江西省数字农业发展现状、问题与建议

数字农业的发展对于农业现代化转型升级具有重要意义。根据江西省县域数字农业相关数据和文献资料,分析江西省数字农业发展现状,指出目前江西省数字农业发展存在数据服务配套不完善、小农户应用困难以及法律法规政策建设相对落后等问题,提出应加强数字农业信息服务支持力度、培育新型农业经营主体,以及强化政策顶层设计等建议。     

基于无人机航摄技术在土地整治项目中的应用研究

针对传统土地整治项目施工监管、后期监测效率不高的问题,本文通过低空无人机航摄和PhotoScan软件后期处理,对比生成的正射影像图和Google earth卫星图的分辨率和位置,发现该影像图能精准、高效的表征项目区影像数据变化,从而得到一种可用于土地整治项目各阶段动态观测的简单、快捷方法。     

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.     

Zerumbone attenuates MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells by inhibition of oxidative stress

AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP⁺-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP⁺ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP⁺ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP⁺-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis.     

CRISPR/cas系统及其在家禽上的应用研究进展

CRISPR/cas是一种获得性免疫防御系统,广泛存在于细菌和古细菌中。Strep tococcus pyogenes cas9(Spcas9)作为目前研究最多、最清楚的效应蛋白,因其酶活性高和靶点广而被应用于生命科学各个领域,但Spcas9分子量大、脱靶率高等缺点在一定程度上限制了其应用。随着CRISPR系统技术被深入开发,一些新效应蛋白被发现,本文就CRISPR/cas系统分类、原理、新效应蛋白发现及cas9(S.Pyogenes)多种变构体技术开发在及家禽上的应用进行综述,为相关领域的研究提供参考。     

JT6120乘客座椅靠背曲线静态舒适性评价研究

The aim of this research was to evaluate a passenger seat of a new type bus in terms of eomfort. A fuzzy set model of multistage comfort scale (MCS) was adopted to the assessment of comfort, together with the techniques of human backshape and EMG measures     

交沙霉素等四种药物对雏鸡隐孢子虫病的预防效果

对经口感染(4.0×10~5个/鸡)贝氏隐孢子虫(Cryptosporidium baileyi)卵囊的雏鸡在其饲料中分别添加交沙霉素(4.0g/kg)、复方新诺明(4.9g/kg)、大蒜素(400mgkg/)、甲硝唑(2.0g/ks),任鸡自由采食,连用7d.结果表明,实验鸡潜隐期延长,粪中卵囊数显著降低,体重明显增加,血液学值及血清酶学指标与健康对照鸡无明显差异.由此证明,这4种药物对实验性雏鸡隐孢子虫病有不同程度的预防作用.