Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

湖南猪致病性大肠杆菌的分离及药物敏感性监测

从湖南47个规模化猪场发生典型仔猪黄、白痢猪只的63份腹泻病猪分离出63株大肠杆菌，其中12株作致病力试验均有致病性，对25种抗菌药物药敏试验，大肠杆菌对胺卡那霉素和头孢唑啉敏感，敏感菌株分别占98．41％和92．06％；多种抗生素产生耐药性，且为多重耐药。     

黄姜综合利用清洁生产工艺

通过浆渣分离技术和膜分离技术对黄姜皂素生产的原有工艺进行改进,可以提高收率,获得副产物酒精、干冰、膳食纤维,并且可回用酸解工序的盐酸,实现清洁生产的目的.     

基于独立分量分析的噪声消除技术

通过分析传统去噪方法的优缺点,引入基于独立分量分析的噪声消除方法,该方法不需要观测信号为确定性信号的前提假设,通过对加噪观测信号进行盲源分离,得到源观测信号,从而实现对噪声的消除。仿真结果证明基于独立分量分析的噪声消除方法,有效地去除了观测信号中的加性噪声。     

印楝种籽初制工艺技术装备的研制

本文对印楝种籽的加工机理进行了研究,提出了满足生产需要的工艺路线,研制出了差速螺旋齿双级对滚破壳设备、吸风分离设备,获得了较好的加工效果。     

馆员焦虑与馆员职业生涯

由于改革充满了不确定性和模糊性,加之馆员个体经验和能力差异,在面对复杂的变革情境时．会产生不同类型的焦虑,它影响着馆员的职业发展与图书馆改革的实施。因此,减少馆员的焦虑程度对推进馆员职业发展具有极其重要的意义。     

补骨脂抑菌活性成分分析及鉴定

本文以苹果腐烂病菌为指示菌,采用菌丝生长速率方法测定补骨脂的抑菌活性,其中提取物采用浸渍法提取,通过反复柱层析、薄层层析等分离纯化方法,对补骨脂粗提物的活性成分进行系统的分离,最终得到5种活性物质,经波谱分析法鉴定5种化合物分别为补骨脂酚（bakuchi01）、呋喃香豆素精（bakuchicin）、补骨脂查尔酮（bavachalcone）、补骨脂定（psoralidin）和补骨脂二氢黄酮（bavachin）。经过室内毒力试验,5种化合物的EC50分别为6．117、60．441、3．420、36．815和6．144mg·L-1,EC90分别为204．480、97．795、15．334、221．860和89．631mg·L-1,表明5种化合物对苹果腐烂病菌均具有很高的抑制活性。     

香根草挥发性成分提取分离及其农用生物活性研究进展

随着植物源农药的发展,具有优良农用活性的禾本科植物香根草Vetiveria zizanioides (L.) Nash越来越受到重视。文章首先介绍了香根草中挥发性成分的提取分离方法及结构鉴定,列举了其主要挥发物的结构;进而综述了其对二化螟 Chilo suppressalis (Walker) 等螟蛾科害虫的引诱作用、家白蚁Coptotermes formosanus Shiraki 等白蚁的驱避及拒食作用、对蚬木曲脉木虱Macrotermes barneyi Light等的毒杀作用,对水稻纹枯病菌Rhizoctorzia solani、香蕉炭疽病菌Calletotrichum musae等多种真菌和金黄色葡萄球菌Staphylococcus aureus Rosenbach 等多种细菌的抑菌活性,以及对藜Chenopodium album L.、豚草Ambrosia artemisiifolia L.等常见杂草的除草活性等;最后总结了香根草中活性化合物诺卡酮的化学及生物合成方法。可为进一步将香根草应用于植物源农药研究和开发利用提供参考。     

Periodical characteristics of baseflow in the source region of the Yangtze River

Baseflow,which represents the drainage of groundwater aquifers,is an essential component of runoff in hydrological basins.In the source region of the Yangtze River,the change of baseflow typically reflects the interactions between groundwater system and climatic factors in cold and arid areas.With modified Kalinen separation method,annual baseflow between 1957 and 2009 in this region was estimated and calculated.In comparison with the inner-annual variations of total streamflow,baseflow showed a weaker fluctuation.Before the 1980s,it was in a steady state;and after then,it demonstrated dramatic variations and large amplitudes.Based on the calculation results of baseflow,the real Morlet wavelet method was applied to reveal the periodical characteristics of baseflow as well as the precipitation and air temperature in the study area.It was found that annual baseflow has a 43-year trend as well as a 21-year period and a 7-year period.The 21-year period is most significant,with its wavelet coefficient having the largest fluctuation and amplitude.Summation of wavelet coefficients on these periods exhibits a similar change pattern with respect to that of annual baseflow.The summation curve takes a"W"shape,which means that the baseflow follows a four-stage sequence of descending–ascending–descending–ascending.As analyzed,the relationship among baseflow,precipitation and temperature is implied in the correlation between their normalized wavelet coefficients at different temporal scales.By the significant positive linear correlations both between precipitation and baseflow(correlation coefficient is 0.98)and between temperature and baseflow(correlation coefficient is 0.90)for the 43-year wavelet coefficients,it is suggested that the long-term increasing trends of precipitation and air temperature will lead to an increasing trend of baseflow.For wavelet coefficients of 21-year and 7-year periods,the positive linear correlation between precipitation and baseflow is significant.However,the correlation between air temperature and baseflow is not so evident,especially for the 21-year period.As a conclusion, correlation analysis with normalized wavelet coefficients showed that the change of annual baseflow was contributed mostly by the change of precipitation and secondly by the change of temperature.     

农用抗生素分离纯化的研究进展

目前我国农用抗生素大规模生产的品种主要有井冈霉素、阿维菌素和赤霉素,而未能大规模生产的抗生素共同存在的问题是技术不成熟,生产成本较高,进而限制了其在生产实践中的应用;另外我国农用抗生素研究的人力、物力过于分散.对一些优良品种投入太少,也是造成大规模生产的农用抗生素品种少的主要因素.阐述了国内外农用抗生素有效成分分离、提取、纯化技术特点与发展趋势.