fishman R. J. MacKay M. Brown A. G. Bauck H. Xie 《Equine Veterinary Education》2018,30(9):460-463
An 8‐year‐old Appaloosa mare with rectal paralysis due to a cosmetic ethanol ‘tail block’ was treated with traditional Chinese veterinary medicine treatments including acupuncture and herbal medicine. Her rectal and tail tone gradually improved after the treatment. At 4 months after initial presentation, the mare was able to produce faecal piles on a regular basis, and manual evacuations were no longer needed. Significant improvement was within 30 days of beginning treatment. At 8 months, the owner indicated that the mare had normal defaecation, was able to swish the tail from side to side and lift the tail to urinate, and had no evidence of straining to defaecate or colic. 相似文献
8.
A potentially significant cause of damage to grassland soils is compaction of unsaturated soil and poaching of saturated or nearly saturated soil by animal hooves. Damage is caused when an applied stress is in excess of the bearing strength of the soil and results in a loss of soil structure, macroporosity and air or water conductivity. Severely damaged soils can cause reduced grassland productivity and make grazing management very difficult for the farmer. The actual amount of soil damage that can occur during grazing is dependent on the grass cover which acts as a protecting layer, the soil water content and the characteristics of the grazing animal (weight and hoof size). Assuming that the farmer is knowledgeable about the characteristics of the grazing animal and grass cover, it would be very useful for short‐term operational farm planning to be able to predict when soil water contents were likely to be in a critical range with respect to potential hoof damage. In this study soil moisture deficits (SMDs) which can be derived from meteorological forecasts are evaluated for predicting when soil water conditions are likely to lead to hoof damage. Two contrasting Irish grassland soils were analysed using a Hounsfield servo‐mechanical vertical testing machine to simulate static (285.4 N) and dynamic (571 N) hoof loads on the soil over a range of estimated SMDs (0, 5, 10 and 20 mm). The deficits were analysed with respect to the soil volumetric water content, compression (displacement) and change in dry bulk density. The SMDs imposed in the laboratory were similar to those under field conditions and thus the methods used in this study are applicable elsewhere. The change in dry bulk density following loading (0.2–0.7 g/cm3) was linearly related to SMD (R2 ranged from 0.90 to 0.99), leading to the conclusion that a forecast of SMD can be used to predict when grassland soils are likely to be at risk of damage from grazing. 相似文献
9.
10.