Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.     

Comparative effect of advanced soy products or corn protein concentrate with porcine meal on growth,body composition,and distal intestine histology of Florida pompano,Trachinotus carolinus

The present study was designed to investigate the effects of diets containing advanced soy products (enzyme‐treated soy and fermented soy) or corn protein concentrate (CPC) in combination with porcine meal (PM) to completely replace poultry byproduct meal (PBM) on growth performance, body composition, and distal intestine histology of Florida pompano, Trachinotus carolinus. Four experimental diets were formulated to be isonitrogenous and isolipidic, to contain 400 g/kg crude protein and 80 g/kg lipid. A reference diet (PBM diet [PBMD]) contained 150 g/kg PBM and 495 g/kg soybean meal (SBM), and three test diets were formulated replacing PBM with 15 g/kg of CPC (CPC diet [CPCD]) or replacing all SBM and PBM with 535 g/kg fermented soy (fermented soybean meal diet [FSBMD]) or 451.3 g/kg enzyme‐treated soy (enzyme‐treated soybean meal diet [ESBMD]). All three test diets were supplemented with 38 g/kg of PM. Diets were fed based on a percentage of bodyweight adjusted after sampling the fish every 2 weeks to triplicate groups of Florida pompano juveniles (mean weight 8.06 ± 0.22 g). After 8 weeks of feeding, fish fed CPCD and ESBMD performed equally well in terms of final body weight, thermal growth coefficient, and percentage weight gain in comparison to fish fed PBMD. In all cases, feeding FSBMD resulted in poor feed conversion and lower feed intake compared to other treatments. Protein retention efficiency, whole‐body proximate composition, phosphorus, sulfur, potassium, magnesium, calcium, sodium, and zinc contents were not significantly influenced by the dietary treatments. The results obtained in the present histological study showed no significant differences in the thickness of serous layer, muscular layer, and submucosal layer of the intestine among treatments. Fish fed CPCD showed a significant widening of the lamina propria with an increase of cellular infiltration and higher presence of goblet cells compared to other dietary treatment. Based on these results, 451 g/kg ESBM or combination of 150 g/kg of CPC and 495 g/kg SBM supplemented with 38 g/kg PM can be utilized to develop a practical diet for juvenile Florida pompano without impacting growth, nutritive parameters, and several distal intestine health parameters.     

以塘坝为中心建设立体型综合开发小区

以塘坝为中心建设立体型综合开发小区于明（黑龙江省青冈县水土保持办公室，１５１６０６）塘坝是我国水利水保建设的重要工程措施之一。它不仅可防洪蓄水，防治水土流失，同时对解决农田抗旱灌溉、养鱼等综合开发利用具有重要作用。搞好以塘坝建设为中心的小流域综合开发...     

The Application of GIS in Small Watershed Classification in Loess Plateau

ＴｈｅＡｐｐｌｉｃａｔｉｏｎｏｆＧＩｓｉｎＳｍａｌｌＷａｔｅｒｓｈｅｄＣｌａｓｓｉｆｉｃａｔｉｏｎｉｎＬｏｅｓｓＰｌａｔｅａｕＺｈｕＪｉｎｚｈａｏ，ＷｕＢｉｎ，ＢｉＨｕａｘｉｎｇ，ＺｈｏｕＣｈａｎｇｑｉｎｇＣｏｌｌｅｇｅｏｆＳｏｉｌａｎｄＷａｔｅｒＣ...     

