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1.
对6头试验公鹿用假台鹿采精,采精前对特制的假阴道以药用凡士林为滑润剂,注水充气,温度为40℃±1℃,以适宜的压力和滑润度,将其安装在人工制做的假台鹿内。拨赶公鹿于采精场诱其爬跨。结果,试验的6头公鹿中有4头能够采精,共22头次,射精量平均每头次1.54ml,精子密度为18.6—37亿/ml,精子活力在0.8以上。93年每头次生产细管冻精106.25支(425支/4头次),是同年电刺激采精法平均64.4支/头次(902支/14头次)的1.65倍,此法既能采到质量高的精液,又比电采法省工、省时、省力、安全,是一种理想的马鹿采精方法。 相似文献
2.
R. Thomassen 《Reproduction in domestic animals》1993,28(4):289-293
Contents: An insemination trial using frozen semen is described. The freezing procedure was slightly modified from the Hannover method. The insemination dose consisted of 7 medium straws containing approx 1 109 spermatozoa. A total of 28 mares of the Norwegian Trotter breed were inseminated during the 1991 season. During oestrus the mares were examined at 12 hour intervals, and the insemination was carried out after detection of ovulation. The pregnancy rate was 43% after the first insemination, increased to 68% after second and further to 75% after the third and last insemination. The foaling rate was 61.5%. 相似文献
3.
For practical reasons, a large volume (i.e. 5 ml) of frozen boar semen per insemination dose is desirable, but successful freezing has not been achieved, since optimal cooling rates have not yet been established. Post-thaw motility and the acrosome intep'ty of semen from four boars frozen with a programmable freezin machine, in mini-(0.25 ml), maxi-(5 ml) plastic straws and in 10 × 5 cm PVC- or Teflon FEP-plastic bags (0.35 – 0.12 mm thick, 5 ml) was studied. The freezing of the semen was monitored using thermocouples placed in the straws and the bags. The freezing curve started from +5°C, at a rate of −3°C/min, to – 6°C, it was held for 1 min at −6°C, and was followed by further drop to −100°C at a rate of −20°C/min, with subsequent storage in LN2 . The bags had a much shorter freezing point plnteau, compared to the maxi-straws. Post-thaw sperm motility was significantly higher when semen was frozen in mini-straws or in bags than in maxi-straws. The freezing procedure did not cause major acrosomal damages, significantly more normal apical ridges being present in the bags and mini-straws than in the maxi-straws. This in vitro evaluation indicates that the freezing method employed is satisfactory for freezing large volumes of boar semen into plastic bags . 相似文献
4.
Totally 13575 ewes of two different breeds, Dala and Spel, were inseminated with semen, frozen in straws and thawed at 70°C for 8 sec. An insemination dose of 0.2 ml containing approx. 150 × 106 spermatozoa with at least 45 to 50% progressive motility was imerted 5 to 12 mm into the cervix. The insemination was performed once between 12 and 30 h after the onset of heat. The NR rate of the Dala ewes increased significantly during the season. The NR rate of the ewes inseminated before 15. November was 44.3%, from 15. to 20. November 52.2%. from 20. to 25. November 55.3% and from 25. November and later 61.4%. The corresponding values for the ewes of the Spel breed were 57.3, 58.7, 61.5 and 71.0% respectively, and only the difference between the two last values was statistically significant. The difference between the fertility of the two breeds was significant within each of the periods . 相似文献
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6.
通过对精液冷冻保存的细胞反应原理的阐述 ,指出冷冻保护剂甘油对精液保存具有利弊效应 ,提出精子膜脂组成的差异使得不同品种的精子对冷冻损伤的易感性不同。雌性生殖道解剖结构的品种差异 ,精子形态 ,精子运行机制的细微差异 ,人工授精时间及精子的运行能力 ,采精方式等因素对精液冷冻保存和人工授精的成功有决定性作用。研究精子质膜的生物学特性可解决低活力精子的问题 ,然而这并不能解决冷冻后精子质量的个体差异。对精细胞基因组的研究可以找出这些个体的遗传差异。因此 ,冷冻精子和精原细胞 (用于细胞外注射 )的差异已经成为完整基因组问题。 相似文献
7.
