模糊评分法在高羊茅品种综合评价中的应用

1999--2001年对引进和培育的高羊茅新品种(系)在喀斯特生态区进行品种比较试验。观测记裁各参试品种的性状表现，对春、夏、秋、冬4个季节的观测结果，用模糊评分法Ⅲ进行分析比较，并按综合评价值的大小排序a结果高羊茅“98--1”的综合评价值最大，排第一名，依次是猎狗5号、矮星2号、凌志(对照)，其余参试品种排名次后。认为用模糊评分法对高羊茅品种进行分析评价是可行的。     

矮壮素对高羊茅生长特性影响的研究

试验采用0.8,1.0,1.2 mg/g 3种浓度的矮壮素(CCC)对草坪草高羊茅Festuca arundinacea进行处理,结果表明:3种浓度的矮壮素对高羊茅生长均产生不同程度的影响,其中 0.8 mg/g矮壮素处理使高羊茅株高、修剪量明显降低.综合考虑药效及成本,建议在实际生产中应用0.8 mg/g的矮壮素浓度.     

24份加拿大牧草品种引种试验

1986年我们从加拿大碧青种子公司引进牧草种子24份,其中豆科7份,禾本科17份,于当年秋播。从3年试种结果看,Glory苜蓿、Minto苜蓿、Algonquin苜蓿在北京种植,无论是产草量、种子产量、草质还是适应性均表现良好。Leo百脉根在北京可安全越冬,其特点是夏季生长繁茂,草质好,耐牧,耐践踏。Orbit长穗冰草、Chief 中间冰草、阿尔泰野麦草表现为抗寒耐旱,产草量高,但要适时利用,否则会降低饲用价值。俄罗斯野麦草、Nordan沙生冰草、草地看麦娘、猫尾草产草量一般,但草质好,春季返青早,枯黄期晚,可在京郊西部山区种植。无芒雀麦(2份)、鸭茅、牛尾草、(艹鹝)草(3份)均表现为适应性好,草质优,产草量高,是较好的禾本科牧草。白三叶、草地早熟禾可做为地被植物和草坪草。     

Detection, identification and significance of phytoplasmas in grasses in northern Australia

Sugarcane yields have been severely reduced by white leaf and grassy shoot phytoplasma diseases in many parts of Asia. Australian sugarcane crops are not known to be affected by these diseases, but plant pathogenic phytoplasmas found in other introduced and native grasses in northern Australia could pose a serious threat to the Australian sugarcane industry. To further evaluate this threat, leaves from plants of 20 grass species, with and without symptoms, were collected during field surveys in northern Australia and tested to determine whether phytoplasmas were present and whether symptoms were reliable indicators of phytoplasma presence. Molecular tools were used to detect and characterize phytoplasmas. Four different phytoplasmas were found in seven grass species known to grow near healthy sugarcane crops. All the phytoplasmas were closely related to sugarcane white leaf phytoplasma (SCWL), one of the phytoplasmas that causes disease in sugarcane in Asia. Four of the host plant species and two of the phytoplasmas were new records. The relationship between symptoms and phytoplasma presence was poor. Because some plants with symptoms tested negative for phytoplasmas, a series of surveys was carried out in which flowers, leaves, roots and stems of two known host plant species, Whiteochloa cymbiformis and Sorghum stipoideum, were tested separately on nine occasions during two wet seasons. This was done to investigate the distribution of phytoplasmas within plants over time. Results showed that spatial and temporal variation of phytoplasmas occurred in these two host plant species. Hence, evaluation of disease distribution within a region requires repeated testing of all plant parts from plants without symptoms, as well as those with symptoms. To date, there is no report of a vector capable of transmitting to Australian sugarcane the phytoplasmas found in grasses in this study. If one is present, or occurs in the future, then native and introduced grasses could constitute a large reservoir of phytoplasma for vectors to draw on. This work provides an early warning for the sugarcane industry that the potential for infection exists.     

