体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。     

脑活素治疗新生大鼠缺血缺氧性脑病机理的研究

7日龄Wister大鼠结扎左侧颈总动脉后吸入氧氮混合气体（8％O2和92％N2）2h,制成缺血缺氧性脑病动物模型,测定大鼠脑组织中丙二醛（MDA）的含量和超氧化物歧化酶（SOD）的活性。结果显示,缺血缺氧后6h,缺血缺氧组MDA含量显著升高,24h达到高峰,以后逐渐下降,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组MDA含量于缺血缺氧后6h已经显著下降,72h与对照组比较无统计学差异。缺血缺氧组SOD活力值在缺血缺氧后6h已经下降,24h降到最低点,以后逐渐回升,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组SOD活力值在缺血缺氧后6h明显提高,于缺血缺氧后96h与对照组比较无统计学差异。结果提示,缺血缺氧引起新生大鼠脑组织的氧化-抗氧化系统失衡,氧自由基大量产生,参与了新生大鼠缺血缺氧性脑病脑损伤过程;脑活素能够抑制氧自由基的生成,提高SOD的活力值,对缺血缺氧性脑病具有神经保护作用。     

The redox potential of growing cultures of Streptococcus bovis Orla-Jensen compared with other facultative anaerobes.

The changes of redox potential were measured in growing cultures of three strains of Streptococcus bovis, together with three strains of Staphylococcus aureus and one strain of each of Lactobacillus plantaram, Lactobacillus casei, and Eschericia coli. It was found that both S. aureus and E. coli could reduce the redox potential of the growth medium to very low values (between —400 mv and —600 mv), whereas the streptococci and lactobacilli were able to cause only slight or insignificant changes of the redox potential. Respirometric measurements confirmed that the capacity of oxygen consumption of S. bovis was very small compared to that of E. coli and S. aureus. On this basis the authors conclude that S. bovis in all probability is unable to contribute significantly to maintenance of the low redox potential of its natural habitat, the rumen. This function must be carried out by other bacteria, such as enterobacteria or staphylococci, which are capable of performing a true, aerobic respiration.     

Colour Flow Mapping examination: An useful screening test for the early diagnosis of ductus venosus patency in canine newborns

Ductus venosus (DV) closure plays a key role in hepatic circulation adaptation to postnatal metabolic function, and DV patency might develop a congenital portosystemic shunt (CPSS). The noninvasive Color Flow Mapping (CFM) examination, a validated method to diagnose CPSS in adult dogs, is routinely performed to assess DV closure after birth in humans. This study aimed to describe the feasibility of the ultrasonographic evaluation of the DV after birth and to determine its closure time in healthy Great Dane neonates. Patency of DV in serial Color Flow Mapping (CFM) examinations and bodyweight (BW) were recorded on Days 0‐3‐6‐9 in 24 neonates that were classified as having patent (PDV) or closed ductusvenosus (CDV) basing on CFM signal presence/absence. Since the 3rd day, DV diameter was recorded. Data were analysed by ANOVA (p < 0.05). All dogs resulted healthy 1 year later. The number of PDV and CDV puppies at birth was not different on Day 3 (24 and 0 vs. 22 and 2, PDV and CDV, respectively), whereas it resulted different on Days 6 (24 and 0 vs. 14 and 10) and 9 (24 and 0 vs. 0 and 24); on Day 3, it was different compared to Days 6 and 9; on Day 6, it was different from Day 9. Reduction of DV diameter resulted positively related to neonatal BW growth. The CFM evaluation of DV closure after birth in Great Dane puppies represents a feasible technique. Present results suggest the time of functional closure in normal neonates within 9 days after birth. Thus, CFM examination, as an early screening test for DV patency evaluation, performed 10 days after birth, may identify suspicious dogs at risk that would require further investigations. Further studies are needed to deepen the role of a delayed closure in low bodyweight and preterm puppies.     

瘤胃液移植在反刍动物中的研究进展

瘤胃液移植是一种将健康供体动物的瘤胃液转移给受体动物的技术。目前，针对瘤胃液移植的报道还相对有限，但越来越多的研究结果显示，瘤胃液移植可以影响反刍动物的干物质采食量、产奶量等，还具有重建瘤胃菌群结构的潜力。本文综述了瘤胃液移植的方法及实践中的应用研究，分析其对反刍动物生长性能、瘤胃发酵、瘤胃微生物区系的影响以及在治疗某些疾病方面的作用，为反刍动物瘤胃液移植的研究提供参考。     

Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand

ABSTRACT

Case history and clinical findings: A flock of 20 sheep was kept within three paddocks on a single property. None of the animals in the flock had been vaccinated against any disease for at least three years. Abdominal bloating and haemorrhagic diarrhoea were observed in Lamb 1 at 24 hours-of-age. The lamb subsequently died within an hour of the onset of clinical signs. Lamb 2 was 3-days-old when observed to be recumbent with opisthotonus. The lamb was treated with dextrose, vitamins B1 and B12, and penicillin G, but died 4 hours later.

