鸭疫里默氏杆菌病发病机理的研究Ⅱ.人工感染雏鸭病理形态学变化

以实验病理学方法对人工感染Ⅰ型鸭疫里默氏杆菌(含菌量3×109CFU/mL)的20日龄天府肉鸭进行了系统病理组织学研究.眼观特征为全身浆膜及各组织器官的被膜纤维素渗出物;心脏、肝脏、脾脏肿大,胸腺、法氏囊缩小,脑水肿.病理组织学变化表现为纤维素性心包炎,肝周炎、气囊炎、脾炎和脑膜炎,腺胃和小肠出血性或坏死性炎,胰腺细胞、肌胃黏膜上皮及砂囊腺上皮细胞变性与坏死,肾小管变性与局灶性坏死,胸腺和法氏囊淋巴细胞减少.结果表明鸭疫里默氏杆菌病侵害多种组织器官,引起功能障碍致雏鸭发病死亡.     

鸭源隐孢子虫种类鉴定及对鸡鸭致病性研究

为鉴定在河南地区鸭群中广泛存在的隐孢子虫种类及其致病性，我们将河南省鸭源隐孢子虫分离株传代增殖，10只鸡和10只鸭分别逐只接种上述分离株640万个卵囊，应用光镜和扫描电镜技术对样品进行观察。根据卵囊形态测定数据，排卵囊规律，寄生部位及其寄生特点鉴定该分离株为贝氏隐孢子虫（Cryptosporidium baileyi)。该分离株接种鸡、鸭后主要引起呼吸道和法氏囊的炎症。病理形态学变化表现为气管纤毛脱落，杯状细胞排空，上皮细胞肥大或增生。除虫体寄生处之外，其他区域微绒毛尚保持完整。对虫体寄生密度，临床症状和病理变化综合比较的结果表明鸡对贝氏隐孢子虫易感性比鸭高。     

Morphopathological features of a severe ulcerative disease outbreak associated with Tenacibaculum maritimum in cultivated sole, Solea senegalensis (L.)

This study describes morphopathologic changes in naturally infected farmed Senegalese sole affected by tenacibaculosis caused by Tenacibaculum maritimum. Macroscopic observation, in addition to light microscopy and scanning electron microscopy, was used to study the lesions. Main lesions were characterized by complete loss of epidermis and dermis, as well as extensive necrosis of muscle layers. Mild-to-moderate inflammatory response with the presence of macrophages was noted around hyaline degenerated muscle cells. Gram-negative filamentous bacteria could be detected only at the dermis. Under scanning electron microscopy, filamentous bacteria located over the scales without epithelium could be observed. These findings together with the isolation and PCR detection of the bacteria in kidney and skin tissues suggest that once the bacteria reach the dermis, probably through eroded epidermis, they are able to proliferate and produce enzymes that are responsible for the damage in the underlying tissues.     

稚鳖粘液性肠炎病的病理形态学研究

稚鳖粘液性肠炎病的主要眼观病变是病鳖肛门排出粘液块,组织病理变化主要为肠腺分泌亢进,肠上皮细胞脱落,肠壁充血,肝脏脂肪变性,脾脏炎性细胞浸润,肾脏肾小管上皮细胞水泡变性,肺也有不同程度的病变。电镜下观察,发现随着病程的延续肠道的病变相应地加重,主要表现为炎性细胞浸润,上皮细胞变性和坏死。