IMAGING DIAGNOSIS: MAGNETIC RESONANCE IMAGING OF A CERVICAL WOODEN FOREIGN BODY IN A DOG

This article describes the discovery of a chronic cervical wooden foreign body ventral to the left transverse processes of the cranial cervical spine using magnetic resonance imaging (MRI) in a dog that presented with chronic neck pain and lameness. The dog did not exhibit dysphagia or chronic draining tracts, the most common signs of the presumed cause, that of a penetrating oropharyngeal foreign body. The foreign body itself was represented on MR images as an oval straight-edged core within an inflammatory tissue reaction. The wood was slightly hyperintense on T2- and isointense on T1-weighted images relative to muscle. Surrounding this was a more conspicuous contrast-enhancing reactive tissue rim that was hyperintense on all pulse sequences. Adjacent musculature also exhibited diffuse edema and contrast enhancement that extended around the left cervical vertebral transverse processes and local intervertebral nerve roots. The foreign body was found to be a wooden stick upon surgical removal. MRI is an excellent method for visualizing the inflammatory tissue reactions associated with soft-tissue foreign bodies because of its contrast resolution and depiction of anatomy in multiple imaging planes.     

Validating the effectiveness of alternative euthanasia techniques using penetrating captive bolt guns in mature swine (Sus scrofa domesticus)

Euthanasia of mature swine is challenging. Temporal and behind-the-ear locations are two sites that have been identified as alternatives to the more commonly used frontal placement. In stage one, the effectiveness of two penetrating captive bolt gun styles (cylinder or pistol) was evaluated using frontal, temporal, and behind-the-ear placement in anesthetized mature swine (n = 36; weight: 267 ± 41 kg). For stage one, when evaluating treatment efficacy by sex, the cylinder-style equipment was 100% effective in achieving death when applied to all cranial locations (frontal, temporal, and behind-the-ear) for sows; however, the pistol-style equipment was only 100% effective when applied at the behind-the-ear location for sows. For boars, the cylinder-style equipment was 100% effective when applied to the frontal and behind-the-ear location, but the pistol-style equipment was not effective for any cranial location in boars. Therefore, the pistol–frontal, pistol–temporal, pistol–behind-the-ear, and cylinder–temporal were not included for boars, and pistol–frontal and pistol–temporal were not included for sows in stage two. In stage two, commercial, mixed-breed, mature swine (n = 42; weight: 292 +/− 56 kg) were randomly assigned to one of four treatments based on the inclusion criteria described in stage one. A three-point traumatic brain injury (TBI) score (0 = normal; 1 = some abnormalities; 2 = grossly abnormal, unrecognizable) was used to evaluate six neuroanatomical structures (cerebral cortex, cerebellum, hypothalamus, thalamus, pons, and brain stem), and the presence of hemorrhage was also noted. All treatments were 100% effective in stage two. A significant interaction between gun style and placement was determined on predicting total TBI as the cylinder style produced a higher total TBI score compared with the pistol type of the magnitude of +2.8 (P < 0.01). The cylinder style tended to produce a greater TBI score than the pistol in the temporal location (+1.2; P = 0.08). No difference was noted for TBI score behind-the-ear between the cylinder- and pistol-style gun (P > 0.05). TBI tended to be less in boars compared with sows (−0.6; P = 0.08). Hemorrhage was observed in frontal, parietal, occipital, and temporal lobes. This study demonstrated that the cylinder-style captive bolt gun more effectively resulted in brain trauma and death compared with a pistol-style gun and the behind-the-ear and temporal placement showed promise as an alternative placement site for euthanizing mature pigs on-farm.     

探地雷达树木根系定位与直径估算

针对树木根系的探地雷达(Ground Penetrating Radar,GPR)检测图像复杂、解译困难、自动化程度低且精度不高等问题,该研究提出了一种基于YOLOv3的树根自动识别和参数估计的方法。通过不同的根预埋试验分析了根直径、掩埋深度和朝向对根系识别和预测的影响,对比评估了在复杂现场环境下该方法与商业软件常用的阈值分割方法的识别效果。结果表明算法可实现根系自动提取和双曲线顶点定位,对根双曲线的识别准确率和召回率分别达到了96.62%和86.94%,根系参数预测的总平均相对误差在10.57%以内。该方法具有较高的识别准确率和鲁棒性,可实时地对树木根系进行检测并进行根系参数预测,对树木根系无损检测具有重要意义。     

基于修正介电常数模型的煤矿区复垦土壤压实评价

模拟复垦机械对试验区土壤进行不同程度的压实,在探地雷达获取土壤介电常数的基础上,检验并修正了4种经典复合介电常数模型,并结合野外试验对修正后的模型通用性进行了验证。结果表明,土壤介电常数与土壤压实指标(土壤紧实度、容重、孔隙率等)相关系数89%,可以表征土壤压实状况;原有复合介电常数模型虽然存在误差不能直接使用,但模型计算值与实测值相关系数0.99,修正后二者拟合误差1%;在野外试验中,基于修正后的介电常数模型反算的土壤压实指标(容重、孔隙率)与实验室测量值误差率5%,通用性较好。表明在科学布设测线以保证探地雷达准确获取土壤介电常数的前提下,可以通过修正后的复合介电常数模型对煤矿区复垦土壤压实状况进行全面客观的评价。     