Histopathology of Experimental Schistosoma bovis Infection in Goats

The inflammatory host response to Schistosoma bovis in young goats was studied at necropsy by light microscopy 34 weeks after primary exposure to 3,000 cercariae (group B, n=6), 34 weeks after primary exposure to 3,000 cercariae followed by challenge with 2,500 cercariae at week 17 (group C, n=5), and 17 weeks after primary exposure to 2,500 cercariae, given on week 17 of the experiment (group D, n=6). Three goats served as uninfected controls. The faecal egg output had been minimal for 17 weeks prior to necropsy in groups B and C and only for the last 2 weeks in group D.Histological studies were carried out on the small intestine, liver, lung and spleen, and tissue egg counts were performed. In sections of the small intestine and liver, a panel of histopathological variables were quantitated to characterize the host response and differences between groups of animals were evaluated with one way analysis of variance. The mean tissue egg count in the small intestine was slightly but not significantly higher in group C than group B and about twice as high in group D (D vs B or C p<0.01). Group means of numbers of inflammatory foci per section of gut wall corresponded well with those of tissue egg counts, suggesting that the rate of inflammatory destruction of eggs did not differ markedly between the groups. Egg material was less commonly seen in granulomas of the small intestine in group B than in group D (p<0.01), suggesting lower passage of eggs through the gut wall during the later than during the earlier phase of patent primary infection. The frequency of eosinophil-rich hepatic inflammatory foci was much higher in group D than in the other groups (D vs B p<0.05, D vs C p< 0.01), and coincided with a high degree of blood eosinophilia in this group at the time of sacrifice. Challenged goats showed a significantly higher frequency of markedly fibrotic inflammatory foci in the liver and of liver granulomas with a marked giant cell component than goats of the other groups. Hepatic portal fibrosis was least prominent in animals with 17- week- old primary infections, implying a possible relation between this change and duration of infection.     

新疆水土保持情况调查

新疆水土保持情况调查郭廷辅，佟伟力（水利部水土保持司，北京１００７６１）我们于１９９４年８月１１日至２６日对新疆伊犁、和田、喀什、吐鲁番、昌吉、乌鲁木齐等６个地区（州、市）的１１个县（市）、两个流域的水土流失情况和开展治理工作做了典型调查。同时，也对...     

杂交条纹鲈苗种小水泥池培育技术试验

用地上露天小水泥池8个．进行杂交条纹鲈苗种培育试验．放养313龄水花30000尾．通过天然饵料、藻粉投喂以及适当水质管理措施．经32天培育，共培育3．5～4．6cm体长的鱼种4336尾．最高成活率为19．1％，最低为6％，平均为14．5％。     

人工培育牛黄对小白鼠离体小肠运动的解痉作用

本文证明培育牛黄对乙酰胆硷与氯化钡所引起的小白鼠离体小肠痉挛有解痉作用。培育牛黄的成份中去氧胆酸具有较强的抗乙酰胆硷与氯化钡作用,其浓度与效应呈直线相关。其成分中的胆酸仅有较弱的抗乙酰胆硷作用,且浓度与效应不呈直线相关,也无抗氯化钡作用。     

小流域治理成果的开发利用途径

根据市场经济的要求和目前小流域治理中普遍存在的高投入、低效益问题，提出以市场经济为导向，通过合理开发利用治理成果，优化组合资源、资金、劳力、技术等生产要素，努力发展商品生产，提高小流域治理的经济效益。结合各地实践，总结出基本农田的集约经营、水保林的经营增值、水保草的养畜转化、非生产地的开发利用、转化产品的加工增值和疏通流通渠道六条开发利用途径。     

Small intestine and small colon neuropathy in equine dysautonomia (grass sickness)

The number of neurons in the coeliacomesenteric ganglia and the myenteric and submucosal plexuses of the jejunum, ileum and small colon, and the pathological changes induced in them, were studied in various types of equine dysautonomia. In all forms of dysautonomia, severe and extensive neuron loss and damage occurred in the ileum. In acute and subacute dysautonomia, jejunal neuron loss and damage were severe, but in chronic cases significantly less loss or damage occurred. The damage followed the same pattern in the small colon but it was always less obvious than in the jejunum. The distribution of the damage was uniform within a segment of the intestine. In fatal cases of dysautonomia, the clinical severity and duration of illness seems, in most instances, to be related to the amount of neuronal disruption occurring in the jejunum. Severe disruption results in acute/subacute dysautonomia, while milder damage leads to the chronic form.No case of dysautonomia was encountered in which enteric neuron loss and damage occurred without significant neuronal disruption also occurring in the coeliacomesenteric ganglia.Ileal neuronal damage and loss are not invariably worse than that in the jejunum, and the possible reasons for this, together with the relationship between neuronal damage and possible causes of dysautonomia, are discussed.Abbreviations H&E haematoxylin and eosin Deceased. Formerly of the Moredun Research Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH, UK