2型猪链球菌的血清学鉴定

本试验首次证实了２型猪链球菌在我国的存在，采用玻片凝集、琼脂扩散及毛细管沉淀等３项试验，对１９９０年以来广东某猪场断奶仔猪暴发流行的败血症、脑膜炎及关节炎病猪中分离的１５株链球菌进行了血清学鉴定。结果表明：１５株链球菌均为２型猪链球菌；用高压法提取抗原进行沉淀反应，其结果比酸热法提取抗原具有简便、敏感、特异性强的特点。     

建立农业机械质量调查制度的探讨

随着我国农业机械保有量的不断增加,农业机械质量已经日益成为人们关注的热点问题.为此,依据<中华人民共和国农业机械化促进法>,以我国现阶段农业机械质量状况分析为基础,对农业机械质量调查工作和成果进行综述,从而进一步分析质量调查活动的特点以及优势,为农业机械质量调查方法研究提供必要性支持和理论依据.     

小麦跨区作业信息需求分析

根据2005年小麦跨区作业调研的结果,分析了机手的基本情况、跨区作业过程中的信息需求、信息获取方式、信息获取渠道等。建议建立一个短信息系统网络,通过手机短信来发送与传递信息,引导跨区作业可持续发展。     

Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

Hybrid wild boar husbandry is an important component of livestock production in Northeast China. However, the current disease situation of these animals is largely unknown due to a lack of disease surveillance. The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China. Between September 2015 to December 2016, 169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin, Inner Mongolia and Heilongjiang provinces. ELISA detected serum antibodies against classical swine fever virus(CSFV), porcine reproductive and respiratory syndrome virus(PRRSV), pseudorabies virus(PRV), porcine circovirus type 2(PCV2) and Japanese encephalitis virus(JEV), but not against African swine fever virus(ASFV), with PCV2 having the highest seropositive rate(87.2–100% in different farms). RT-PCR or PCR performed on the processed samples detected only PCV2, with 33.1%(56/169) of blood samples and 32.8%(20/61) of spleen samples being positive, respectively, indicating widespread PCV2 infection in hybrid wild boars. Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a, PCV2b and PCV2d, with nucleotide and deduced amino acid homologies of 88.5–100% and 88.1–100%, respectively.     

辽宁努鲁儿虎山自然保护区维管植物资源调查初报

通过对辽宁努鲁儿虎山国家级自然保护区维管植物资源进行的全面调查,结果表明：努鲁儿虎山自然保护区共有维管植物97科412属1 006种（变种、亚种和变型）,国家重点野生保护植物4种,中国珍稀濒危保护植物7种,辽宁濒危保护植物13种。     

建兰花叶病毒的分离,鉴定及检测研究

从石斛兰病株中获得一个病毒分离物，利用曼陀罗进行分离纯化和繁殖，并通过梯度离心获得提纯病毒，经生物学、血清学鉴定及电镜观察，鉴定该分离物属于建兰叶病毒。分别用纯化病毒及ＳＤＳ－ＰＡＧＥ回收病毒外壳蛋白免疫家兔。获得２种特异性抗血清，并用ＳＰＡ－ＥＬＩＳＡ方法对兰花样品进行了检测研究。     

Bluetongue Virus （BTV） Serological Survey and Evidence of Emergent BTV-8 Serotype in Morocco

Bluetongue （BT） is an infectious, non-contagious arthropod-borne disease that infects all ruminants, including sheep, cattle, deer, goats and camelids. Bluetongue virus （BTV） belonged to Reoviridae is ARN genome of 19 Kb. Twenty-six BTV serotypes have long been recognized, to be associated with severe disease in certain breeds of sheep, whereas cattle and goats are usually sub-clinically affected. Before 2004, BT was considered an exotic disease in Morocco, however, the first outbreak was observed in 2004 in sheep. This outbreak was caused by the isolated BTV-4. Two years later a BTV-1 emerged in Morocco. Both serotype 1 and serotype 4 circulated after 2007 across the country. The aims of the present work was to perform a serological study on sheep from different regions in Morocco in order to clarify the current BTV epidemiological situation and its evolution from 2009 to 2012, to determine the co-infection rate, and to confirm the possible circulation of other BTV Serotype mainly the BTV-8. All of 436 sera were tested by serum neutralization using reference strains. Results confirm the presence of BTV-4, BTV-1 and BTV-8. However, the present study report for the first time the emerging BTV-8 circulation in Morocco. Moreover, the founding reveal as well a higher co-infection rate in cattle compared to sheep.     

天敌对三化螟的捕食功能的血清法评价

试验用血清法检验了几种主要的捕食性天敌文对三化螟成虫和幼虫的捕食反应。化测定时间里,在秧田每天每亩成虫被蜘蛛捕食的量为9.94%,在大田每天每亩为620.2头。对幼虫的检验结果,转株幼虫有近1/10被捕食。结论是:蜘蛛类在三化螟成虫盛发期可起到一定的作用,对转株幼虫的杀伤作用较大。但由于天敌的数量消长和害虫的数量消长缺乏同步性,故达不到理想的控制效果。     

Research Progress on Rapid Serological Assays for Diagnosis of Japanese Encephalitis B

Japanese encephalitis B (JE) is a zoonosis caused by Japanese encephalitis virus which invades the central nervous system,infected by mosquito.Various animals could be the sower and reservoir of JE including swine,horse,cattle and avian.JE could cause a major public health trouble and a horrible crisis of the farm all in Asia and the Western Pacific region.Therefore,it is necessary to diagnose it rapidly and accurately.This review article made a sketch of approaches for the rapid serological assays of JE,including traditional method,emerging technologies and specific improvements of the assay,so as to provide references for the study of rapid,simple and accurate JE diagnose methods.