细胞穿透肽介导的小黑麦小孢子遗传转化研究

本研究选用小黑麦小孢子作为供试材料,进行细胞穿透肽介导的植物单倍体细胞遗传转化可行性研究。提取单核靠边期的小黑麦小孢子进行悬浮培养,将细胞穿透肽与报告基因GFP的表达框复合物对小孢子进行转化。转化植株进行了PCR分子检测,并在荧光体视显微镜下观察外源GFP基因的表达情况。共获得21株候选转基因植株,其中染色体自然加倍植株为10株。T1代加倍植株的PCR检测结果显示,加倍的转基因株系中有3个株系的PCR检测结果呈阳性。通过荧光体视显微镜可在转基因植株的幼胚部位观测到GFP蛋白的荧光。以上结果表明外源GFP基因已经整合到小黑麦基因组上并且得到表达并稳定遗传。因此,由细胞穿透肽介导的植物单细胞转化是一种有效、可行的植物单细胞转基因方法。     

木材流体渗透理论与研究方法

为较全面系统地了解木材流体渗透理论研究现状,从流体在多孔介质内的主要类型与流动理论、流体在木材内的主要流通通道及木材流体渗透性研究的主要理论与试验方法等几方面进行综合概述,提出木材流体渗透性理论研究的主要发展方向.试验观察应采取更先进的手段,动态观察流体尤其是液体渗透木材的整个连续过程,并构筑更合理的木材流体简化结构渗透模型,以较少的参数反映更完整的流体渗透途径;理论研究应借鉴木材干燥过程中应用较成熟的传质传热模型,建筑多孔材料如水泥、石、砖等的渗透性研究,土壤流体渗透性及地下石油流体渗透性研究方面应用较成熟的理论和方法,并拓宽木材渗透性的研究对象,建立符合木材非稳态渗透实际过程的二维或三维理论模型,以更逼真地模拟木材流体渗透过程;2种方法结合起来,定性和定量完整描述木材流体渗透机制.     

诱导栉孔扇贝雌核发育时精子入卵的扫描电镜观察

取栉孔扇贝 (Chlamysfarreri)精子在 80 0 μW/(cm2 ·s)的紫外线下辐射 ,然后与正常卵子受精 ,每隔 0 .5min取“受精卵”固定。在扫描电镜下连续观察精子的入卵过程 ,然后与正常精子的入卵过程进行比较。结果表明 ,紫外辐射后的精子可以划分为 3种类型 :I类精子 ,基本无明显变化 ,可以激发卵黄膜举起形成受精膜 ,同时诱发卵子发生皮层反应 ,在受精锥的作用下正常入卵 ,但入卵的时间较正常精子晚 ;II类精子 ,顶体膜破裂 ,顶体丝伸出 ;III类精子 ,顶体丝伸出 ,鞭毛脱落。由于II、III类精子在紫外辐射条件下诱发了顶体反应 ,融解卵膜的酶提前释放 ,因而不能入卵。精子鞭毛的脱落 ,使精子丧失了运动能力 ,是导致卵表面附着的精子数量较少的原因之一。无论是正常精子还是辐射后的精子 ,入卵后在卵表面都留下一个类似受精孔的通道。实验组中未发现有多精入卵现象     

Analysing the Rockmass Permeation of a Low-medium Radioactive Waste Disposal Field

The fractures distribution and fracture rock mass permeation research is the premise condition that to study groundwater movement and pollutant migration in fracture rock mass in bed rock area, which includes the dominance orientation of fracture that groundwater fluxes in it, the brisk zone distribution of fracture water and the permeability calculation of rock mass. This paper mainly analyzes the fracture distribution and the dominance orientation of fracture and the permeability of a low-medium radioactive waste disposal field based on its rich geologic record. The following results are found through Statistical analysis and comparison calculation.The active zone of fractural water in bed rock is an active channel of active nucleus migration. Tthe headrace fracture of disposition have a dominant orientation. The penetrating coefficient tensor in bed rock fracture is also calculated.     

人参黑斑病的研究——Ⅰ.损失调查、病原菌及发病规律

人参黑斑病可使参根减产0.4～1.6倍;重者达10倍以上。人参黑斑病菌Alternaria panax whetz.原取名西洋参;经测定辽宁的分主孢子尺度明显偏大。接种和调查29种杂草,在参场内未见其它奇主.分生孢子萌发的起点温度为1℃±;最适为20～25℃。孢子以在人参叶汁、1％糖水、露水和饱和湿度下萌芽率最高:在蒸馏水、自来水中萌芽率最低;土壤对孢子萌发有强烈抑制作用。病害的侵染来源是分生孢子,以穿透方式侵入,潜育期24～48小时,接种3～6天产生孢子,且病部可反复多次产生,每平方厘米病叶可产孢4,107～51,120个;越冬病叶也可再次产生孢子和造成侵染。孢子没有休眠期,随时可以萌发侵染,但不能为害木质化的茎秆。黑斑病具有小区内局部流行特点。孢子体形大,浮动传播较差,田间菌源的多少与发病成正相关。据辽宁的气象条件,人参产区是黑斑病的长发区。