真核生物核糖体和mRNA翻译的研究进展

文中就真核生物核糖体和mRNA翻译的研究进展进行了综述,并对其未来研究发展进行了展望。     

Decreased ACTH secretion during prolonged transportation stress is associated with reduced pituitary responsiveness to tropic hormone stimulation in cattle

The present study examined the effect of transportation stress on hypothalamic–pituitary–adrenal axis responsiveness to tropic hormone stimulation and on abundance of corticotropin releasing factor (CRF) receptor R1 (CRFR1) and arginine vasopressin (AVP) receptor V3 (V3) mRNAs in the anterior pituitary (AP) of cattle. Holstein steers were transported for 10 h or used as non-transported controls (NTC). Blood samples were collected at start of transportation and every 1–2 h thereafter. To test AP responsiveness to tropic hormones, animals were challenged (i.v.) with CRF (0.5 μg/kg), AVP (1 μg/kg) or CRF plus AVP immediately after end of transportation and blood samples collected every 30 min for 3 h. The AP of animals transported for 0, 4 or 10 h were harvested for mRNA analyses. Plasma ACTH in transported animals increased within 1 h and remained elevated for 6 and 8 h versus NTC and 0 h values, respectively. Plasma concentrations of cortisol increased in response to transportation and remained elevated throughout the transport period. Injection of CRF or AVP to NTC animals increased plasma ACTH, but ACTH secretion in response to CRF or AVP was dramatically reduced in transported animals. ACTH secretion following co-injection of CRF and AVP tended to be less in transported animals, but was almost 100% greater than when secretagogues were administered separately. Despite decreased AP responsiveness to CRF and AVP, AP CRFR1 and V3 mRNAs were increased after 10 h transportation. Results indicate decreased AP responsiveness to CRF and AVP may regulate duration of ACTH secretion in response to transportation stress in cattle.