Pathological findings: Examination of Lamb 1 revealed markedly increased gas within the peritoneum and within dilated loops of intestine. The intestines were dark red and contained large quantities of haemorrhagic fluid. Histology of the intestines revealed peracute mucosal necrosis with minimal accompanying inflammation. The intestinal lumen contained cell debris, haemorrhage, and myriad large Gram-positive bacilli. The intestines of Lamb 2 did not appear bloated or reddened. However, multiple fibrin clots were visible within the pericardial sac. Histopathological examination revealed small foci of necrosis within the mucosa of the distal intestine. The necrotic foci were often associated with large numbers of large Gram-positive bacilli.

Immunohistochemsitry and molecular biology: Intestinal samples from Lamb 1 were processed for Clostridium perfringens immunohistochemistry, which revealed large numbers of intralesional, positively immunostained rods. Fragments corresponding to the expected sizes for genes encoding alpha, beta, and epsilon C. perfringens typing toxins were amplified by PCR from DNA extracted from formalin-fixed sections of intestine.

Diagnosis: Lamb dysentery due to C. perfringens type B.

Clinical relevance: C. perfringens bacteria have a worldwide distribution, but disease due to C. perfringens type B has only been diagnosed in a small number of countries and has never been reported in New Zealand or Australia. C. perfringens type B produce both beta toxin and epsilon toxins, therefore both haemorrhagic enteritis and systemic vascular damage can develop. As many animals are exposed to C. perfringens without developing disease, there must be additional unknown factors that resulted in disease in these particular sheep. Vaccines that specifically protect against C. perfringens type B are available and may be recommended for use in smaller non-commercial flocks, as in the present case.     

向日葵副产物的营养特性及在反刍动物中的应用

饲料资源缺乏一直以来都是制约中国畜牧业发展的主要因素。从长远看，常规饲料已不能维持中国畜牧业的可持续发展，开发新的饲料资源是解决饲料不足的重要途径之一。向日葵副产物营养价值较高、来源广、价格低，是一种非常具有开发价值的新型饲料。向日葵副产物包括向日葵饼粕、向日葵盘、向日葵秸秆及葵花籽壳：向日葵饼粕蛋白质含量高、纤维含量低，是一种优质的植物蛋白来源，可以提高奶牛乳汁的营养价值、促进肉牛生长、提高羊的瘤胃发酵效果；向日葵盘粗脂肪含量高，含有大量的膳食纤维，可以提高奶牛的产奶量和乳脂率，促进肉牛增重，提高羊瘤胃挥发性脂肪酸浓度以及粗蛋白质、粗纤维、中性洗涤纤维的消化率；向日葵秸秆富含氮、磷、钾、钙、镁等矿物质元素以及少量的蛋白质，可以为肉牛提供一些常量元素和微量元素，提高羊的日增重，降低料重比；葵花籽壳的主要成分是纤维素和木质素，含有丰富的生物活性物质，可以提高奶牛日增重、肉牛饲料转化率。作者具体介绍了向日葵副产物饼粕、葵盘、秸秆、葵花籽壳的营养价值，以及目前它们在反刍动物饲料中的应用情况，以期为今后向日葵副产物的开发利用提供理论依据。     

Of rodents and ruminants: a comparison of small noncoding RNA requirements in mouse and bovine reproduction

Ruminants are major producers of meat and milk, thus managing their reproductive potential is a key element in cost-effective, safe, and efficient food production. Of particular concern, defects in male germ cells and female germ cells may lead to significantly reduced live births relative to fertilization. However, the underlying molecular drivers of these defects are unclear. Small noncoding RNAs, such as piRNAs and miRNAs, are known to be important regulators of germ-cell physiology in mouse (the best-studied mammalian model organism) and emerging evidence suggests that this is also the case in a range of ruminant species, in particular bovine. Similarities exist between mouse and bovids, especially in the case of meiotic and postmeiotic male germ cells. However, fundamental differences in small RNA abundance and metabolism between these species have been observed in the female germ cell, differences that likely have profound impacts on their physiology. Further, parentally derived small noncoding RNAs are known to influence early embryos and significant species-specific differences in germ-cell born small noncoding RNAs have been observed. These findings demonstrate the mouse to be an imperfect model for understanding germ-cell small noncoding RNA biology in ruminants and highlight the need to increase research efforts in this underappreciated aspect of animal reproduction.