THE INSENSITIVITY OF 99mTc PERTECHNETATE FOR DETECTING METASTASES OF A FUNCTIONAL THYROID CARCINOMA IN A DOG

This report describes the use of ^99mtechnetium pertechnetate (^99mTcO₄⁻) and ¹³¹[for imaging of a metastatic thyroid carcinoma in a dog. The ¹³¹] imaging showed metastatic lesions undetected by the ^99mTcO₄⁻ imaging on 2 separate occasions. The possible mechanisms for the discrepancies between ¹³¹I and ^99mTcO₄⁻ imaging of thyroid carcinomas are discussed. The use of ¹³¹I for the imaging of functional thyroid carcinomas in the dog is recommended.     

Results from the treatment of advanced stage squamous cell carcinoma of the nasal planum in cats, using a combination of intralesional carboplatin and superficial radiotherapy: a pilot study

Six cats with an advanced stage squamous cell carcinoma (SCC) of the nasal planum were treated with a combination of superficial radiotherapy and intralesional carboplatin therapy. This multimodality protocol was well tolerated by the majority of cats and resulted in complete responses in all cats (100%). Median follow‐up for all cats is 268 days, and the median time‐to‐recurrence, time‐to‐progression and overall survival have not yet been reached. Our study, although limited in number of animals and with a relatively short median follow‐up compared to other studies for this disease, suggests that a combination of radiotherapy and intralesional carboplatin is a useful treatment option for an advanced stage SCC of the nasal planum in cats and warrants further application of the multimodality approach presented here.     

Dihydroartemisinin enhances radiotherapy efficiency in H22 cell tumor-bearing mice by regulating PI3K/AKT signaling pathway

AIM To study the effect of dihydroartemisinin （DHA） on the radiotherapy efficiency in hepatocellular carcinoma H22 cell tumor-bearing mice and the role of phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway in this process. METHODS A model of H22 cell tumor-bearing mice was established. The mice was divided into model group, single radiotherapy group, 5-fluorouracil （5-FU） group, and low-, medium- and high-dose DHA groups. The body weight and tumor volume in each group were measured every other day. At the end of administration, blood was collected from the tail of the mice and the animals were killed by neck removal immediately. The synergistic effect of DHA on radiotherapy was determined, and tumor growth inhibitory rate was calculated. The degree of lymphocyte transformation and natural killer （NK） cell activity were measured by MTT, the serum levels of interleukin-2 （IL-2） and IL-4 were measured by ELISA, and the protein levels of PI3K, AKT and p-AKT were determined by Western blot. RESULTS The H22 cell tumor-bearing mouse model was successfully constructed. Compared with model group, the TGT₃ （tumor growth time to reach 3 times of volume） of single radiotherapy group was remarkably increased （P<0.05）, while tumor weight, lymphocyte transformation degree, NK cell activity, IL-2 and IL-4 levels, PI3K protein level and AKT phosphorylation level were remarkably decreased （P<0.05）. Compared with single radiotherapy group, TGT₃, EF （enhancement factor）, tumor inhibitory rate, lymphocyte transformation degree, NK cell activity, IL-2 level and IL-4 level were increased with the increase in DHA dose （P<0.05）, and the PI3K protein level and AKT phosphorylation level were decreased （P<0.05）. CONCLUSION DHA may enhance the immunity of tumor-bearing mice by inhibiting the activity of PI3K/AKT signaling pathway, thereby enhancing the efficacy of radiotherapy.     

洛伐他汀影响人高转移卵巢癌细胞系HO-8910PM转移相关能力的研究

目的研究洛伐他汀对人高转移卵巢癌细胞系HO-8910PM转移相关能力的影响。方法分别运用MTT法、Transwell小室法、细胞粘附人工重组基底膜实验检测洛伐他汀对HO-8910PM细胞的细胞毒作用,对细胞的侵袭能力和趋化运动及对细胞粘附能力的影响。结果洛伐他汀作用HO-8910PM细胞6h后能抑制其体外侵袭、趋化运动和粘附能力,其中8μmol/L抑制率分别为(79.18±0.21)%、(59.40±0.80)%和(14.08±1.28)%。结论洛伐他汀能有效地抑制人高转移卵巢癌细胞系HO-8910PM的侵袭、趋化运动和粘附能力,是潜在的抗肿瘤转移药物。     

Reconstruction of the lower eyelid by third eyelid lateral advancement and local transposition cutaneous flap after "en bloc" resection of squamous cell carcinoma in 5 cats

