Expression and modulation of connective tissue growth factor in renal interstitial fibrosis

CTGF, a member of the CCN family of immediate early genes, is a recently discovered profibrotic growth factor, which is involved in many pathophysiologic procedures. CTGF acts as a downstream effector of TGF-β acting on interstitial cells to enhance the progression of fibrotic renal diseases. It has been shown that CTGF gene expression can be induced or blocked by some kinds of cytokine and drugs. It is an interesting candidate target for future intervention strategies of renal interstitial fibrosis.     

贮藏中福桔果皮细胞结构的显微观察及果实理化成分测定

贮藏期间福桔果皮细胞始终具有分裂增殖能力,细胞生长的养分主要通过维管束向果肉吸取,因而促进果肉衰老,形成枯水.枯水果实在贮藏早期就发现果皮细胞层和油腔分泌细胞无丝分裂旺盛,双核细胞多,果皮增厚多.严重枯水时,果皮细胞还具细胞核、线粒体、有色体等超微结构,未枯水果实细胞分裂少见.经预贮后贮藏的果实,由于中断了果皮组织与维管束的联系,果皮细胞生长受抑制,果肉水分、养分消耗少,枯水率最低,     

Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuang long-Jie gu Pill (SLJGP) containing serum osteoblast proliferation

AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A.     

猪胸膜肺炎放线杆菌外膜蛋白(OMP)5′末端保守区基因的克隆、表达及其免疫活性研究

以猪胸膜肺炎放线杆菌血清7型25-4株基因组DNA为模板,PCR方法扩增外膜蛋白(OMP)5′末端保守区基因片段(OMPc),酶切及核苷酸序列分析鉴定后,与原核表达载体质粒pGEX-6P-1进行连接,构建成重组表达载体pGEX-OM-Pc,转入大肠杆菌BL21中,以IPTG进行诱导,SDS-PAGE电泳分析发现,转化了重组质粒的菌株所表达的融合蛋白相对分子量为34 kD,与实际预测相符,命名为GST-OMPc.GST亲和层析柱进行纯化,ELISA方法对纯化蛋白进行检测.结果表明:纯化蛋白GST-OMPc能够与兔抗猪胸膜肺炎放线杆菌血清7型的阳性血清反应.OMPc蛋白的成功表达为其功能的研究打下基础.     

法氏囊活性肽BP7调节鸡未成熟B细胞的基因表达谱及功能分析

试验旨在探索法氏囊活性肽BP7调节鸡未成熟B细胞的分子基础.利用BP7刺激禽前B淋巴细胞DT40细胞,采用荧光定量PCR(qPCR)检测IgM的mRNA水平,并采用基因芯片分析基因表达谱及其生物学功能.结果 显示,BP7刺激的DT40细胞产生IgM的mRNA水平明显升高.基因芯片分析发现,BP7处理的DT40细胞中共有...     

茶树LOX基因家族的鉴定及其在白茶萎凋过程的表达分析

脂肪族类化合物是植物芳香物质的重要组成部分,对白茶香气的形成具有重要作用.利用生物信息学方法,在染色体级别的茶树基因组数据库中,对LOX基因家族进行鉴定,从中获得12个茶树LOX基因家族成员,命名为CsLOX1～CsLOX12.12个茶树LOX基因序列,主要定位于细胞质或叶绿体中,其编码蛋白具有相同的特征结构域及保守基...     

SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

• A total of 8 SOD genes from watermelon were identified and bioinformatically analyzed. • The SOD proteins from watermelon and other different plant species can be classified into five groups consistent with their metal cofactors. • ClSOD genes exhibited distinctive tissue-specific and abiotic stress responsive expression patterns. Superoxide dismutase (SOD) is an important enzyme in the antioxidant system of plants and plays a vital role in stress responses by maintaining the dynamic balance of reactive oxygen species (ROS) concentrations. Genome-wide analysis of the SOD gene family in various plant species has been conducted but little is known about this gene family in watermelon (Citrullus lanatus). Here, eight SOD genes were identified in the watermelon genome and are designated ClCSD1-5, ClFSD1-2 and ClMSD according to their metal cofactors. Phylogenetic analysis shows that SOD proteins from various plant species can be classified into five groups and members in the same group possess the same metal cofactor and similar subcellular localizations. Expression analysis of the ClSOD genes indicates that they had tissue-specific expression patterns with high expression in different tissues including the leaves, flowers and fruit. In addition, the expression of ClSOD genes differed appreciably under salinity, drought and abscisic acid (ABA) treatments, indicating that they may be involved in ROS scavenging under different abiotic stresses via an ABA-dependent signaling pathway. These results lay the foundation for elucidating the function of ClSOD genes in stress tolerance and fruit development in watermelon.     

携带猪流行性腹泻病毒S1抗原表位的乙肝核心抗原病毒样颗粒的构建与鉴定

旨在构建以乙肝核心抗原(HBcAg)为载体呈现猪流行性腹泻病毒(PEDV)S1抗原表位的病毒样颗粒.将PEDV S1蛋白中含B细胞表位的270 bp片段插入到HBcAg主要免疫显性区域(MIR),构建重组质粒pET-32a(+)-HBcAg-PEDV S1,转化到感受态细胞BL21(DE3)中,经IPTG诱导表达,SD...     

微塑料对双酚A在鲤鱼肠道中累积及生长生理的影响

探明微塑料（MPs）对双酚A （BPA）在鱼类肠道中累积特征及鱼类生长生理的影响,本研究以黄河鲤鱼为模型生物,分析了0.5 μm聚苯乙烯微塑料（PS-MPs）与BPA联合作用下鲤鱼肠道中微塑料及BPA的累积特征、鲤鱼生长及其抗氧化酶活性,以及它们之间的响应关系。结果表明：联合处理下鲤鱼肠道中的PS-MPs含量随时间呈线性增加,且随投加的微塑料浓度增加而增加,而PS-MPs累积速率随时间呈先增加后减小趋势,其中在BPA+PS（L）处理（1 mg·L^-1 BPA+20 μg·L^-1 PS-MPs）下,0.25 d时鲤鱼肠道中PS-MPs累积速率达到峰值（373.81 μg·g^-1·d^-1）,BPA+PS（H）处理（1 mg·L^-1 BPA+100 μg·L^-1 PS-MPs）下,则是在0.125 d达到最高（644.00 μg·g^-1·d^-1）。与单一BPA暴露相比,联合暴露下鲤鱼肠道中BPA累积量增加,与BPA （1 mg·L^-1 BPA）处理相比,BPA+PS （L）和BPA+PS （H）处理下鲤鱼肠道中BPA含量分别提高了6.13%和9.74%,BPA累积速率分别增加了190.01%、373.80%。相较于对照处理,BPA处理和MPs与BPA联合处理均引起鲤鱼肠组织中超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性、还原型谷胱甘肽（GSH）含量、丙二醛（MDA）含量增加,使体质量下降幅度减缓,其中BPA、BPA+PS （L）和BPA+PS （H）处理下鲤鱼体质量（21 d）较对照处理分别下降1.22%、3.90%、4.33%。当微塑料投加量达到100 μg·mL^-1时,联合处理会增加鲤鱼肠道中SOD活性、CAT活性和GSH含量,抑制MDA产生,从而缓解MPs和BPA联合作用对鲤鱼造成的氧化损伤,并且减缓鲤鱼体质量下降。研究表明,微塑料的存在会增加双酚A在鲤鱼肠道中的累积,且两者联合作用对鲤鱼产生了协同毒理效应,导致鲤鱼生长速率减缓,体质量下降。