Tall oil fatty acid inclusion in the diet improves performance and increases ileal density of lactobacilli in broiler chickens

1. Studies were conducted with tall oil fatty acids (TOFA) to determine their effect on broiler chicken performance and ileal microbiota. TOFA, a product originating from coniferous trees and recovered by fractional distillation of side-streams from pulp production, mainly comprises free long-chain fatty acids (~90%) and resin acids (~8%). Conjugated linolenic acids and pinolenic acid are characteristic fatty acid components of TOFA.

2. TOFA products at 750 mg/kg feed were tested in two 35-day broiler chicken trials, each using a wheat soya-based diet and with 12 replicate pens per treatment. In both trials, TOFA improved body weight gain at all time points (P < 0.001) and feed conversion efficiency during the first 21 days (P < 0.01). Two different dry TOFA formulations (silica carrier and palm oil coating) were tested and showed performance effects similar to liquid TOFA.

3. Ileal digesta of the broiler chickens was analysed for total eubacteria, Lactobacillus spp., Enterococcus spp., Escherichia coli and Clostridium perfringens on days 14 and 35. TOFA significantly increased total eubacteria and lactobacilli density on day 14 (P < 0.05). There was a significant positive correlation between these bacterial groups and broiler body weight on day 14 (P < 0.01).

4. A numerical reduction in C. perfringens was observed. In vitro growth inhibition studies showed that C. perfringens was strongly inhibited by 10 mg/l TOFA (P < 0.001), while common lactobacilli were resistant to >250 mg/l. The in vitro results were thus in line with in vivo observations.

5. The mechanisms behind the bacterial shifts and their role in performance improvement are unknown. Further purification of TOFA components is needed to identify the effective agents.     

Environmental performances of giant reed (Arundo donax L.) cultivated in fertile and marginal lands: A case study in the Mediterranean

Perennial rhizomatous grasses (PRGs) tend to have a high yield combined with a low environmental impact. Cultivation in marginal or poorly cultivated land is recommended in order not to compromise food security and to overcome land use controversies. However, the environmental impacts of using different types of soil are still unclear. We thus assessed the environmental impact of two giant reed (GR) systems cultivated in a fertile soil (FS) and in a marginal soil (MS) through a cradle-to-plant gate LCA. We analyzed energy balance, GHG emissions (including LUC, not including iLUC), and the main impacts on air, water and soil quality. In both systems the annualized soil carbon sequestration was more than twofold the total GHG emitted, equal to −6464 kg CO₂eq ha⁻¹ in FS and −5757 kg CO₂eq ha⁻¹ in MS. Overall, soil characteristics affected not only GR yield level, but also its environmental impact, which seems to be higher in the MS system both on a hectare and tonne basis. The production of GR biomass in marginal soil could thus lead to higher environmental impacts and a more extensive land requirement.     

Unravelling the relationship between a seasonal environment and the dynamics of forage growth in grazed swards

It is well reported in the scientific literature that pastures can have similar net forage accumulation when managed with contrasting structures. However, we hypothesized that the dynamics of forage accumulation in grazed swards is linked to seasonal-environmental conditions. Marandu palisadegrass (Brachiaria brizantha [Hochst. ex A. Rich.] was used as the forage species model. The experimental treatments were four grazing heights (10, 20, 30 and 40 cm) allocated to experimental units according to a randomized complete block design with four replicates and evaluated throughout four contrasting environmental seasons (summer, autumn, winter–early spring and late spring). Under rainy and warm periods, greater net forage accumulation was observed in pastures maintained taller; on the contrary, during the mild and dry periods, net forage accumulation rate reduced as grazing height increased. Such patterns of responses were related to compensations between tiller population density and tissues flows during summer and late spring and the reduced capacity of taller canopies to compensate lower population with greater growth rates during autumn and winter–early spring. Grazed swards changed their patterns of forage growth as they transitioned from favourable to more abiotic stressful conditions, suggesting that seasonal adjustments in grazing intensities are necessary in order to maximize forage production.     