性别鉴定技术与人们的日常生活及畜牧业的发展有着密切的关系。精子性别鉴定技术的迅速发展为这一技术的应用提供了广阔的前景。目前最常用的精子性别鉴定技术是流式细胞分选法 ,此法依据 X-精子及 Y-精子 DNA含量的不同将两种精子分开 ,还可以用作分类后精子的检验。因流式细胞分选法存在一些不适于大规模应用的缺陷 ,人们希望能依据分类后的精子之间的对比研究找出特异的蛋白制成抗体 ,设计出高效免疫的精子性别鉴定技术 ,关于这方面的研究已经取得了一定的进展 相似文献
8.
维生素E对绵羊鲜精及冻精精液品质的影响 总被引:5,自引:0,他引:5
日粮中添加维生素E可以提高绵羊鲜精的活率 ,对照组和试验组的活率分别为 0 72± 0 0 7和 0 78± 0 0 6(P <0 0 5 ) ,显著改善鲜精精液品质。采用两步稀释法在绵羊冷冻精液稀释液中添加维生素E ,可以极显著降低冷冻对精子顶体的冷刺激损害程度 ,试验组的活率 ( 0 43 5± 0 0 2 0 )极显著高于对照组 ( 0 3 65± 0 0 2 6) (P <0 0 1) ,精子顶体的总异常率试验组 ( 3 3 72 % )极显著低于对照组 ( 4 4 3 5 % ) (P <0 0 1) ,其中试验组顶体膨胀率和脱落率与对照组分别为 1 2 8%±0 5 8%、2 3 0 8%± 1 45 %与 2 68%± 0 5 9%、2 8 96%± 3 14 %。冷冻精液品质显著改善。 相似文献
9.
专门化肉用种羊选定、引进后,通过适应性研究,在较大规模养羊业生产实践中。对现代集约化肉羊业的主要关键技术进行研究、完善和创新,组装集成并应用于生产。技术研究应用结果既为市场提供了大批专门化肉用种羊和优质安全的杂种肉羊,取得了高水平的研究成果及显著的经济效益和社会效益.又为我国目前正在迅速发展的集约化肉羊业提供了可靠的技术支持和理想的生产模式:从适宜的专门化肉羊品种到运用高效母羊快速扩繁技术.建立和完善有效的肉羊杂交利用体系,普遍应用种羊鲜、冻精生产大批肉用杂种羔羊,种植高产优质饲草及其科学加工调制和利用.建立严格兽医防疫制度.实行放牧 补饲或全舍饲的精细管理和集约化育肥,至生产优质、安全、标准肉羊,获得显著的经济效益和社会效益。 相似文献
10.
Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production
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M Ristanic Lj Stanisic M Maletic U Glavinic V Draskovic N Aleksic Z Stanimirovic 《Reproduction in domestic animals》2018,53(4):947-954
Foetal sex determination using polymerase chain reaction (PCR) in mammals is based on the amplification of gender‐specific foetal DNA sequences circulating in maternal blood. The bovine synepitheliochorial placenta does not allow a direct contact between the trophoblast and the maternal blood, resulting in difficult passage of foetal DNA and, consequently, its very small amounts in maternal bloodstream. Circulating cell‐free foetal DNA (ccffDNA) encompasses short nucleotide fragments (300–600 bp) in maternal circulation. The aim of this study was to assess this non‐invasive method in accurate prenatal sexing in early and late gestational periods in comparison with ultrasound diagnostics. As various DNA isolation and amplification methods were tested, their success in obtaining reliable results was evaluated. Two groups were tested, each consisting of 20 pregnant cows. Blood of a bull and a non‐pregnant heifer was the controls. Extraction of foetal DNA was accomplished by three different methods: using tubes with silicone membranes, a single‐tube extraction without silicone membranes and phenol–chloroform extraction. Following each extraction method, foetal DNA was amplified using PCR and real‐time PCR with both bAML and TSPY primers in a separate reaction. Positive results were obtained only after amplification of foetal DNA extracted with a single‐tube extraction kit. In comparison with ultrasound examination results and foetal gender recorded at birth, the sensitivity of the PCR test was 90% in Group I, but the technique failed to detect male foetuses in Group II. The real‐time PCR test sensitivity in Group I was 90% and in Group II 91.6%. 相似文献