OBJECTIVE: To describe a novel technique for blepharoplasty to cover a tissue defect involving >/=50% of the lower eyelid. STUDY DESIGN: Prospective clinical study. ANIMALS: Five cats with lower eyelid squamous cell carcinoma (SCC). METHODS: En bloc resection of SCC by removing >/=50% of the lower lid with either the medial or lateral canthus was performed without other adjunctive treatment for SCC. The lid defect was reconstructed with a transposition skin flap derived from the frontal (medial defect) or temporal (lateral defect) region. The third eyelid was advanced laterally without dissection from its insertion; its outer conjunctival layer was removed, and the skin flap was sutured with single interrupted sutures dorsally over the nictitating membrane, ventrally to the cutaneous edge of the surgical wound and medially or laterally (depending on the canthus removed) to the skin of the remaining lower lid. RESULTS: Satisfactory cosmetic and functional results were achieved and the Schirmer tear tests were normal. In 2 cats, the skin flap needed monthly hair trimming to avoid corneal lesions. CONCLUSIONS: After en bloc resection of SCC involving >/=50% of the lower eyelid, reconstruction can be achieved by relocation of the third eyelid and use of a cutaneous transposition flap sutured to the scarified external surface of the third eyelid. Eyelid apposition and lacrimal function were preserved. CLINICAL RELEVANCE: Blepharoplasty using a cutaneous transposition flap sutured to the scarified surface of a relocated third eyelid should be considered for reconstruction of lower eyelid defects with >/=50% tissue loss of the lid margin.     

饲粮非植酸磷水平对39~46周龄黄羽肉种母鸡繁殖性能、胫骨指标和血浆生化指标的影响

本试验旨在研究不同非植酸磷( NPP)水平对39~46周龄黄羽肉鸡父母代种鸡繁殖性能、胫骨指标和血浆生化指标的影响,探讨该阶段适宜磷供给量。试验选用39周龄岭南黄羽肉鸡父母代种母鸡810只,根据体重和产蛋率分为5个组,每组6个重复,每重复27只鸡。组1为对照组,饲喂玉米-豆粕型基础饲粮( NPP水平为0.30%),组2、3、4和5饲粮分别在基础饲粮中添加不同水平的磷酸氢钙,使饲粮NPP水平分别为0.35%、0.40%、0.45%和0.50%,其他营养成分各组基本一致。试鸡限饲122 g/(只·d),自由饮水。试验期8周。结果表明：1)试验期内, NPP水平未显著影响试鸡平均日增重、平均日产蛋量、料蛋比、平均蛋重和破蛋率(P>0.05),显著影响了产蛋率和不合格蛋率(P<0.05),0.45%组产蛋率显著高于0.30%和0.40%组(P<0.05),0.40%组不合格蛋率显著低于其余4组(P<0.05)。2)饲粮 NPP水平显著影响了试鸡39~40周龄种蛋长径、蛋短径、蛋形指数和蛋壳强度(P<0.05),显著影响了45~46周龄种蛋蛋壳强度( P<0.05)。3) NPP水平对种蛋孵化性能(受精率、孵化率、健雏率、健雏平均出壳重)无显著影响(P>0.05)。4)饲粮 NPP 水平显著增加了试鸡脱脂胫骨粗灰分中钙、磷含量(P<0.05)。5)0.40%和0.45%NPP组试鸡血浆甲状旁腺激素含量显著高于0.30%和0.35%组( P<0.05),0.50%组血浆降钙素含量显著高于其余4组( P<0.05)。综上,39~46周龄黄羽肉鸡父母代种母鸡饲粮NPP适宜水平为0.40%~0.45%,根据采食量计算得到每日NPP需要量为0.488~0.549 g。     

Diagnosis and Treatment of Anaplastic Mammary Carcinoma in a Sugar Glider (Petaurus breviceps)

A 9-year-old female sugar glider (Petaurus breviceps) was evaluated for a tissue mass near the marsupium. Ultrasonography identified a vascular mass originating from the right mammary gland. Fine-needle aspiration was suggestive of a malignant neoplasm. The glider was anesthetized and the tumor was removed, and this was followed by strontium-90 plesiotherapy to the tumor bed in an attempt to decrease local recurrence. Histopathology revealed an anaplastic mammary carcinoma. The glider was euthanized less than 14 days after the procedure owing to self-mutilation behavior of unknown etiology. This report is the first to describe the clinical presentation, diagnostics, therapeutics, and treatment response for a sugar glider with mammary neoplasia.     

Ovarian mixed germ-cell tumor comprising mature teratoma and embryonal carcinoma in a four-toed hedgehog (Atelerix albiventris)

This report describes the clinical and histopathological characteristics of a rare mixed germ-cell tumor comprising teratoma and embryonal carcinoma in the left ovary of a 10-month-old four-toed hedgehog, with chief complaints of loss of appetite and lethargy. Laparotomy revealed a swollen left ovary with small disseminated peritoneal nodules, and bilateral ovariohysterectomy was performed. The left ovary had a mature teratoma with well-differentiated fat, bone, cartilage, salivary gland, trachea, keratin cyst, and nervous tissues, and an embryonal carcinoma consisting of poorly-differentiated epithelial cells arranged in tubular, alveolar, or solid patterns. Immunohistochemically, the embryonal carcinoma cells were positive for placental alkaline phosphatase and c-KIT. This is the first case of mature teratoma with embryonal carcinoma in the ovary of a hedgehog.