Field Evaluation of Cocksfoot,Tall Fescue and Phalaris for Dry Marginal Environments of South‐Eastern Australia. 1. Establishment and Herbage Production

Newly developed candidate cultivars of cocksfoot (Dactylis glomerata L.), phalaris (Phalaris aquatica L.) and tall fescue (Festuca arundinacea Schreb. = syn. L. arundinaceum (Schreb) Darbysh.) were evaluated over four years for persistence and productivity against current commercial cultivars in small plots at five locations selected for lower and less reliable rainfall and difficult soils (low pH and high Al) in south‐eastern Australia known to be marginally too dry for these grass species. The five locations were ‐ representing summer dominant rainfall, Inverell, in northern New South Wales (NSW); ‐ representing uniform rainfall; Trungley Hall, (medium rainfall), and Beckom (lower rainfall) both in southern NSW; and; representing a winter dominant pattern ‐ Eversley, (higher rainfall), and Bealiba, (lower rainfall), in central Victoria. The objective was to determine if the new candidate cultivars were more likely to persist and to be productive than current commercial cultivars. The study showed that most phalaris and cocksfoot treatments were highly productive in high rainfall years at one or both sites in southern NSW. However, all treatments had become much less productive by the end of the experimental period due to plant loss under hot, dry conditions in the final summer. At Bealiba in central Victoria, cocksfoot was the most productive species with several cocksfoot treatments of both subsp. hispanica and subsp. glomerata still present at the final harvest despite a hot and dry final summer‐autumn. Tall fescue was the most productive species in the two higher rainfall environments (Inverell, Eversley) although most treatments of all species performed well at those sites. Continental tall fescues were more productive on average than Mediterranean tall fescues at the strongly acidic Eversley site. As a result of this work, two of the new cocksfoot candidates (Moroccan Fine and AVH48 Selection) and one of the tall fescue candidates (Summer Active 1) have been licensed for commercial development and release.     

Mild Drought Stress‐Induced Changes in Yield,Physiological Processes and Chemical Composition in Festuca,Lolium and Festulolium

The impact of mild drought stress (3 weeks at 40 % field water capacity) on yield, physiological processes, accumulation of proline and phenolic compounds and forage quality parameters in forage grasses was evaluated in pot experiments. During four different growing periods, the effects of water deficit were assessed in nine varieties from five species (Lolium perenne, Lolium multiflorum, Festuca pratensis, Festuca arundinacea and Festulolium braunii). All measured parameters were affected by drought stress in the different cuts. Photosynthesis, transpiration rate, stomatal conductance and dry matter yield were significantly lower in drought stress than under well‐watered conditions in all varieties. Higher water‐use efficiency was only observed during the first and fourth drought period, while this was not the case in the second and third. Mild drought stress significantly increased the content of proline, phenolic acid, flavonoids, water‐soluble carbohydrates and protein. All tested grasses showed also an increase of organic matter digestibility and cell wall digestibility under drought stress conditions.     

草地早熟禾与高羊茅不同混播比例对草坪质量的影响

在大田条件下,研究了草地早熟禾和高羊茅不同混播比例对草坪质量的影响,以期为北方地区草坪建植提供依据。结果表明:播量一定时,随草地早熟禾比例减小、高羊茅比例增大,草坪成坪速度越快,密度越小,质地越差,颜色越深,地下植物量越大,地上植物量呈先增大后减小的趋势,草地早熟禾∶高羊茅为2∶8处理组合地上、地下植物量均最大。当高羊茅在混播组合中的比例60%后,草坪密度、质地、地上植物量趋于稳定。6种处理组合草坪综合质量从优到劣依次为AFEBCD。综合考虑成坪速度和草坪质量,草地早熟禾和高羊茅混播建坪的比例以2∶8为宜。